| UP Exterior Cleaning Ltd | Administrative & support | 2022 |
| UP Pro Ltd | Administrative & support | 2019 |
| Upmill Enterprises L.P. | | 2015 |
| Upper Dens Landscaping Limited | Households | 2000 |
| Upperkirkgate Limited | Hospitality | 2013 |
| Uppertunity Limited | Health & social care | 2015 |
| Upstream Research Ltd | Other services | 2024 |
| Uptay Ltd | Information & communication | 2024 |
| Uptrans Consulting L.P. | | 2016 |
| UR Alza Ltd | Transport & logistics | 2025 |
| Urban Crime Ltd | Administrative & support | 2026 |
| Urban Investments Dundee Ltd | Real estate | 2020 |
| Urban Peak Roofing Ltd | Construction | 2026 |
| Urban Print (Dundee) Ltd | Manufacturing | 2013 |
| Urban Pro Stay Ltd | Real estate | 2025 |
| Urban Quick Cart Ltd | Wholesale & retail | 2025 |
| URBAN-2P- Limited | Hospitality | 2023 |
| Urbana Africa Ltd | Wholesale & retail | 2026 |
| Urekarma Property Group Ltd | Real estate | 2021 |
| Urugo Ltd | Wholesale & retail | 2026 |
| Uscf Ltd | Construction | 2023 |
| USH Properties Ltd | Real estate | 2019 |
| Utili-Train Group Limited | Professional & scientific | 2025 |
| Utility Connect Northern Ltd | Construction | 2023 |
| V & I Joinery Ltd | Construction | 2022 |
| V & S Properties Tayside Limited | Real estate | 1996 |
| V D N Engineering Limited | Professional & scientific | 2011 |
| V&D Garage Ltd | Wholesale & retail | 2023 |
| V&F Dundee Ltd | Transport & logistics | 2019 |
| V&V Property Development (2) Ltd | Construction | 2026 |
| V&V Property Development Ltd | Real estate | 2023 |
| V&Z Food Limited | Hospitality | 2022 |
| Vadar Autotech Ltd | Wholesale & retail | 2019 |
| Vahin Enterprise Limited | Health & social care | 2018 |
| Vale Newsagents & Grocers Ltd | Wholesale & retail | 2018 |
| Valente Properties LLP | | 2017 |
| Valente-Wallace Properties Limited | Real estate | 2025 |
| Valentine Management Services Ltd | Professional & scientific | 2025 |
| Valentine Motors Dundee Ltd | Wholesale & retail | 2017 |
| Valentines of Dundee Limited | Extraterritorial | 1907 |
| Valtell International LP | | 2017 |
| Values Impact CIC | Professional & scientific | 2026 |
| Vandan Ltd | Construction | 2025 |
| Vantage Linguists Limited | Professional & scientific | 2026 |
| Vantel Limited | Administrative & support | 2026 |
| Vantyx Services Ltd | Real estate | 2026 |
| Varion Scientific Limited | Professional & scientific | 2026 |
| Vascular Flow Technologies Limited | Manufacturing | 1998 |
| Vaveva Ltd | Real estate | 2018 |
| Vayanis Design Ltd | Information & communication | 2019 |
| VBN Traders Ltd | Wholesale & retail | 2024 |
| VC Motors Dundee Ltd | Wholesale & retail | 2025 |
| VC Render Ltd | Construction | 2026 |
| VCH Tayside Ltd | Hospitality | 2016 |
| Vcodex Holdings (NO.2) Limited | Financial services | 2017 |
| Vcodex Limited | Information & communication | 2007 |
| VD Rentals Ltd | Other services | 2020 |
| VDK Electrical Services Limited | Professional & scientific | 2014 |
| VDR Auto Limited | Wholesale & retail | 2026 |
| VDR Renovation Ltd | Construction | 2023 |
| VDS Consultancy Limited | Mining & quarrying | 2018 |
| Vector Leisure Ltd | Hospitality | 2021 |
| Vehicle Assistance Network Limited | Wholesale & retail | 2010 |
| Velinkx Ltd | Manufacturing | 2024 |
| Veluno Ltd | Wholesale & retail | 2025 |
| Venezia Entertainments Limited | Other services | 2024 |
| Ventilation & Maintenance Solutions Ltd | Manufacturing | 2014 |
| Venture AH36 Limited | Agriculture & fishing | 2023 |
| Venture Strategic Development Ltd | Extraterritorial | 2015 |
| Ventus Trading LP | | 2015 |
| Venue LAB Hospitality Ltd | Professional & scientific | 2025 |
| Veracore Renewables Ltd | Manufacturing | 2025 |
| Verdala Property Limited | Real estate | 2020 |
| Verdant Analytix Ltd | Information & communication | 2024 |
| Veritas Mortgage Finance Solutions Ltd | Financial services | 2022 |
| Verona Foods Edinburgh Ltd | Hospitality | 2019 |
| Versa Business Support Ltd | Administrative & support | 2024 |
| Versa Capital LP | | 2015 |
| Versa Tiling & Flooring Ltd | Wholesale & retail | 2026 |
| Versapro Limited | Transport & logistics | 2024 |
| Vertaenergy Ltd | Manufacturing | 2024 |
| Vertex Carnoustie Limited | Hospitality | 2019 |
| Vertical Current Ltd | Construction | 2026 |
| Vertical Growing Ltd | Agriculture & fishing | 2025 |
| Vertreka Travel Ltd | Administrative & support | 2025 |
| Verum Forensic Consultancy Limited | Administrative & support | 2019 |
| Verus Financial Planning Limited | Financial services | 2005 |
| Verus Trustee Company Limited | Professional & scientific | 2023 |
| Very Evil Demons Ltd | Information & communication | 2024 |
| Vetswap Ltd | Administrative & support | 2014 |
| Vexberg Import L.P. | | 2015 |
| Vexform Inter L.P. | | 2015 |
| Veyda Ltd | Real estate | 2017 |
| VF Services ( Scotland ) Ltd | Construction | 2025 |
| VF Solar Renewables Ltd | Construction | 2025 |
| Vfbo Ltd | Wholesale & retail | 2022 |
| Vibe Tech Repairs Ltd | Other services | 2026 |
| Vicki LOW Physiotherapy Ltd | Health & social care | 2022 |
| Vicky Food Ltd | Manufacturing | 2019 |
| Victoria Bar Ltd | Hospitality | 2023 |