| Sypland Glen Eggs Limited | Wholesale & retail | 2024 |
| T and K Retail Ltd | Wholesale & retail | 2024 |
| T. Mckie Limited | Construction | 2003 |
| T.B. Watson Limited | Wholesale & retail | 1924 |
| Tacktrim Limited | Professional & scientific | 1984 |
| Take the Pressure OFF Ltd | Administrative & support | 2026 |
| Tantazia Ltd | Other services | 2023 |
| Tantrum Events Ltd | Wholesale & retail | 2023 |
| Tartan Hens Ltd | Arts & recreation | 2024 |
| TAS Building Supplies Limited | Wholesale & retail | 2008 |
| Tattersfield Properties Limited | Households | 2018 |
| Taylor Made Fabrication Services Limited | Manufacturing | 2024 |
| TBC (West) Ltd | Hospitality | 2018 |
| TC Enterprises Dumfries Ltd | Hospitality | 2026 |
| Techsol Scot Ltd | Other services | 2019 |
| Telfer Hope Limited | Households | 2018 |
| Telford Managing Agent Co Ltd | Administrative & support | 2012 |
| Temple Brush Co Limited | Construction | 2025 |
| Templetons Cookies Limited | Wholesale & retail | 2024 |
| Terraughtie Farming Company Limited | Agriculture & fishing | 1965 |
| TH Projects Ltd | Extraterritorial | 2011 |
| THE Alexander Rural Initiative Limited | Professional & scientific | 2017 |
| THE Auld Kirk Ltd | Construction | 2025 |
| THE Back Street Restaurant Ltd | Hospitality | 2019 |
| THE Balgray and Raehills Shoot Limited | Arts & recreation | 2021 |
| THE Barbuie Farm Partnership | | 1999 |
| THE Barkin Barber Limited | Professional & scientific | 2023 |
| THE Black Grouse (Scotland) Ltd | Real estate | 2021 |
| THE Bonnie Laundry Ltd | Other services | 2026 |
| THE Buccleuch Limited | Hospitality | 2013 |
| THE Care Shop (Dumfries) Limited | Wholesale & retail | 2010 |
| THE Castle Beauty Salon Limited | Other services | 2014 |
| THE Chipsmyth Ltd | Hospitality | 2015 |
| THE Crichton Trust | Real estate | 1996 |
| THE Dollz House Dumfries Ltd | Other services | 2024 |
| THE Doonhamer Ltd | Hospitality | 2026 |
| THE Earth's Crust Bakery Limited | Manufacturing | 2012 |
| THE Eco Group (Scotland) Ltd | Construction | 2024 |
| THE Edinburgh Piano Company Limited | Wholesale & retail | 1997 |
| THE Eriskay Pony Society Limited | Agriculture & fishing | 1995 |
| THE Famous Star Hotel Limited | Hospitality | 2014 |
| THE Food Train Limited | Health & social care | 1995 |
| THE Grain Store Dumfries Limited | Real estate | 2021 |
| THE Great British Podiatry Clinic Ltd | Health & social care | 2023 |
| THE Home and Floor Store Dumfries Ltd | Wholesale & retail | 2019 |
| THE Horse Scene UK Limited | Agriculture & fishing | 2025 |
| THE India Palm Ltd | Hospitality | 2018 |
| THE Jewel in the Crown (Dumfries) Limited | Hospitality | 2014 |
| THE Leaf and OIL Company Ltd | Wholesale & retail | 2015 |
| THE Learning Lounge (Dumfries) Ltd | Education | 2025 |
| THE Little Bakery (Kirkcudbright) Limited | Wholesale & retail | 2019 |
| THE Marina Hotel (Mckeown) Limited | Hospitality | 2021 |
| THE Market Place DG1 Ltd | Wholesale & retail | 2026 |
| THE New Steamboat Limited | Hospitality | 2013 |
| THE Normandy Bar Limited | Hospitality | 2019 |
| THE Opalite Clinic Limited | Other services | 2026 |
| THE Open Arms Dumfries CIC | Arts & recreation | 2026 |
| THE POSTHORN90 Annan Ltd | Wholesale & retail | 2016 |
| THE RAP Foundation | Administrative & support | 2002 |
| THE Redhills Farming Company | | 1998 |
| THE Redundancy Lady Ltd | Professional & scientific | 2023 |
| THE Roussin Sheep Society of the British Isles Ltd. | Agriculture & fishing | 1989 |
| THE Safe Road User Academy Ltd | Education | 2025 |
| THE Safe Sport Hub Ltd | Information & communication | 2026 |
| THE Salon @ Barbours Ltd | Other services | 2023 |
| THE Sample Guys Limited | Wholesale & retail | 2021 |
| THE Shoe Shop (Dumfries) Limited | Wholesale & retail | 2019 |
| THE Shop Dumfries Limited | Wholesale & retail | 2025 |
| THE Soccer Hub Ltd | Arts & recreation | 2026 |
| THE Solway Firth Partnership | Professional & scientific | 2003 |
| THE Southern Uplands Partnership | Professional & scientific | 1999 |
| THE Southwest Community Woodlands Trust | Education | 1997 |
| THE Specialist Driving School Limited | Education | 2022 |
| THE Stove Network (Trading) Limited | Arts & recreation | 2011 |
| THE Stove Network Limited | Arts & recreation | 2011 |
| THE Swan at Kingholm Ltd. | Hospitality | 2003 |
| THE Swan at the Quay Limited | Hospitality | 2026 |
| THE Tech Repair Centre Ltd | Other services | 2019 |
| THE Townhouse Hotel Dumfries Ltd | Hospitality | 2019 |
| THE Training Team (Scotland) Limited | Education | 2016 |
| THE Tuff Tipper Limited | Transport & logistics | 2015 |
| THE Waterfront (Dumfries) Ltd | Hospitality | 2026 |
| THE Wedding Room Dumfries Ltd | Administrative & support | 2020 |
| THE Wight House Aesthetics Limited | Other services | 2026 |
| THE Wily Rhino Ltd | Information & communication | 2021 |
| Think Something Different Dumfries Limited | Wholesale & retail | 2019 |
| Third Farming Partners LLP | | 2025 |
| Thomas Barker (Old Irvine) & Co | | 2000 |
| Thomas Cairnie & SON Limited | Wholesale & retail | 2004 |
| Thomson Foodservice Limited | Wholesale & retail | 2017 |
| Thomson Heating & GAS Services Ltd | Construction | 2021 |
| Thomson Mortgages Limited | Financial services | 2004 |
| Thomson Real Estate Ltd | Real estate | 2025 |
| Thomson Roddick Callan Limited | Administrative & support | 2022 |
| Thomson Roddick Scottish Auctions Limited | Professional & scientific | 2009 |
| Thuinn Subsea Limited | Professional & scientific | 2026 |
| Tighnabruaich Boatyard Limited | Manufacturing | 2020 |
| Tighnabruaich Enterprises Limited | Financial services | 2020 |
| Tighnabruaich Moorings Limited | Arts & recreation | 2020 |
| Timber Trading Post Limited | Agriculture & fishing | 2002 |