| Stirling Dog Day Centre - Retail Limited | Other services | 2021 |
| Stonehouse Trading Limited | Wholesale & retail | 2019 |
| Strategic Innovative Finance Ltd. | Financial services | 2004 |
| Strathearn Grain Company Ltd. | Administrative & support | 2009 |
| Strathtay Limited | Financial services | 1988 |
| Stratified Medical Communications Ltd | Professional & scientific | 2013 |
| Streetwise Properties Ltd | Real estate | 2018 |
| Strikepoint Studios Ltd | Information & communication | 2026 |
| Stuart, Mcintyre & Stuart (Oakburn Garage) Limited | Wholesale & retail | 1967 |
| Studio M Ltd | Arts & recreation | 2019 |
| Subsea Services Solutions Ltd | Professional & scientific | 2018 |
| Sukhi Kaur Consulting Ltd | Professional & scientific | 2024 |
| Sunny Connections CIC | Education | 2023 |
| Sunny Horizons Ltd | Education | 2022 |
| Suspend Fitness Ltd | Arts & recreation | 2016 |
| Sustainability Catalyst Ltd | Professional & scientific | 2012 |
| Sutherland Launch Ltd | Transport & logistics | 2024 |
| Swimvibe Ltd | Administrative & support | 2014 |
| Sylvan Woodlands Limited | Hospitality | 2020 |
| T and K Builders Limited | Construction | 2001 |
| Tality Edinburgh Limited | Other services | 2026 |
| Technical Paraplanning Solutions Ltd | Public administration | 2024 |
| Teentalkscotland Ltd | Education | 2018 |
| THE Cleaning Clan Ltd | Administrative & support | 2024 |
| THE Dunblane Sports Club Limited | Arts & recreation | 1979 |
| THE Gold Post Box CIC | Wholesale & retail | 2021 |
| THE Golden Bottom Bakery Ltd | Manufacturing | 2022 |
| THE Golf Inn Auchterarder Ltd. | Hospitality | 2013 |
| THE Groom Shack Throsk Ltd | Wholesale & retail | 2023 |
| THE Mustard Seed (Restaurants) Limited | Hospitality | 2000 |
| THE Play Therapy Haven Ltd | Professional & scientific | 2024 |
| THE Power of Three (Scotland) Ltd | Wholesale & retail | 2016 |
| THE Practice Sauna and Ice Ltd | Other services | 2026 |
| THE Process Builder Ltd | Professional & scientific | 2014 |
| THE TIN CAN Cafe Ltd | Hospitality | 2023 |
| Themacdogfactory Ltd | Wholesale & retail | 2012 |
| Thirty Degrees Inverness Limited | Other services | 2017 |
| Thomas Elder & Sons (Stirling) Limited | Real estate | 1933 |
| Tickety BOO Coaching Limited | Professional & scientific | 2016 |
| Tickety BOO People Limited | Professional & scientific | 2017 |
| Tickety BOO Training Limited | Hospitality | 2009 |
| Tigh NA Mara Salen Ltd | Hospitality | 2013 |
| TIM Newman Ltd | Professional & scientific | 2024 |
| TIMBUK3 Projects Ltd. | Mining & quarrying | 2011 |
| Time 2 Help Ltd. | Health & social care | 2012 |
| Tirrick Animation Limited | Information & communication | 2019 |
| Top Tails Dogs Ltd | Extraterritorial | 2024 |
| Top Tails Neurodevelopment Clinic CIC | Health & social care | 2026 |
| Tournesol Services Limited | Real estate | 2021 |
| Town & Country Roofing Services Limited | Construction | 1996 |
| Tracks (Scotland) Ltd | Administrative & support | 2021 |
| Trailwise Travel Limited | Administrative & support | 2020 |
| Triple Thread Productions Ltd | Arts & recreation | 2025 |
| Tscambler Ltd | Health & social care | 2024 |
| TT Business Solutions Ltd | Professional & scientific | 2013 |
| Tunnelmed Services Ltd | Health & social care | 2018 |
| Turadh Hospitality Ltd | Real estate | 2025 |
| Turadh SCO Ltd | Real estate | 2026 |
| Turadh SCO2 Ltd | Hospitality | 2026 |
| Tursa Property Ltd | Professional & scientific | 2012 |
| Tweskard Ltd | Wholesale & retail | 2023 |
| Unwind Away Ltd | Administrative & support | 2024 |
| Upper Craigs NO1 Ltd | Hospitality | 2019 |
| VA Foodservice Ltd. | Wholesale & retail | 2022 |
| VA Origins Ltd | Financial services | 2022 |
| Valour Games Ltd | Wholesale & retail | 2026 |
| Victoria Stewart Yoga Ltd | Arts & recreation | 2017 |
| Village Inn Dunblane Ltd | Other services | 2017 |
| Voicebusiness Training Ltd | Administrative & support | 2022 |
| Voso Limited | Information & communication | 2025 |
| Wags Pet Grocer Group Ltd | Manufacturing | 2025 |
| Wang Good Food Ltd | Hospitality | 2026 |
| Wattgen Ltd | Other services | 2017 |
| Weigh CIC | Wholesale & retail | 2019 |
| Wellbeing 4 ALL (Scotland) Ltd | Other services | 2026 |
| Westburn Consulting Ltd | Professional & scientific | 2019 |
| Westy Bofa Limited | Hospitality | 2019 |
| White Boar Interiors Ltd | Manufacturing | 2020 |
| Wider Aspect Innovation Limited | Professional & scientific | 2008 |
| Wild Tracks Global Ltd | Administrative & support | 2025 |
| Wildigital Ltd | Information & communication | 2025 |
| William Boles Ltd | Wholesale & retail | 2024 |
| Wine OTT Limited | Wholesale & retail | 2024 |
| Wine Shops International Limited | Wholesale & retail | 2025 |
| With the Waves Limited | Professional & scientific | 2015 |
| Witr Networks Ltd | Information & communication | 2024 |
| Wizr Ltd | Information & communication | 2021 |
| Yeezy Limited | Wholesale & retail | 2019 |
| Yoga With Yvonne Ltd | Health & social care | 2024 |
| YOU ARE Never Limited | Information & communication | 2026 |
| YS1 Rentals Limited | Real estate | 2022 |