| Pragmatical Ltd. | Professional & scientific | 2024 |
| Prehoc Ltd | Information & communication | 2021 |
| Premier Windscreens Scotland Ltd. | Extraterritorial | 2022 |
| PREP2PERFORM Ltd | Education | 2020 |
| PREP2PERFORM Physiotherapy & Sports Performance Ltd | Health & social care | 2021 |
| Prestige Car Carriers Ltd | Transport & logistics | 2024 |
| Prolisto Ltd | Information & communication | 2026 |
| Prometheus Pharma Consulting Limited | Professional & scientific | 2015 |
| Property Investment Scotland Ltd | Construction | 2025 |
| Puro Environmental Limited | Professional & scientific | 2019 |
| Purple Bear Hospitality Company Ltd. | Other services | 2017 |
| Queen BEE Beauty Limited | Other services | 2026 |
| R Wilkins Development Services Limited | Administrative & support | 2012 |
| R&S Shepherd Ltd | Professional & scientific | 2021 |
| R.B. Distilled Strategy Ltd | Professional & scientific | 2025 |
| Railfreight Scotland Ltd | Extraterritorial | 2020 |
| RAM Interiors Scotland Limited | Construction | 2013 |
| Reay Dunlop Limited | Professional & scientific | 2007 |
| Reconstructor Limited | Construction | 2007 |
| Red & Co Services Ltd | Other services | 2022 |
| Red Sphere Property Limited | Real estate | 2016 |
| Redgate Associates Ltd | Professional & scientific | 2017 |
| Redline Planning Services Ltd | Professional & scientific | 2021 |
| Remarind Limited | Professional & scientific | 2026 |
| Rennie Media Ltd. | Other services | 2018 |
| Resilience Restore Group Ltd | Information & communication | 2025 |
| Resilient Economy CIC | Professional & scientific | 2026 |
| Restless Heart Ltd | Wholesale & retail | 2024 |
| Ristol Consulting Ltd | Professional & scientific | 2013 |
| Rlsat Limited | Real estate | 2025 |
| Robertson Jones Wealth Management Limited | Financial services | 2007 |
| Robertsons Tax Ltd | Professional & scientific | 2010 |
| Room to Glow Ltd | Other services | 2024 |
| Roots Cycles Ltd | Wholesale & retail | 2018 |
| Rosedale Hospitality Ltd | Hospitality | 2023 |
| Ross Anderson (Stirling) Limited | Information & communication | 2014 |
| Ross Lettings Limited | Real estate | 2020 |
| RRS Property Ltd | Real estate | 2023 |
| RSA Leisure Ltd | Hospitality | 2023 |
| Ruby Ruby Loves Ltd | Wholesale & retail | 2024 |
| Rukwa Limited | Financial services | 2017 |
| Rumblane Ltd | Extraterritorial | 2021 |
| Ryvoan Property Ltd | Real estate | 2021 |
| S2 Partners Limited | Professional & scientific | 2005 |
| Safe Haven Therapy CIC | Health & social care | 2025 |
| Sahih Limited | Health & social care | 2017 |
| Sally Shaw User Research Limited | Professional & scientific | 2021 |
| Santa Cruz Kustom Limited | Other services | 2025 |
| Saor Limited | Wholesale & retail | 2024 |
| SAS Vascular Ltd | Health & social care | 2022 |
| Saurus Healthcare Ltd. | Professional & scientific | 2016 |
| Scenario Associates Limited | Professional & scientific | 2011 |
| Schema Therapy Scotland Ltd | Health & social care | 2025 |
| Sckc Ltd | Other services | 2019 |
| Scogo Ltd | Professional & scientific | 2025 |
| Scotia Apps Ltd | Information & communication | 2015 |
| Scotia Property Management Ltd | Real estate | 2024 |
| Scotonomics Ltd | Information & communication | 2022 |
| Scottitconsulting Limited | Information & communication | 2024 |
| Sdbc Ltd | Education | 2023 |
| Seaglass Cottage Limited | Hospitality | 2025 |
| Second-Hand Ltd | Wholesale & retail | 2007 |
| Seluna Ltd | Professional & scientific | 2022 |
| Seriously Easy Ltd | Wholesale & retail | 2014 |
| Seven Apart Limited | Hospitality | 2018 |
| Sgian Ltd | Administrative & support | 2025 |
| Shaun's Garage Limited | Wholesale & retail | 2026 |
| Shawara Ltd | Professional & scientific | 2018 |
| Silva & Silva Shetland Ltd | Professional & scientific | 2025 |
| Simbell Land & Property Management Limited | Real estate | 2021 |
| Simply Culture Limited | Professional & scientific | 2022 |
| Sjrennie Consulting Ltd | Professional & scientific | 2012 |
| SK Contract Solutions Limited | Professional & scientific | 2014 |
| Skaldiq Technologies Limited | Information & communication | 2026 |
| Skein Management Ltd | Professional & scientific | 2020 |
| SKS Scotland Community Interest Company | Professional & scientific | 2009 |
| Smile Antalya UK Ltd | Professional & scientific | 2022 |
| Sole City GB Ltd | Wholesale & retail | 2022 |
| Sonas Reserves Ltd | Wholesale & retail | 2021 |
| Sonas Stays Ltd | Real estate | 2022 |
| Soutra Ltd | Professional & scientific | 2010 |
| Spaceport Alba Nova Ltd | Transport & logistics | 2025 |
| Spalding Renovations Ltd | Real estate | 2025 |
| Splashscape Ltd. | Administrative & support | 2026 |
| Sports Design 123 Ltd | Other services | 2023 |
| Spyn Ltd | Professional & scientific | 2024 |
| SRC Landscapes Limited | Administrative & support | 2010 |
| Stanger Pro Limited | Professional & scientific | 2015 |
| Stealth Finance Ltd | Financial services | 2020 |
| Steelfab Services Ltd | Manufacturing | 2022 |
| Stephen Craig Limited | Professional & scientific | 2002 |
| Stepwise Mobility Limited | Health & social care | 2026 |
| Steve Miller Solutions Ltd. | Professional & scientific | 2001 |
| Steven Croal Hairdressing Ltd. | Other services | 2008 |
| Stewart Morris Joinery Ltd | Construction | 2025 |
| Stirling Building Maintenance Limited | Construction | 1989 |
| Stirling Catering Limited | Hospitality | 2016 |
| Stirling City Centre BID Limited | Administrative & support | 2015 |
| Stirling Cleaning Services Ltd | Administrative & support | 2024 |
| Stirling Dog Behavioral Centre Ltd | Education | 2024 |