| Virginia Assured Tenancies Limited | Households | 1999 |
| Virtual Customer Services (UK) Ltd | Administrative & support | 2018 |
| Virtue Direct Sales Ltd | Other services | 2015 |
| Visit Dunfermline Limited | Professional & scientific | 2008 |
| Visual Talks Ltd | Health & social care | 2021 |
| Vital Oxygen Limited | Health & social care | 2024 |
| Vitec Ltd | Manufacturing | 2001 |
| Vithill Ltd | Hospitality | 2026 |
| Vivas Tech Ltd | Information & communication | 2025 |
| Vizzini Bathrooms Ltd | Construction | 2010 |
| Vizzini Properties Limited | Real estate | 2023 |
| VN Holdings Limited | Professional & scientific | 2008 |
| Voiceboxed UK Ltd | Professional & scientific | 2024 |
| Vollood Hairdressing Limited | Other services | 2023 |
| VPI 18 Ltd | Households | 2026 |
| VR Holdings Fife Limited | Financial services | 2024 |
| Vtwin Production Services Ltd | Other services | 2015 |
| W & AS Bruce LLP | | 2023 |
| W Bruce Roofing Ltd | Construction | 2017 |
| W Henderson Roughcasting Ltd | Construction | 2018 |
| W R Inns Limited | Hospitality | 2011 |
| WAA Investments Ltd | Wholesale & retail | 2020 |
| Wallprint Scotland Ltd | Manufacturing | 2021 |
| Walls Electrical & Renewables Ltd | Manufacturing | 2016 |
| Walshy Joinery Limited | Construction | 2026 |
| Wanderlust Campers Ltd | Administrative & support | 2025 |
| Warbases Property Limited | Real estate | 2023 |
| Ward Consulting Services Limited | Financial services | 2015 |
| Watchman Alarms (Scotland) Limited | Administrative & support | 2009 |
| Waterproof Solutions Ltd | Construction | 2018 |
| Watson & Watson Ltd | Health & social care | 2022 |
| Watson Scott Properties Limited | Real estate | 2025 |
| Wayne Stores Limited | Wholesale & retail | 2022 |
| Wayne WEE Home Limited | Real estate | 2025 |
| Wbo&G Limited | Construction | 2024 |
| WD Innovation Consulting LLP | | 2025 |
| WD Innovation Group Ltd | Professional & scientific | 2021 |
| WE ARE Tingle Ltd | Hospitality | 2014 |
| WE Love Your Boiler Ltd | Construction | 2020 |
| Web Project Development Limited | Construction | 2015 |
| WEE Foragers Ltd | Hospitality | 2025 |
| WEE Tea Company Limited | Wholesale & retail | 2012 |
| Weestu Solutions Ltd | Manufacturing | 2025 |
| WEI Lucky Ltd | Hospitality | 2026 |
| Welcome Inn (Fife) Ltd | Hospitality | 2026 |
| Wellness Social Care Services Limited | Health & social care | 2021 |
| Weloveitaly Ltd | Hospitality | 2024 |
| West Cross Developments Ltd | Real estate | 2006 |
| West Fife Electrical Services Ltd | Construction | 2026 |
| West Fife Islamic Education and Welfare Centre Limited | Other services | 2018 |
| West Fife Store Ltd | Other services | 2016 |
| Westbrook Agencies Ltd | Wholesale & retail | 2024 |
| Westend Garage (Scotland) Limited | Extraterritorial | 2011 |
| Westgate Toon Ltd | Hospitality | 2026 |
| Whisky Walker Limited | Hospitality | 2022 |
| Whiskywid Ltd | Manufacturing | 2024 |
| White & Red FIX Ahead Ltd | Other services | 2026 |
| Whitehill Knowe Residents Limited | Households | 2026 |
| Whyte Contractors Ltd | Construction | 2017 |
| Wilco Developments Limited | Construction | 1987 |
| Wild Changer Ltd | Wholesale & retail | 2026 |
| Wild Rover Food Ltd | Hospitality | 2020 |
| Willcocks & Smart Limited | Other services | 2014 |
| Willcocks Roofing Limited | Construction | 2013 |
| William & Sons Logistics Ltd | Transport & logistics | 2026 |
| William Crowe Limited | Professional & scientific | 2015 |
| William Stephen (Bakers) Limited | Manufacturing | 1958 |
| Willow Pharmacy (Dunfermline) Ltd | Health & social care | 2010 |
| Wilson Bulk Haulage Ltd | Transport & logistics | 2021 |
| Wilson James Solutions Ltd | Construction | 2026 |
| Wilson Surfacing Contracts Limited | Construction | 2021 |
| WIN IT Retro Ltd | Wholesale & retail | 2025 |
| Wind Forge Ltd | Manufacturing | 2017 |
| Window Cleaning Co (Scotland) Ltd | Administrative & support | 2024 |
| Windowscene (Scotland) Ltd | Manufacturing | 2012 |
| WMR Developments Ltd | Real estate | 2026 |
| Wolf Manor Group Ltd | Professional & scientific | 2024 |
| Wolf Manor Studios Ltd | Professional & scientific | 2021 |
| Wolfpack Martial Arts 13 Ltd | Other services | 2020 |
| Wolfpack Martial Arts Ltd | Arts & recreation | 2019 |
| Wonderfoods Ltd | Hospitality | 2025 |
| Wonderoffood Limited | Hospitality | 2024 |
| Wood Property Services Ltd | Other services | 2016 |
| Woodhead Joinery Ltd | Construction | 2018 |
| Woodhead Property Ltd | Real estate | 2020 |
| Woodlea Bakery Ltd | Manufacturing | 2025 |
| Woodlea Farm Ltd | Agriculture & fishing | 2024 |
| Woodlea Farm Shop Ltd | Wholesale & retail | 2024 |
| Woodmill Healthcare Limited | Wholesale & retail | 2014 |
| Wool & Peckit Ltd | Agriculture & fishing | 2020 |
| Workbase Plus (UK) Ltd | Other services | 2016 |
| Worker Health Protection Ltd | Professional & scientific | 2019 |
| Working Woods Scotland Ltd | Other services | 2010 |
| WPR Investments Ltd | Financial services | 2025 |
| Wreaths in Bloom Limited | Wholesale & retail | 2024 |
| Wrightline (Road Marking) Ltd | Construction | 2015 |
| WS Fife Cleaners Ltd | Administrative & support | 2023 |
| Wymet Secretarial Services Limited | Extraterritorial | 2009 |
| Wynd Ltd | Hospitality | 2024 |
| X - OVO Limited | Professional & scientific | 2006 |