| TR Taxis Ltd. | Professional & scientific | 2000 |
| Track Plant Holdings Limited | Financial services | 2020 |
| Trade Heating Services Limited | Other services | 2016 |
| Trailblazing Gamechangers Limited | Administrative & support | 2023 |
| Train Like a Pro Limited | Education | 2025 |
| Trang Home Limited | Real estate | 2025 |
| Tranquillity Tax Limited | Professional & scientific | 2017 |
| Travel 24/7 Taxi Dunfermline Ltd | Transport & logistics | 2023 |
| Travelling Basket Company Ltd | Wholesale & retail | 2018 |
| Treasures Arbroath Limited | Wholesale & retail | 2022 |
| Treaton Limited | Real estate | 1999 |
| Treats From Abroad Limited | Wholesale & retail | 2023 |
| Trench Boxing & Fitness Ltd | Arts & recreation | 2019 |
| Trench Coaching Services Ltd | Arts & recreation | 2021 |
| Trenvo Digital Limited | Wholesale & retail | 2025 |
| Trident Access Ltd | Mining & quarrying | 2021 |
| Triple Tapped Ltd | Hospitality | 2023 |
| Trust Signals Ltd | Information & communication | 2023 |
| TSS PT Ltd | Hospitality | 2026 |
| TTJ Stores Ltd | Wholesale & retail | 2025 |
| TTM (2) Ltd | Real estate | 2025 |
| Tugarox Limited | Professional & scientific | 2014 |
| Tulchan Estates Ltd | Real estate | 1986 |
| Tullibole Developments Ltd. | Administrative & support | 2007 |
| Tullohill Accountancy Limited | Professional & scientific | 2020 |
| Tunch Foods Limited | Manufacturing | 2008 |
| Turhanwrtira Ltd | Wholesale & retail | 2024 |
| Turnbull Property Limited | Real estate | 2020 |
| Tusind TAK Limited | Wholesale & retail | 2020 |
| TVI Fife Limited | Wholesale & retail | 2025 |
| TWELVE30 Ltd | Real estate | 2021 |
| Two Spires Ltd | Hospitality | 2019 |
| Tyree Investments Limited | Construction | 2012 |
| TZA 1 Ltd | Transport & logistics | 2017 |
| UK Connective Ltd | Wholesale & retail | 2025 |
| UK Cora Trading Ltd | Transport & logistics | 2019 |
| Ulstein (U.K.) Limited | Manufacturing | 1974 |
| Ultimate Wellness Ltd | Administrative & support | 2013 |
| Ultrasoundechocardiography Ltd | Health & social care | 2026 |
| Underfoot Carpets & Floors Limited | Wholesale & retail | 2010 |
| Unique Ceremonial Services Ltd | Other services | 2017 |
| Unique Enterprise Ltd | Wholesale & retail | 2019 |
| Unique Flooring Fife Limited | Construction | 2011 |
| Uniroutes International Limited | Information & communication | 2020 |
| Unit Road Creative Ltd | Arts & recreation | 2024 |
| United in Fitness Ltd | Arts & recreation | 2022 |
| Universal Computing Ltd | Wholesale & retail | 2008 |
| Universal Utilities (Scotland) Limited | Professional & scientific | 2003 |
| UP North Foods Limited | Wholesale & retail | 2022 |
| UP to DO Ltd | Wholesale & retail | 2024 |
| Upstate Living Ltd | Wholesale & retail | 2026 |
| Upstream IT Limited | Information & communication | 2006 |
| Urban Golf Scot Ltd | Arts & recreation | 2025 |
| Urban Residential Letting Ltd | Real estate | 2016 |
| Urban Shop Ltd | Wholesale & retail | 2017 |
| Vacation Home Rental Ltd | Real estate | 2025 |
| Val-U-Blinds Ltd. | Manufacturing | 1986 |
| Valarcode Ltd | Information & communication | 2026 |
| Valen Grading Ltd | Wholesale & retail | 2025 |
| Valinex Limited | Administrative & support | 2018 |
| Valinor Wealth Limited | Financial services | 2025 |
| Valler UKB Properties Ltd | Households | 2025 |
| Valley Electrical Ltd | Construction | 2021 |
| Valley Grocers Ltd | Wholesale & retail | 2025 |
| Valleyfield Community Club | Arts & recreation | 2014 |
| Vanguard Maintenance Ltd | Construction | 2022 |
| Vaughan Electrical Services (Scotland) Limited | Extraterritorial | 1985 |
| Vault Capital Limited | Other services | 2020 |
| Vault Property Ltd | Real estate | 2017 |
| Vauxhallpartsuk Limited | Water & waste | 2012 |
| Vaxcavation Ltd | Construction | 2026 |
| VC Fitness Limited | Arts & recreation | 2015 |
| VCR Fabrication Ltd | Construction | 2019 |
| VEA Medical Ltd | Health & social care | 2019 |
| Velocity 44 Limited | Wholesale & retail | 2010 |
| Venair Ltd | Administrative & support | 2010 |
| Veneno Media Ltd | Wholesale & retail | 2025 |
| Venue Viability Ltd | Hospitality | 2024 |
| Versilia Limited | Real estate | 2015 |
| Vert Rotors UK Limited | Manufacturing | 2013 |
| Verve Bars Ltd | Hospitality | 2024 |
| Verysfy Ltd | Wholesale & retail | 2023 |
| VET Extra Ltd | Agriculture & fishing | 2006 |
| Veto GK Ltd | Wholesale & retail | 2025 |
| Vetrica Ltd | Professional & scientific | 2011 |
| Vets NOW Emergency Limited | Professional & scientific | 2001 |
| Vets NOW Limited | Professional & scientific | 2008 |
| Vettraino Healthcare Limited | Health & social care | 2022 |
| Viaden Energy Consultants Limited | Professional & scientific | 2018 |
| Vibe Hair Styling Limited | Other services | 2014 |
| View.One Ltd | Information & communication | 2025 |
| Viken Ltd. | Professional & scientific | 2002 |
| Vilana NB Ltd | Wholesale & retail | 2017 |
| Village Taverns Limited | Hospitality | 2024 |
| Villamill Digital Limited | Information & communication | 2016 |
| Villamill Limited | Information & communication | 2015 |
| Vintage and Vogue Ltd | Wholesale & retail | 2026 |
| Violet Aesthetics Limited | Health & social care | 2025 |
| Violet Autos Ltd | Wholesale & retail | 2021 |
| VIP Property Investment Limited | Financial services | 2023 |