| Tshirtprinting Ltd | Manufacturing | 2009 |
| TSM CAB Ltd | Transport & logistics | 2016 |
| Tspc Holdings Limited | Professional & scientific | 2005 |
| Tstrachan Logistics Ltd | Transport & logistics | 2025 |
| TTD Limited | Professional & scientific | 2009 |
| Tteb Limited | Real estate | 2004 |
| TTS (Scot) Ltd | Hospitality | 2022 |
| Tubular Inspection Products Ltd. | Manufacturing | 2007 |
| Tudbic OIL & GAS Ltd | Professional & scientific | 2018 |
| Tuilz Property Limited | Real estate | 2022 |
| Tuko Baby Ltd | Wholesale & retail | 2023 |
| Tula Property Investment Ltd | Financial services | 2018 |
| Tula Property Ltd | Real estate | 2016 |
| Tula Studio Ltd | Administrative & support | 2025 |
| Tulchan of Glenisla Forest Limited | Agriculture & fishing | 1994 |
| Tullibardine Smiddy Ltd | Manufacturing | 2021 |
| Tullideph Dundee Limited | Hospitality | 2022 |
| Tullitec Limited | Information & communication | 2008 |
| Tulloch Enterprises Ltd | Administrative & support | 2019 |
| Tullyherron Properties Limited | Administrative & support | 2014 |
| Tulyar Properties Limited | Real estate | 2005 |
| Tupacion LP | | 2014 |
| Turblown Limited | Information & communication | 1993 |
| Turbochange Ltd | Professional & scientific | 2018 |
| Turbokast Ltd | Information & communication | 2026 |
| Turner & Hooch Limited | Hospitality | 2017 |
| Turner (Dundee) Limited | Construction | 1985 |
| Turriff Taxis Ltd | Administrative & support | 2015 |
| Turuq Ltd | Professional & scientific | 2017 |
| Tweed Financial Planning Limited | Financial services | 2020 |
| Twelve Fifty One Ltd | Information & communication | 2016 |
| Twenty 6 Seven Training Limited | Education | 2026 |
| Twinex Energy L.P. | | 2015 |
| Twinfield Invest L.P. | | 2015 |
| Twinkle Tales Ltd | Other services | 2024 |
| Two J'S Taxis Limited | Transport & logistics | 2010 |
| Two M & H Limited | Financial services | 2011 |
| TX Healthcare Ltd | Health & social care | 2025 |
| TXL Import L.P. | | 2015 |
| Tycoon Capital LP | | 2016 |
| Tyndrum Holiday Park Limited | Hospitality | 2017 |
| Typhon One Properties Limited | Real estate | 2020 |
| Typhon Properties Limited | Real estate | 2019 |
| Tyre Fighter Commercial Ltd | Wholesale & retail | 2023 |
| Tyremax Pro Ltd | Wholesale & retail | 2025 |
| U & H Investments Limited | Real estate | 2010 |
| U & Q Properties Ltd | Real estate | 2026 |
| U Mateusza Ltd | Wholesale & retail | 2020 |
| U-Neek Services Ltd | Water & waste | 2017 |
| Uberscaff Ltd | Construction | 2017 |
| UBG Sales L.P. | | 2014 |
| UBO Direct Ltd | Transport & logistics | 2020 |
| Ubrex Investment LP | | 2017 |
| UF03 Ltd | Hospitality | 2024 |
| UH Retail Ltd | Wholesale & retail | 2023 |
| UH Traders Ltd | Wholesale & retail | 2019 |
| UIK Dundee Ltd | Hospitality | 2024 |
| UK Design Studio Ltd | Manufacturing | 2024 |
| UK Green Edge Gardens Ltd | Administrative & support | 2025 |
| UK Line and Surface Ltd | Construction | 2026 |
| UK Tour Protection Ltd | Administrative & support | 2023 |
| Ukamart Global Ltd | Wholesale & retail | 2025 |
| UKE Universe Ltd | Wholesale & retail | 2026 |
| Ultradiagnostics Fife Ltd | Public administration | 2026 |
| Ultrakleen Car Valetting Limited | Administrative & support | 2018 |
| Ultramech Ltd | Construction | 2022 |
| Ulyatt Medical Limited | Health & social care | 2019 |
| Umbra Dynamics Ltd | Information & communication | 2024 |
| Umpteen Strings Limited | Public administration | 2015 |
| Umza Ltd | Wholesale & retail | 2026 |
| Undercranked Limited | Administrative & support | 2012 |
| Unibrand Commercial L.P. | | 2015 |
| Unicar Alliance L.P. | | 2011 |
| Unicom Communications Limited | Administrative & support | 2012 |
| Unicom Design Solutions Ltd | Information & communication | 2025 |
| Unicroft Trade L.P. | | 2016 |
| Unidoorsuk Ltd | Wholesale & retail | 2021 |
| Uniglobe Sales L.P. | | 2011 |
| Unigroup Media Ltd | Information & communication | 2017 |
| Union Street Bar Limited | Hospitality | 2023 |
| Union Street Developments Ltd. | Administrative & support | 1999 |
| Union Street Grocers Ltd | Wholesale & retail | 2025 |
| Union Street Investments Ltd. | Administrative & support | 2009 |
| Union Street Properties Ltd. | Construction | 1998 |
| Unistar Commerce L.P. | | 2015 |
| Unit 21 Ltd | Information & communication | 2023 |
| Unit Strong Fitness Ltd | Arts & recreation | 2022 |
| United Brethren Mission | Education | 2005 |
| Univ Learn Limited | Professional & scientific | 2025 |
| Universal Engineering Tools Limited | Wholesale & retail | 2014 |
| Universal Foods Dundee Ltd | Hospitality | 2022 |
| Universal Supermarket Ltd | Wholesale & retail | 2022 |
| Universal Supplies North Limited | Manufacturing | 2020 |
| Universe Corporation L.P. | | 2015 |
| University of Dundee Nursery Limited | Administrative & support | 2002 |
| University of Dundee Superannuation & Life Assurance Scheme Trustees Limited | Financial services | 2021 |
| Unknown Pleasures (Edinburgh) Ltd | Wholesale & retail | 2012 |
| Unmanned Organisation Ltd | Professional & scientific | 2025 |
| UNO Installations Limited | Construction | 2023 |
| UOD Enterprises Limited | Education | 2022 |