| 1STOPFESTIVALSHOP Ltd | Wholesale & retail | 2025 |
| A Z Creative Limited | Wholesale & retail | 2019 |
| Absheron Limited | Real estate | 2022 |
| AJN Land Scotland PTE Ltd | Hospitality | 2019 |
| Alex Henry and Company GUN and Rifle Makers Limited | Professional & scientific | 1998 |
| Alex Martin GUN and Rifle Makers Limited | Manufacturing | 1999 |
| And.Scot Ltd | Administrative & support | 2023 |
| Anturus Communications Limited | Professional & scientific | 2016 |
| Aran Bakery Ltd | Manufacturing | 2017 |
| Atholl Arms Residence Limited | Hospitality | 2025 |
| Azura Dunkeld Limited | Hospitality | 2026 |
| Balogh Hotels Ltd | Manufacturing | 2018 |
| Basecamp Systems Ltd | Professional & scientific | 2025 |
| Bbfcn Limited | Hospitality | 2025 |
| Birch Mountain Furniture Ltd | Manufacturing | 2024 |
| Birken Tree (Scotland) Limited | Manufacturing | 2019 |
| Birnam CD Limited | Information & communication | 2011 |
| Birnam I Limited | Wholesale & retail | 2001 |
| Birnam Oak Cafe Ltd | Wholesale & retail | 2025 |
| Birnam Shop Ltd | Wholesale & retail | 2023 |
| Blood Eagle Security Services Limited | Administrative & support | 2017 |
| Bluwave Media Limited | Information & communication | 2021 |
| Brodies Timber Ltd | Manufacturing | 2010 |
| Bruges Renewables Limited | Extraterritorial | 2011 |
| BT Tax and Accountancy Ltd | Professional & scientific | 2025 |
| Burtec Limited | Wholesale & retail | 2021 |
| Caledonia Learning & Care Limited | Education | 2016 |
| Caledonian Rural Ltd | Professional & scientific | 2021 |
| Caledonian Wildlands Ltd | Other services | 2021 |
| Cally SAW Mill Ltd | Extraterritorial | 2011 |
| Cameron Hospitality Limited | Hospitality | 2025 |
| Capercaillie Ltd | Manufacturing | 2023 |
| Care Developments Livingston Ltd | Real estate | 2015 |
| Cathedral Coins and Collectables Ltd | Wholesale & retail | 2025 |
| Chapman Veterinary Service Limited | Wholesale & retail | 2019 |
| Colorfield Limited | Professional & scientific | 2024 |
| Corbenic Community Shop Ltd | Wholesale & retail | 2023 |
| Core Joinery and Interiors Limited | Construction | 2023 |
| Craggantoul Ltd | Hospitality | 2018 |
| Craig Daly Investments Limited | Other services | 2021 |
| Craiglush Homes Limited | Construction | 2020 |
| Creare Dunkeld Ltd | Real estate | 2025 |
| Creare Homes Limited | Real estate | 2021 |
| Dalfad Land Management Limited | Other services | 2022 |
| Dalguise Farms | | 1996 |
| Daniel Fraser & Co Limited | Professional & scientific | 1997 |
| Degw Limited | Extraterritorial | 2014 |
| DEW North Limited | Construction | 2007 |
| Drummond Fencing Limited | Manufacturing | 2006 |
| Dungarthill Heat Limited | Other services | 2017 |
| Dunkeld and Birnam Community Interest Company | Real estate | 2018 |
| Dunkeld and Birnam News CIC | Information & communication | 2024 |
| Dunkeld Builders Limited | Construction | 2009 |
| Dunkeld Distillery Limited | Manufacturing | 2021 |
| Dunkeld Fine Foods Ltd | Wholesale & retail | 2019 |
| Dunkeld Fish Bar Limited | Hospitality | 2025 |
| Dunkeld Medical Services Limited | Real estate | 2010 |
| Dunkeld Records Limited | Arts & recreation | 1986 |
| Dunkeld Smoke House Limited | Manufacturing | 2018 |
| Dunkeld Whisky Box Ltd | Wholesale & retail | 2018 |
| Eastwood Events Limited | Hospitality | 2012 |
| Ecoideam Limited | Professional & scientific | 2009 |
| Elizabeth Allison Interiors Limited | Wholesale & retail | 2006 |
| Elrick Studio Ltd | Professional & scientific | 2025 |
| EMC (Scotland) Ltd | Construction | 2017 |
| Equine Unlimited Scot Ltd | Agriculture & fishing | 2025 |
| Fair City Structural Consultants Limited | Professional & scientific | 2025 |
| FAR & Future Skies Ltd | Professional & scientific | 2026 |
| Fearbean Limited | Information & communication | 2025 |
| Fiona Valpy Ltd. | Other services | 2018 |
| FIX the Food Ltd | Hospitality | 2024 |
| Fletcher Financial Solutions Limited | Information & communication | 2022 |
| GA3 Associates Limited | Professional & scientific | 2023 |
| Gilroy HSE Ltd | Mining & quarrying | 2018 |
| Glenalmond Estates Ltd | Extraterritorial | 2019 |
| HAB Properties Ltd | Real estate | 2022 |
| Havard Advisory Ltd | Professional & scientific | 2023 |
| Highland Offroad (3) Limited | Arts & recreation | 2013 |
| Hill Robertson Communications LLP | | 2005 |
| Humble PIE Bakery Ltd | Extraterritorial | 2019 |
| Inchmagrannachan Enterprises Limited | Hospitality | 1976 |
| Inchmagrannachan Properties Limited | Real estate | 2016 |
| Invermill Farm LLP | | 2015 |
| James Macnaughton & SON Limited | Professional & scientific | 1995 |
| Jill Rogers Ltd | Education | 2024 |
| John Dickson & SON Limited | Wholesale & retail | 1936 |
| Kinnaird Estate LLP | | 2016 |
| KM Property Staging Ltd | Administrative & support | 2021 |
| Labora Property Company Limited | Construction | 2026 |
| Ladywell Fencing Ltd. | Construction | 2013 |
| Little Birnam Kitchen Limited | Manufacturing | 2020 |
| Little Trochry Limited | Hospitality | 2025 |
| Livvis Dunkeld Ltd | Hospitality | 2024 |
| Livvis Little Lunch Box Limited | Hospitality | 2020 |
| Ludos Moenus Ltd | Wholesale & retail | 2022 |
| Mary Arnold-Forster Ltd. | Professional & scientific | 2017 |
| Maws Holdings Limited | Financial services | 2018 |
| Mays of Dunkeld Ltd | Hospitality | 2024 |
| Mcintyre Warden Services Ltd | Administrative & support | 2021 |
| Morison Family Properties Ltd | Real estate | 2024 |