| Earthworks Art and Design Limited | Manufacturing | 1996 |
| East Green Child Care Services Limited | Administrative & support | 2002 |
| East Grinstead Upholstery Manufacturing Limited | | 1981 |
| East Sussex National Limited | Hospitality | 2000 |
| Eastbourne & Country Limited | Professional & scientific | 2001 |
| Eastbourne & Country Taxis Limited | Transport & logistics | 2000 |
| Eastbourne Car Auctions Limited | Wholesale & retail | 1967 |
| Easterbrook Surveying Ltd | Professional & scientific | 2007 |
| Easy Electronics Limited | Extraterritorial | 2012 |
| Easy TAN Limited | Real estate | 2005 |
| Easybay-Web Ltd | Administrative & support | 2008 |
| Echo Video Productions Limited | Information & communication | 2007 |
| Eclipse Cutters Limited | Manufacturing | 2006 |
| Eclipse Utilities Limited | Construction | 2006 |
| Eco Green Investments Limited | Construction | 2024 |
| Eco HI Solar Ltd | Construction | 2006 |
| Ecologika Limited | Information & communication | 1998 |
| Ecoluxe Ltd | Construction | 2007 |
| Ecom Media UK Limited | Manufacturing | 2002 |
| Ecstasy Foundation | Extraterritorial | 2006 |
| ED Burridge Engineering Limited | Professional & scientific | 2018 |
| Educational Psychologist Ltd | Education | 2005 |
| Edward Baden Limited | Transport & logistics | 1998 |
| Egerton Mercantile Limited | Wholesale & retail | 1997 |
| EGY Eagle Ltd | Administrative & support | 2012 |
| Eigen Limited | Information & communication | 2007 |
| Eight Sackville Road Management Limited | Households | 1994 |
| Eighteen Sussex Square Limited | Households | 1959 |
| Eighty Marina Limited | Professional & scientific | 1982 |
| EKO Consulting Ltd | Professional & scientific | 2003 |
| EL Mexicano Limited | Hospitality | 1998 |
| Electel Limited | Transport & logistics | 1988 |
| Electric Hair Limited | Administrative & support | 2008 |
| Electrical & Environmental Engineering Limited | Professional & scientific | 1993 |
| Elegant Dent Limited | Manufacturing | 2017 |
| Elegant Male Limited | Wholesale & retail | 2002 |
| Eleven Grafton Street Limited | Professional & scientific | 1999 |
| Elite Bodyshop Limited | Wholesale & retail | 2006 |
| Elite Software Company Limited | Information & communication | 1981 |
| Ellesridge Limited | Water & waste | 1990 |
| Ellman Henderson Limited | Real estate | 2005 |
| Elmo Trading Ltd | Wholesale & retail | 2003 |
| Elphick Bros. Plastering Limited | Construction | 1965 |
| Elphick Brothers Limited | Professional & scientific | 2005 |
| Elysium Accountancy Limited | Professional & scientific | 2003 |
| Elysium Leisure Limited | Extraterritorial | 2003 |
| Embar Marketing Limited | Financial services | 1989 |
| Emmkay Limited | Extraterritorial | 2006 |
| Employment International Limited | Information & communication | 2009 |
| Energy Flash Records Limited | Wholesale & retail | 2000 |
| Energy Technology Management Limited | Professional & scientific | 2006 |
| Enigma Titanium Ltd | Manufacturing | 2006 |
| Enquire International Ltd | Administrative & support | 2002 |
| Enterprise UN Limited | Hospitality | 2003 |
| Erchlis Ltd | Professional & scientific | 2022 |
| Eric Marchant & Co. - Patcham | | 1989 |
| Essential Detail Limited | Administrative & support | 2003 |
| Esuubi Trust | Education | 2006 |
| Ethos Engineered Design Limited | Manufacturing | 2007 |
| Eucaderm Limited | Manufacturing | 1992 |
| Euro Channel Logistics (UK) Limited | Transport & logistics | 2005 |
| Euroactive Trading Limited | Extraterritorial | 2003 |
| Eurocirtec Limited | Administrative & support | 1997 |
| European Cash Drawers Ltd | Wholesale & retail | 1996 |
| European Partners in Communication Ukeig | | 1990 |
| Europlants Limited | Wholesale & retail | 1998 |
| Eurotech Management Services Ltd | | 1989 |
| Everycare Eastbourne Limited | Administrative & support | 2003 |
| Evjc Limited | Health & social care | 2003 |
| Excalibur (Holdings) Limited | Professional & scientific | 1966 |
| Executive Accountancy Limited | Professional & scientific | 1990 |
| Exess Limited | Administrative & support | 1992 |
| Exess Property & Financial Services Ltd | Real estate | 1997 |
| Exit Designs Limited | | 1987 |
| Exmoor Country Homes Limited | Professional & scientific | 2004 |
| Extraspace Limited | Real estate | 1995 |
| F. Forte Developments Limited | Other services | 1975 |
| F.A. Welch (Hatton Garden) Limited | Other services | 1974 |
| Facade Options Limited | Wholesale & retail | 1991 |
| Fairfield Freehold Limited | Households | 2002 |
| Fairlene Management Limited | Construction | 1992 |
| Fairlight Garage Limited | Wholesale & retail | 2003 |
| Fairlight Heating Limited | Wholesale & retail | 1972 |
| Fairs & Green (Mechanical Services) Limited | Construction | 1995 |
| Fairs & Green Limited | Wholesale & retail | 1955 |
| Fairways Homes Limited | Construction | 2019 |
| Faiz Properties London Ltd | Real estate | 2022 |
| Falcon Airguns Limited | Professional & scientific | 2005 |
| Falinower Limited | Extraterritorial | 1973 |
| Family Assurance Staff Pension Scheme Trustees Ltd | Professional & scientific | 1971 |
| Farncombe Limited | Real estate | 1989 |
| Faronworth Limited | Households | 1978 |
| Fast Awake Limited | Extraterritorial | 2007 |
| Fast Heat International (U.K.) Limited | Manufacturing | 1987 |
| Fasten Your Seatbelt Productions Limited | Information & communication | 2005 |
| Favourite Pizza Ltd. | Hospitality | 2001 |
| Fawley Garage Limited | Wholesale & retail | 2003 |
| Fearless Computing Limited | Wholesale & retail | 2019 |
| Feetright Limited | Health & social care | 2003 |
| Feist Hedgethorne Limited | Professional & scientific | 2003 |