| Mortimer & SON GUN and Rifle Makers Limited | Professional & scientific | 1998 |
| MPD Dance School Ltd | Education | 2026 |
| Nausk Ltd | Real estate | 2019 |
| Neish Limited | Professional & scientific | 2014 |
| Newtyle Salmon Fishing Limited | Agriculture & fishing | 2023 |
| Outdoor Education Online Ltd | Education | 2019 |
| Packrafting Scotland Ltd | Arts & recreation | 2025 |
| Palmerstons (Dunkeld) Limited | Hospitality | 2022 |
| Palmerston’S Bespoke JAM Dunkeld Ltd | Wholesale & retail | 2024 |
| Perthshire Wellbeing Project Ltd | Health & social care | 2023 |
| Pica BLU Ltd | Wholesale & retail | 2023 |
| Pmmed Consultancy Limited | Health & social care | 2023 |
| Preston-Jones Ltd | Wholesale & retail | 2021 |
| Reyrey Woodworx Ltd | Manufacturing | 2021 |
| Rjloaf Ltd | Financial services | 2026 |
| Robin Fairholm Joinery Limited | Construction | 2017 |
| Rural Image Limited | Professional & scientific | 1999 |
| Rural PIE Co Ltd | Manufacturing | 2019 |
| Sanna Design Studio Ltd | Professional & scientific | 2025 |
| Savage Bytes Ltd | Information & communication | 2013 |
| Shannon’S WEE Shop Ltd | Wholesale & retail | 2024 |
| Strathbraan Limited | Agriculture & fishing | 2013 |
| Strathbraan Woodland Consulting Ltd | Agriculture & fishing | 2021 |
| Taurum Messenger Ltd | Information & communication | 2025 |
| TAY Bunkhouse Ltd | Hospitality | 2018 |
| TAY House (Scotland) Limited | Hospitality | 2016 |
| Taybank Growers Cooperative | Agriculture & fishing | 2017 |
| THE Canyoning Company Ltd | Arts & recreation | 2015 |
| THE Dunkeld Cleaning Company Ltd | Administrative & support | 2025 |
| THE Dunkeld Family Butcher Limited | Wholesale & retail | 2019 |
| THE Dunkeld VET Ltd | Professional & scientific | 2022 |
| THE Farmhouse B&B Ltd | Hospitality | 2019 |
| THE Naked Sheep Limited | Wholesale & retail | 2011 |
| THE WEE Van Company Ltd | Wholesale & retail | 2025 |
| Transition North Ronaldsay CIC | Agriculture & fishing | 2020 |
| Turing Festival Limited | Administrative & support | 2016 |
| Waterside Ecology LLP | | 2026 |
| Woodlandrose Limited | Manufacturing | 2026 |