| Glenn Robertson Limited | Administrative & support | 2010 |
| Glensbury Limited | Wholesale & retail | 2011 |
| Greenwood Pigs Ltd | Agriculture & fishing | 2020 |
| Harcarse Hill Farm Ltd | Agriculture & fishing | 2024 |
| HCL Enterprises Ltd | Construction | 2022 |
| Hislop Commercial Vehicle Services Ltd. | Wholesale & retail | 2015 |
| Hislop Properties Ltd. | Real estate | 2015 |
| Hislop Storage Ltd. | Transport & logistics | 2015 |
| House of RSI Ltd | Information & communication | 2026 |
| Hunting Travel Club Limited | Agriculture & fishing | 2020 |
| Hurly-Burly Limited | Arts & recreation | 2009 |
| Ignorantia Juris Limited | Other services | 2018 |
| Interest Link Borders | Health & social care | 2006 |
| International Association for the Study of Child Language | Education | 2007 |
| Ivor Gaston & SON Ltd | Agriculture & fishing | 2012 |
| J & S Commercial Properties Limited | Real estate | 2022 |
| J B Cockburn Land Drainage & Groundworks Limited | Administrative & support | 2023 |
| J D Clark & Allan Limited | Professional & scientific | 2012 |
| J R & G W Thomson | | 1988 |
| J&L Electrical Ltd | Construction | 2024 |
| J. Aitchison (Duns) Limited | Wholesale & retail | 1961 |
| JBA Morrison Ltd | Real estate | 2020 |
| Jerry Energy Ltd | Transport & logistics | 2025 |
| Jetstone Ltd | Real estate | 2025 |
| Jodaal Limited | Professional & scientific | 2021 |
| John Thorburn & Sons Limited | Manufacturing | 2021 |
| Kenny Hall & SON Limited | Professional & scientific | 2024 |
| Kerrlett Construction Ltd | Construction | 2026 |
| Kevin Cowe Ltd | Agriculture & fishing | 2015 |
| Kincaid Builders Ltd | Construction | 2024 |
| KJC Aviation Ltd | Manufacturing | 2024 |
| Knowetap Construction Ltd | Construction | 2024 |
| L8PRO Ltd | Information & communication | 2026 |
| Land Custodians Ltd | Agriculture & fishing | 2020 |
| Leaf Scaffolding Ltd | Construction | 2020 |
| Liftbadminton Ltd | Arts & recreation | 2025 |
| Longformacus Community Park CIC | Other services | 2021 |
| LR Groundwork & Forestry Limited | Agriculture & fishing | 2020 |
| M & J Properties NE Ltd | Real estate | 2025 |
| M Bullard & SON Building Ltd | Construction | 2025 |
| Mackenzie Leather Edinburgh Ltd | Manufacturing | 2019 |
| Mansefield Property Trading Ltd | Extraterritorial | 2026 |
| Mansefield Residential Ltd | Extraterritorial | 2025 |
| Mark Haddon Ltd. | Information & communication | 2001 |
| Martin Bullard General Builders Ltd | Extraterritorial | 2021 |
| Meda Design Limited | Professional & scientific | 2015 |
| Melrose & Porteous Limited | Professional & scientific | 2018 |
| Merryhall Ltd | Professional & scientific | 2012 |
| Merse Consulting Ltd | Professional & scientific | 2016 |
| Mgck Music Limited | Information & communication | 2005 |
| Mill Farm Chirnside LLP | | 2019 |
| Mill Farm Stables Ltd | Arts & recreation | 2017 |
| Mill Garage (Duns) Limited | Wholesale & retail | 2006 |
| Millars Autos Ltd | Wholesale & retail | 2025 |
| Network Self Storage Ltd | Real estate | 2024 |
| Noly JOE Audio Ltd | Information & communication | 2025 |
| Old School Mastering Ltd | Information & communication | 2021 |
| Ortus Elan Ltd | Administrative & support | 2022 |
| P D Services Limited | Construction | 2016 |
| P8 Design Solutions Limited | Construction | 2025 |
| Parkour Outreach C.I.C. | Education | 2019 |
| Pearsons of Duns Limited | Wholesale & retail | 1998 |
| Pentland Lodge Limited | Agriculture & fishing | 2016 |
| Pittencrieff Limited | Construction | 2018 |
| Pittlesheugh Farm Bess Limited | Energy | 2022 |
| Premier Livestock Handling Ltd | Wholesale & retail | 2018 |
| Premier Specialist Cleaning Services Ltd | Administrative & support | 2019 |
| Primrosehill Sporting Limited | Arts & recreation | 2023 |
| Quinners Ltd | Financial services | 2026 |
| R & D Hope Investments Limited | Real estate | 2021 |
| R Chapman Services Ltd | Construction | 2021 |
| R. Logan Smith | | 1979 |
| R.M. Markby Limited | Construction | 2002 |
| Redpath Holdings Ltd | Extraterritorial | 2018 |
| Redpath Services Ltd | Extraterritorial | 2019 |
| Redpath Tool Hire Ltd | Administrative & support | 2014 |
| Redrock Lummels Ltd | Professional & scientific | 2020 |
| Reedyloch Construction Ltd | Construction | 2017 |
| Rehoboth Ventures Ltd | Health & social care | 2014 |
| Reiver Country Farm Foods Ltd. | Wholesale & retail | 1999 |
| Reiver Garage Services Limited | Wholesale & retail | 2014 |
| Renewable Technical Consultants Limited Limited | Professional & scientific | 2020 |
| Renton Tippers Limited | Construction | 2016 |
| Restigouche Ltd | Hospitality | 2019 |
| RF Construction (Duns) Limited | Construction | 2019 |
| RGB Holdings Ltd | Real estate | 2017 |
| Rmjfseed 0 Limited | Extraterritorial | 2023 |
| Robertson & Robertson Letting Ltd | Real estate | 2025 |
| Rotas Holdings Ltd | Financial services | 2022 |
| S D Hoof Trimming Ltd | Agriculture & fishing | 2020 |
| S. Lackenby General Builders Limited | Construction | 2002 |
| Scentiments Florist Ltd | Extraterritorial | 2023 |
| Scoger Ltd | Information & communication | 2023 |
| Scottish Clans and Castles Limited | Administrative & support | 2017 |
| Security in Sport International Ltd | Administrative & support | 2023 |
| SET Aside Greenlawdean Limited | Hospitality | 2025 |
| Silcock Property Ltd | Real estate | 2026 |
| Skeldons Transport Services Ltd | Transport & logistics | 2021 |
| Skyboat Investments Limited | Other services | 2025 |
| SL Engineering Solutions Ltd | Professional & scientific | 2024 |