| 1295 Taxis Ltd. | Professional & scientific | 2018 |
| AC Access Solutions Ltd | Other services | 2016 |
| Agricultural Commodity Exchange Ltd | Wholesale & retail | 2013 |
| Alex Pheby Ltd | Administrative & support | 2025 |
| Allanton Arts Limited | Arts & recreation | 2025 |
| Allanton Inn Ltd | Hospitality | 2012 |
| Allsorts Childcare Centre Limited | Other services | 2005 |
| Amco Energy Limited | Construction | 1989 |
| Andrew Mcintosh Ltd | Construction | 2013 |
| ASA 23 Limited | Construction | 2023 |
| ASB Investments Ltd | Real estate | 2017 |
| Atanasov Care Homes Limited | Professional & scientific | 2023 |
| Awgtraining Ltd | Education | 2018 |
| B & K Hume Groundworks Limited | Construction | 2005 |
| Balneaves Limited | Wholesale & retail | 2018 |
| Bavs (Berwickshire) Ltd | Administrative & support | 2010 |
| Bawdengroup Ltd | Public administration | 2025 |
| Bccf Environmental | Other services | 2007 |
| Berwickshire Community Renewables LLP | | 2011 |
| BEV Marsh Farm Office Ltd | Professional & scientific | 2021 |
| Big Ryan's Engineering Ltd | Professional & scientific | 2023 |
| Bigson Property Ltd | Real estate | 2022 |
| Bitu-Tech Limited | Construction | 2014 |
| Blackadder 2025 Limited | Other services | 2025 |
| Blackadder Cottages Limited | Hospitality | 2025 |
| Blackadder Practice Limited | Professional & scientific | 2019 |
| Blackhill Windfarm Community Fund Limited | Other services | 2009 |
| Bluestone Farming Ltd | Agriculture & fishing | 2015 |
| Bonnyton Homes Ltd | Construction | 2022 |
| Border Embroideries Holdings Limited | Financial services | 2025 |
| Border Embroideries Limited | Wholesale & retail | 1990 |
| Border Reivers Racing Limited | Administrative & support | 2022 |
| Border Trade Services Ltd | Extraterritorial | 2020 |
| Borderland Tours Ltd | Administrative & support | 2023 |
| Borders Bike Training Ltd | Education | 2024 |
| Borders Windows & Doors Limited | Construction | 2025 |
| Bowshiel Ltd | Professional & scientific | 2024 |
| Brawbleu Ltd | Construction | 2021 |
| Browne White Ltd | Wholesale & retail | 2013 |
| Bruce Brown Builders Ltd | Construction | 2021 |
| Buildwell Construction Group Ltd | Construction | 2025 |
| C H Contracting Limited | Agriculture & fishing | 2000 |
| C H Haulage Limited | Information & communication | 2002 |
| Cacciamani Ltd | Professional & scientific | 2026 |
| Caldbeck Family Limited | Financial services | 2026 |
| Calmedical Ltd | Wholesale & retail | 2016 |
| Camenbeck Kennels Limited | Wholesale & retail | 2022 |
| Cannonball Investments Ltd | Real estate | 2018 |
| Capel Strategy Limited | Professional & scientific | 2015 |
| Celtic Driven Sporting Club Ltd | Arts & recreation | 2025 |
| Chris the Barber Ltd | Other services | 2003 |
| Clearsky Data Systems Ltd | Information & communication | 2025 |
| Cockburn Energy Ltd | Energy | 2013 |
| Coldingham Investments Limited | Arts & recreation | 2013 |
| Compass Experience Consulting Ltd | Professional & scientific | 2026 |
| Connect Berwickshire Youth Project | Other services | 2013 |
| Contract Cleaning Services (Borders) Ltd | Administrative & support | 2017 |
| Cradle AI Ltd | Information & communication | 2025 |
| Craigvinean Limited | Other services | 2025 |
| Cranshaws Camping Ltd | Hospitality | 2025 |
| Cranshaws Farm | | 1995 |
| Crimson Kitchens and Interiors Ltd | Construction | 2025 |
| Crucible Medtech Ltd | Professional & scientific | 2015 |
| David Finnie (Carpentry & Joinery) Ltd. | Construction | 2002 |
| David Renton & Sons (2000) Ltd. | Transport & logistics | 2000 |
| Deltak Limited | Administrative & support | 2016 |
| Denis Lawson Limited | Construction | 2002 |
| Diamond Financial (Scotland) Limited | Professional & scientific | 1999 |
| Diamond Financial Security Ltd | Financial services | 2011 |
| DM Timber Frame Ltd | Construction | 2024 |
| Doyle Accountancy Ltd | Professional & scientific | 2014 |
| Dream Cleaners Ltd | Administrative & support | 2024 |
| Duns Community Childcare CIC | Health & social care | 2022 |
| Duns Fish Limited | Hospitality | 2023 |
| Duns Lawnmower Centre Ltd | Manufacturing | 2020 |
| Dóchas Consulting Ltd | Professional & scientific | 2024 |
| E & AJ Robertson Limited | Extraterritorial | 2015 |
| E J N Developments Ltd | Construction | 2016 |
| E R Massie & SON Limited | Construction | 2006 |
| Edington Mill Limited | Households | 2009 |
| Edinshall Ltd. | Information & communication | 2020 |
| Edrom Associates Ltd | Professional & scientific | 2017 |
| Elba Charlotte Limited | Professional & scientific | 2013 |
| Elementary Property and Trading Ltd | Wholesale & retail | 2018 |
| Elementary Solutions Limited | Professional & scientific | 2014 |
| Ellemford Farms 2000 | | 2000 |
| Elmgen Ltd | Professional & scientific | 2026 |
| Ember & Thistle Limited | Wholesale & retail | 2011 |
| Engineering Outcomes Ltd | Professional & scientific | 2013 |
| Enhance Business Limited | Professional & scientific | 2003 |
| Established Events Ltd. | Hospitality | 2016 |
| Face PR C.I.C. | Professional & scientific | 2015 |
| Forbes Clinical Monitoring Limited | Professional & scientific | 2014 |
| Fort Glen Whisky Company Limited | Manufacturing | 2010 |
| Foston’S Butchery Limited | Manufacturing | 2019 |
| Frippy Jameson Sculpture Ltd | Arts & recreation | 2020 |
| Future Trees Ltd | Agriculture & fishing | 2019 |
| G.L.M.Romanes Limited | Wholesale & retail | 1978 |
| Genyops Ltd | Professional & scientific | 2024 |
| GET Software Holdings Limited | Information & communication | 2024 |