| 194 Ashley Down Road [Bristol] RTM Company Limited | Households | 2025 |
| 1MSG Ltd | Manufacturing | 2023 |
| 21DLE Ltd | Construction | 2014 |
| 24/34 CAM Pitch Management Company Limited | Households | 2022 |
| 26 Priory Road Gloucester Limited | Hospitality | 1995 |
| 49 at Italians Ltd | Hospitality | 2025 |
| 84/86 High Street CAM Management Company Ltd | Households | 2020 |
| A & a Stroud Taxis Limited | Transport & logistics | 1996 |
| A a Mason Limited | Manufacturing | 2008 |
| A Luong Ltd | Hospitality | 2023 |
| A Team Installations Limited | Construction | 2004 |
| A&R Scaffolding South West Limited | Construction | 2023 |
| A&S Productions Ltd | Administrative & support | 2025 |
| A.J.P. Electrical Limited | Construction | 1997 |
| ABS Electrical & Mechanical Limited | Manufacturing | 2025 |
| ABS Group Services Limited | Manufacturing | 2025 |
| ACA Taxis Ltd | Transport & logistics | 2023 |
| Acacia Ventures Ltd | Hospitality | 2021 |
| Accessence Ltd | Health & social care | 2024 |
| Action for Sustainability Ltd | Professional & scientific | 2012 |
| Active Connections Group Ltd | Arts & recreation | 2011 |
| Adapt Architects Limited | Professional & scientific | 2021 |
| Adventous Global Group Ltd | Professional & scientific | 2025 |
| Adventure Sports Media House Ltd | Information & communication | 2020 |
| Aeros Flying Club (Property) Limited | Professional & scientific | 1990 |
| Aesthetically Pleasing by JO Ltd | Other services | 2020 |
| Affordable Architecture Design Limited | Professional & scientific | 2015 |
| African Retail Services Ltd | Professional & scientific | 2022 |
| AFS (South West) Ltd | Manufacturing | 2008 |
| Aglow Building Systems Limited | Administrative & support | 1997 |
| Agnieszka ELK Ltd | Education | 2025 |
| Agri-Ecohealth Ltd. | Manufacturing | 2024 |
| Aipex Digital Ltd | Information & communication | 2023 |
| Air Land Integration Ltd | Professional & scientific | 2011 |
| AJP Commercial Ltd. | Professional & scientific | 2014 |
| AJW Solutions (UK) Limited | Manufacturing | 2008 |
| Aklye Ltd | Hospitality | 2025 |
| Alfa Aviation Limited | Professional & scientific | 1995 |
| Alison Joy Robinson Ltd | Health & social care | 2020 |
| Alpha ESS UK Limited | Manufacturing | 2020 |
| ALW Solutions Ltd | Construction | 2018 |
| Analisi Limited | Information & communication | 2011 |
| Andy Kinnear Consulting Ltd | Information & communication | 2020 |
| Angell Estates Ltd | Construction | 2020 |
| Anne Burrell Ltd | Professional & scientific | 2016 |
| Anti Damp Property Solutions Ltd | Construction | 2025 |
| Apex Patrol Medics Ltd | Health & social care | 2023 |
| Apollo Heating & Plumbing Limited | Construction | 2007 |
| Apsley House (Dursley) Property Management Ltd | Households | 2015 |
| ARB Solutions Ltd | Administrative & support | 2023 |
| ARC Ballistics Limited | Manufacturing | 2016 |
| ARC Manufacturing Solutions Limited | Manufacturing | 2006 |
| Archetypely Limited | Professional & scientific | 2019 |
| Areten Limited | Administrative & support | 2011 |
| Arlott Ltd | Hospitality | 2025 |
| Arnold Building Services Limited | Manufacturing | 1988 |
| Arriving in Style Ltd | Transport & logistics | 2024 |
| Asha AMA Ward Limited | Extraterritorial | 2022 |
| Ashen Plains LLP | | 2017 |
| Ashmead Solutions Limited | Information & communication | 2008 |
| ASK Plumbers Limited | Construction | 2015 |
| ASLAN1905 Ltd | Hospitality | 2025 |
| Asphalt Sports Surfacing Ltd | Construction | 2025 |
| Asphalt Surfacing Ltd | Construction | 2016 |
| AT Home South West Limited | Real estate | 2023 |
| Atkinson Construction Management Limited | Construction | 2022 |
| Augmentable Trading Ltd | Wholesale & retail | 2020 |
| Autoroute Shipping (Cheltenham) Ltd | Professional & scientific | 1989 |
| Auxus Ltd | Professional & scientific | 2018 |
| Avon Valley Gardens Limited | Administrative & support | 2022 |
| Avonports Laundry Services Ltd | Other services | 2014 |
| Axios Sports Ltd | Education | 2026 |
| Aysu Food Ltd | Hospitality | 2024 |
| Aztec Roadways Ltd | Construction | 2003 |
| B Squared Tuition Ltd | Education | 2014 |
| B.Walker & Co.(Dursley)Limited | Construction | 1960 |
| B2C Electrical Ltd | Construction | 2015 |
| B2C Estates Ltd | Real estate | 2025 |
| Baileys News Limited | Real estate | 1978 |
| Baileys News Properties Limited | Real estate | 2009 |
| Balcombe Productions Limited | Arts & recreation | 1958 |
| Bamboo Properties Limited | Real estate | 2006 |
| Bank Cafe Limited | Hospitality | 2017 |
| Bath Road Garage Ltd | Wholesale & retail | 2023 |
| BCF Saddlery Limited | Wholesale & retail | 2019 |
| BD BIM Services Ltd | Information & communication | 2025 |
| Beachguild Solutions Limited | Information & communication | 2007 |
| Bean’S Bags Ltd | Manufacturing | 2023 |
| Bearing Advisory Limited | Professional & scientific | 2026 |
| Beautiful KOI Limited | Agriculture & fishing | 2023 |
| Beermongery Inc Limited | Hospitality | 2025 |
| Beetle Back Designs Ltd | Arts & recreation | 2022 |
| Bencombe Tree Care Limited | Administrative & support | 2024 |
| Bennett Jones Partnership Limited | Real estate | 2015 |
| Berkeley Lime Ltd | Wholesale & retail | 2021 |
| Bernies Ventures Limited | Professional & scientific | 2024 |
| Big Space Heating Limited | Construction | 2026 |
| Bike Science Limited | Wholesale & retail | 2009 |
| Biscayne Consulting Limited | Professional & scientific | 2021 |
| Blade K Barber Limited | Other services | 2026 |