| Time to Pass Ltd | Education | 2025 |
| TIN Foods Ltd | Hospitality | 2015 |
| Tinwald Power Limited | Energy | 2013 |
| Tiny Town Collective Ltd | Hospitality | 2026 |
| Tiny Town Dumfries Ltd | Arts & recreation | 2023 |
| Tjmcproperty Ltd | Real estate | 2023 |
| TLI Dumfries Ltd | Hospitality | 2025 |
| TLM Valeting Limited | Extraterritorial | 2021 |
| TOB93 Ltd | Hospitality | 2026 |
| Todor Hristov Limited | Health & social care | 2019 |
| Tolero Holdings Limited | Real estate | 2020 |
| Tollcross Retail Ltd | Wholesale & retail | 2025 |
| Torr House Limited | Arts & recreation | 2013 |
| Torta by Creative Cakes Ltd | Manufacturing | 2025 |
| Toucan Property Holdings Limited | Real estate | 2024 |
| Town Dynamics Limited | Administrative & support | 2003 |
| Townhouse Dumfries Accommodation Ltd | Hospitality | 2021 |
| Tpegs Limited | Arts & recreation | 2012 |
| TR Sports Ltd | Wholesale & retail | 2025 |
| Tradacar Ltd | Wholesale & retail | 2019 |
| Transline Europe Limited | Transport & logistics | 2017 |
| Treasure Island Nursery Limited | Education | 2011 |
| Treasured Greetings Ltd | Wholesale & retail | 2015 |
| Tree Hugger & SON Tree Services Limited | Administrative & support | 2020 |
| Tribe Fortune Ltd | Health & social care | 2024 |
| Trigan Ltd | Information & communication | 2021 |
| Triple G Y Limited | Real estate | 2025 |
| Trixpixmedia Ltd | Agriculture & fishing | 2011 |
| Trossachs Wildlife Viewing Limited | Administrative & support | 2023 |
| Truscape Groundworks Limited | Administrative & support | 2025 |
| TU MED Limited | Health & social care | 2024 |
| Tudor Ballet Ltd | Arts & recreation | 2024 |
| Turkish Culture and Heritage C.I.C. | Education | 2021 |
| Turkish Language Schools Executive Limited | Education | 2025 |
| Turkish-Scottish Chamber of Commerce and Industry | Administrative & support | 2014 |
| UK Health Sales Limited | Wholesale & retail | 2016 |
| Ultimate Aggregates Limited | Wholesale & retail | 2024 |
| Unicorn Catering Services Limited | Manufacturing | 2006 |
| Unified Automation Ltd | Professional & scientific | 2023 |
| United Taxis Dumfries Ltd | Transport & logistics | 2026 |
| Upland Arts Development CIC | Arts & recreation | 2008 |
| Upland Drink Limited | Hospitality | 2024 |
| Upper Stepford Farm Ltd | Hospitality | 2019 |
| Uteachmee Ltd | Education | 2025 |
| Utility HUT Ltd | Administrative & support | 2020 |
| Vajrasattva Kadampa Meditation Centre | Education | 1993 |
| Vandom LCV Ltd | Wholesale & retail | 2025 |
| Vanity Rose Ltd | Other services | 2018 |
| Vavuniya Retail Ltd | Wholesale & retail | 2025 |
| Velo Montgomerie Limited | Manufacturing | 2014 |
| Veltrix Systems Ltd | Professional & scientific | 2025 |
| VENTURE4 Telecoms Systems Ltd | Extraterritorial | 2017 |
| Veristance Ltd | Extraterritorial | 2021 |
| Vertical Feet Limited | Real estate | 2016 |
| Vigilant Proception Limited | Extraterritorial | 2021 |
| Vigilant Protect UK Limited | Administrative & support | 2000 |
| VIP Tanning KD Ltd | Other services | 2023 |
| Virtue Recruitment Services Ltd | Administrative & support | 2015 |
| VP Golf Limited | Arts & recreation | 2018 |
| Vulture Recycling Equipment Limited | Water & waste | 2022 |
| W&S (Nominees) Limited | Professional & scientific | 2007 |
| W. H. Mcwilliam Limited | Transport & logistics | 2008 |
| W. Johnstone & Sons Limited | Construction | 2003 |
| W. W. & S. Limited | Hospitality | 2006 |
| W.L.& W. Peacock (Truck Services) Limited | Wholesale & retail | 1971 |
| Walc Holdings Limited | Administrative & support | 2020 |
| Walls & Wilde Ltd | Hospitality | 2025 |
| Waltons Consultancy Ltd | Professional & scientific | 2019 |
| Waltons Hospitality Ltd | Hospitality | 2022 |
| Waltons Property Ltd | Real estate | 2025 |
| Warwick's Newsagents Limited | Wholesale & retail | 2023 |
| Waterfront Takeaway Limited | Hospitality | 2023 |
| WCV Ltd | Wholesale & retail | 2016 |
| WE ARE Eident Limited | Manufacturing | 2026 |
| WE Love Property (Sales) Limited | Real estate | 2025 |
| WE the Bees Ltd | Manufacturing | 2025 |
| Web Focus Ltd | Professional & scientific | 2024 |
| Web Forestry Ltd | Agriculture & fishing | 2019 |
| WEE COG Ltd | Information & communication | 2017 |
| Weldtek Ltd | Professional & scientific | 2024 |
| Welldale Mill Developments Limited | Administrative & support | 2020 |
| Wellness Centre Dumfries Limited | Administrative & support | 2018 |
| Wells Builders Ltd | Construction | 2025 |
| Wellsprings Counselling Ltd | Health & social care | 2025 |
| West Coast Engineering Ltd | Professional & scientific | 2019 |
| West Coast Groundworks and Construction Limited | Construction | 2020 |
| Westlands Country Park Limited | Arts & recreation | 2015 |
| WH S 1 Ltd | Financial services | 2020 |
| Whinnyhall S Limited | Real estate | 2020 |
| Whisky Globetrotters Ltd | Information & communication | 2025 |
| Whiskymac Scotland Limited | Information & communication | 2000 |
| Whistlestop Cafe (Dumfries) Limited | Hospitality | 2020 |
| Whitesand Chop Limited | Hospitality | 2024 |
| Whiteside Farm Limited Partnership | | 1988 |
| Wild Boar Ltd | Real estate | 2013 |
| Wildlifecam Ltd | Wholesale & retail | 2022 |
| Wildwoods & Forestry Ltd | Agriculture & fishing | 2022 |
| William Clark & SON (Parkgate) Limited | Manufacturing | 1970 |
| Willie's Wraps & Rolls Ltd | Hospitality | 2019 |
| Willson Services Limited | Wholesale & retail | 2003 |