| 105 Comms Ltd | Wholesale & retail | 2018 |
| 12 Tasks Ltd | Information & communication | 2019 |
| 19TH Hole (Southerness) Limited | Hospitality | 2021 |
| 22 Energy Systems Ltd | Construction | 2025 |
| 24S Property Rentals Limited | Real estate | 2025 |
| 27 Cars Ltd | Transport & logistics | 2018 |
| 3 Glow Bears Ltd | Education | 2024 |
| 360 Asset Holdings Ltd | Financial services | 2025 |
| 360 Degree Construction Limited | Construction | 2026 |
| 360 Degree Flooring Limited | Construction | 2017 |
| 4K Property Ltd | Real estate | 2023 |
| 509 Ltd | Real estate | 2024 |
| 6TY Shades of Beauty (Dumfries) Limited | Other services | 2018 |
| A & a Henderson Ltd | Agriculture & fishing | 2025 |
| A & a Nicholson Limited | Agriculture & fishing | 2006 |
| A & B Inn Ltd | Hospitality | 2026 |
| A & CM Engineering Limited | Construction | 2024 |
| A & E Healthcare Ltd | Manufacturing | 2012 |
| A & G Homes (Scotland) Limited | Construction | 2025 |
| A & H Floor Covering Specialists Limited | Construction | 2002 |
| A & M Chemicals Ltd | Wholesale & retail | 2026 |
| A Armstrong Electricians Ltd | Construction | 2010 |
| A Houston Builders Limited | Construction | 2024 |
| A M Plumbing Limited | Construction | 2004 |
| A M POW Marine Services Ltd | Administrative & support | 2022 |
| A Mcnay Contracting Ltd | Other services | 2023 |
| A. & B. Clement Limited | Wholesale & retail | 1995 |
| A. Mccornick & Co. (Midcroft) | | 2000 |
| A.I Engineering Ltd | Manufacturing | 2010 |
| A.Quine (Investments) Limited | Real estate | 2005 |
| Aaronova Limited | Wholesale & retail | 2025 |
| Aathan UK Properties Ltd | Real estate | 2025 |
| Aatheip Retail Ltd | Wholesale & retail | 2021 |
| AB Global Mart Ltd | Wholesale & retail | 2026 |
| Abbas Prop Ltd | Real estate | 2026 |
| Abbey Croft Associates Limited | Professional & scientific | 2016 |
| Abbey Supplies Limited | Other services | 2008 |
| Absolute Vitamins Limited | Wholesale & retail | 2023 |
| Academy Street Consulting Ltd | Professional & scientific | 2024 |
| Accounts@Donneers Limited | Professional & scientific | 2016 |
| Accurate Digital Pathology Limited | Professional & scientific | 2025 |
| Acorn Glen Ltd | Other services | 2019 |
| AD Contracting Limited | Agriculture & fishing | 2017 |
| AD Restore Ltd | Information & communication | 2024 |
| Adamira Driving School Ltd | Education | 2025 |
| ADH AD Limited | Energy | 2016 |
| Adiona Commercial Vehicles Ltd | Wholesale & retail | 2018 |
| ADS Fire Services Limited | Manufacturing | 2012 |
| Advice for Life Holdings Limited | Financial services | 2021 |
| AE Auto Service Limited | Wholesale & retail | 2025 |
| AE Bike Shop Limited | Wholesale & retail | 2013 |
| Aedai (AE Design and Install) Ltd | Construction | 2021 |
| Aegir SRA Limited | Professional & scientific | 2010 |
| Affinity Projects 131 Limited | Construction | 2023 |
| AFL Financial Planning Services Limited | Professional & scientific | 2016 |
| Agileshop Ltd | Wholesale & retail | 2022 |
| Agora Gardens Ltd | Agriculture & fishing | 2021 |
| Agriwheel Limited | Professional & scientific | 2021 |
| AGW Services Ltd | Professional & scientific | 2021 |
| AI Designworks Ltd | Professional & scientific | 2024 |
| Ailsa Films Limited | Professional & scientific | 2019 |
| Aj&Bj Property Ltd | Real estate | 2024 |
| AJG Trading (Lockerbie) Limited | Manufacturing | 2024 |
| AK Building (Scotland) Limited | Construction | 2023 |
| Alan Kirk Plumbing & Heating Ltd | Construction | 2016 |
| Alan Young Engineering Holdings Limited | Financial services | 2020 |
| Alan Young Engineering Limited | Manufacturing | 1992 |
| Albatec Racing Limited | Manufacturing | 2012 |
| Albatec Sport Limited | Arts & recreation | 2012 |
| ALC Services (Scotland) Limited | Water & waste | 2016 |
| Alcohol and Drugs Support South West Scotland | Health & social care | 2002 |
| Alegio Limited | Extraterritorial | 2022 |
| Aletheia Architecture Ltd | Information & communication | 2026 |
| Algidus Holdings Ltd | Agriculture & fishing | 2025 |
| Alison Mcwhirter Ltd | Arts & recreation | 2017 |
| ALL Blacks Property Services Limited | Construction | 2025 |
| Allan Smith Fencing & Forestry Limited | Agriculture & fishing | 2005 |
| Allthingspool Limited | Wholesale & retail | 2016 |
| Almagill Farming Partners LLP | | 2024 |
| Alpha Powder Coating & Exhausts Ltd | Manufacturing | 2020 |
| Alsace International Ltd | Wholesale & retail | 2023 |
| Alteracore Ltd | Information & communication | 2025 |
| Always Ready Club Limited | Arts & recreation | 2024 |
| Always Ready Fit Box Limited | Arts & recreation | 2024 |
| Alyalais Protected Falcon Limited | Agriculture & fishing | 2017 |
| AM Properties Scotland Ltd | Real estate | 2024 |
| AMB Engineering (Dumfries) Ltd | Professional & scientific | 2011 |
| Amberlux Ltd | Wholesale & retail | 2021 |
| Ambox Packaging Ltd | Wholesale & retail | 2025 |
| AMD Contract Services Limited | Administrative & support | 2008 |
| AMD Plant Hire Ltd | Administrative & support | 2012 |
| AML Haulage Limited | Transport & logistics | 2015 |
| Amsbras Ltd | Professional & scientific | 2025 |
| Amy's Oak Tree Ltd | Health & social care | 2025 |
| Anayah Catering Limited | Hospitality | 2023 |
| Anderson Fire Consultancy Ltd | Public administration | 2022 |
| Anderson Kilts Ltd | Wholesale & retail | 2007 |
| Andrew J. Ross Limited | Construction | 2002 |
| Andrew R. Wilson Limited | Wholesale & retail | 1984 |
| Andy Goldsworthy Ltd | Extraterritorial | 2014 |