| Earthtec Environmental Consulting Ltd | Extraterritorial | 2010 |
| East Accommodation Limited | Real estate | 2017 |
| East Durham Electrical Ltd | Construction | 2025 |
| East Durham Guitars Ltd | Manufacturing | 2023 |
| East Durham Theatre Company Ltd | Administrative & support | 2024 |
| Eastbourne JV Limited | Construction | 2016 |
| Easter Spring Properties Ltd | Real estate | 2025 |
| Easy Flow TM Ltd | Construction | 2022 |
| Easy Home Solutions Ltd | Wholesale & retail | 2019 |
| EAT Fish Design Limited | Professional & scientific | 2015 |
| EATOO2026 Ltd | Hospitality | 2026 |
| Eazona Limited | Health & social care | 2026 |
| EB Wind Tech Ltd | Construction | 2023 |
| Ebac Management Services Limited | Extraterritorial | 1997 |
| Ebenezermorley Ltd | Hospitality | 2018 |
| Ebony Leisure Limited | Hospitality | 2008 |
| Ecco Electrical Contracting Company Ltd | Professional & scientific | 2022 |
| Echo Lettings NE Ltd | Real estate | 2023 |
| Echo Social Ltd | Professional & scientific | 2025 |
| Eco Country Parks Ltd | Hospitality | 2021 |
| Eco Life Ltd | Construction | 2020 |
| Eco Supermarket Limited | Wholesale & retail | 2019 |
| Eco Tech Renewables NE Ltd | Construction | 2025 |
| Eco-Vent TR19 Ltd | Administrative & support | 2022 |
| Ecoflow Plumbing Services Ltd | Construction | 2024 |
| Ecogreenia Limited | Professional & scientific | 2024 |
| Ecom-Fort Trade Ltd | Wholesale & retail | 2025 |
| Ecosound CIC | Arts & recreation | 2025 |
| Ecotyres Recycling Limited | Water & waste | 2019 |
| Ecowise Group Ltd | Information & communication | 2023 |
| Ed's Beds Limited | Wholesale & retail | 2015 |
| Edell Consulting Ltd | Professional & scientific | 2025 |
| Eden & Aeries Ltd | Real estate | 2023 |
| Eden Homes (Durham) Limited | Construction | 2015 |
| Edenplan Limited | Construction | 1994 |
| Edgemark Accounting Limited | Professional & scientific | 2025 |
| Ediecs Limited | Information & communication | 2006 |
| Edinburgh Coins Ltd | Wholesale & retail | 2025 |
| Edis E-Commerce Ltd | Wholesale & retail | 2025 |
| Educaitor Ltd | Information & communication | 2026 |
| Education Five Ltd | Education | 2017 |
| Education Matters (North East) Ltd | Education | 2015 |
| Education Plus NE Ltd | Education | 2016 |
| Ees(Uk) Ltd | Professional & scientific | 2013 |
| EFA Properties Ltd | Real estate | 2018 |
| Efendy Limited | Wholesale & retail | 2024 |
| EFM Consulting Ltd | Information & communication | 2012 |
| EGB Recycling Ltd | Water & waste | 2017 |
| Egnatia AL Ltd | Real estate | 2025 |
| EGW (North) Psychology Services Ltd | Health & social care | 2010 |
| Ehilcarr Distribution Ltd | Wholesale & retail | 2025 |
| EHS Response Ltd | Professional & scientific | 2007 |
| EHT Tax Accountants Limited | Professional & scientific | 2018 |
| Eight and Other Ltd | Wholesale & retail | 2025 |
| EJ Front Limited | Hospitality | 2022 |
| EJN Assets Limited | Administrative & support | 2011 |
| EJN Engineering Limited | Manufacturing | 2008 |
| Ekgl Global Ltd | Wholesale & retail | 2024 |
| Ekin Trade Ltd | Wholesale & retail | 2025 |
| EKM Group Ltd | Professional & scientific | 2024 |
| EL Eight Projects Limited | Construction | 2026 |
| EL Estribo Ltd | Real estate | 2023 |
| EL Foster Properties Ltd | Real estate | 2024 |
| EL Pincho Limited | Hospitality | 2019 |
| El-Roiadu Ltd | Hospitality | 2025 |
| Elan Eateries Limited | Hospitality | 2011 |
| ELB Asset Management Limited | Real estate | 2022 |
| ELC Cleaning Ltd | Administrative & support | 2022 |
| Elder Brook Park (Neasham) Residents Management Company Limited | Households | 2022 |
| Elevate Workplace Solutions Limited | Education | 2025 |
| ELG Developments Limited | Real estate | 2007 |
| Eliot & Neville Ltd | Construction | 2016 |
| Eliso Ltd | Health & social care | 2013 |
| Elite Escapes Northeast Ltd | Real estate | 2025 |
| Elite Fire Risk Assessments Ltd | Administrative & support | 2022 |
| Elite Landscaping NE Ltd | Administrative & support | 2020 |
| Elite Teams Limited | Administrative & support | 2017 |
| Ellerslie Group Limited | Financial services | 2022 |
| Ellie Wigham Entertainment Limited | Arts & recreation | 2023 |
| Elliott & Dean Consultancy Ltd | Professional & scientific | 2017 |
| Ellpro Ltd | Construction | 2025 |
| ELM Tree Durham Leisure Limited | Hospitality | 2019 |
| Elson Constructors Limited | Professional & scientific | 2008 |
| Elvet and Saddler (Properties) Ltd | Real estate | 2023 |
| Elvet and Saddler Restaurants Ltd | Hospitality | 2023 |
| Elvet Bars Limited | Hospitality | 2010 |
| Elvet Bridge Investments Limited | Hospitality | 2019 |
| Elvet Construction Consultants Ltd | Professional & scientific | 2007 |
| Elvet Investments Limited | Real estate | 2021 |
| Elvet Leisure Limited | Construction | 2025 |
| Elvet Recruitment Limited | Administrative & support | 2021 |
| Elvet Residences Limited | Real estate | 2016 |
| Elvetwood Ltd | Financial services | 2021 |
| Elvora by Somrat Ltd | Wholesale & retail | 2025 |
| Elysium Creative Arts CIC | Arts & recreation | 2022 |
| Embeded Consulting Ltd | Professional & scientific | 2025 |
| Emdg Healthcare Consultancy Ltd | Professional & scientific | 2026 |
| Emech Home Haven Ltd | Hospitality | 2025 |
| Emerson Black Consulting Limited | Administrative & support | 2021 |
| Emerson Fencing Ltd | Administrative & support | 2003 |