| Andy Thom Plumbing & Heating Limited | Construction | 2018 |
| Animals Supporting People at Fred C.I.C. | Other services | 2017 |
| Anman Limited | Real estate | 2003 |
| Annan Travel Limited | Administrative & support | 2013 |
| Annandale Auto-Services (Collin) Limited | | 1966 |
| Annandale Financial Services LLP | | 2012 |
| Annandale Safety and Compliance Ltd | Professional & scientific | 2020 |
| Aoraki & Co Limited | Hospitality | 2023 |
| AP Stonemasonry & Building Services Ltd | Manufacturing | 2026 |
| Apex Wind Ltd | Manufacturing | 2025 |
| APH Travel Services Ltd | Administrative & support | 2026 |
| Applied Care and Development Limited | Health & social care | 2001 |
| APS Contractors Group Limited | Construction | 2023 |
| Arbigland House & Gardens Limited | Real estate | 2018 |
| Arcane Workshop Limited | Information & communication | 2014 |
| Archangel Security and Consultancy Limited | Other services | 2022 |
| Archive Technical Services Limited | Professional & scientific | 2023 |
| Ariyapot Catering Services Limited | Hospitality | 2025 |
| ARK VET Centre Limited | Professional & scientific | 2012 |
| Arms Around Mabie Ltd | Hospitality | 2025 |
| Armstrong Brothers Holdings Limited | Financial services | 2024 |
| Armstrong Brothers Limited | Wholesale & retail | 2021 |
| Armstrong E&I Services Ltd | Construction | 2023 |
| Armstrong Group (Scotland) Limited | Professional & scientific | 2017 |
| Armstrong Properties (Scotland) Limited | Real estate | 2000 |
| Armstrong Renewables (Scotland) Limited | Water & waste | 2017 |
| Armstrong Trucks Limited | Wholesale & retail | 2006 |
| Armstrong Waste Management Limited | Water & waste | 1988 |
| Arokas Shop Dumfries Limited | Wholesale & retail | 2025 |
| Arranview Manor Ltd | Hospitality | 2019 |
| Artel Associates Limited | Professional & scientific | 2011 |
| Artsd&G | Arts & recreation | 1997 |
| AS Foods Dumfries Ltd | Wholesale & retail | 2024 |
| ASD GAS Services Ltd | Construction | 2023 |
| ASH & Vine Ltd | Manufacturing | 2022 |
| Asher Associates Limited | Professional & scientific | 1998 |
| Asian Flavours Newcastle Ltd | Hospitality | 2022 |
| Asquared Consultancy (Pharma) Limited | Professional & scientific | 2025 |
| Association of Trail Builders | Education | 2020 |
| Astrid Orth Limited | Health & social care | 2012 |
| Asur Body Centre CIC | Health & social care | 2024 |
| Atkin Property Ltd | Agriculture & fishing | 2022 |
| Atlas Maintenance (ALL Trades) Limited | Administrative & support | 1995 |
| Atlas RX Ltd | Health & social care | 2025 |
| Auchengray Farms | | 1982 |
| Auchensala Genetics Limited | Administrative & support | 2025 |
| Auchnane Consultancy Limited | Professional & scientific | 2019 |
| Auld Skool Events Ltd | Arts & recreation | 2022 |
| Austin House Limited | Real estate | 2023 |
| Auto Dental Equipment Ltd | Manufacturing | 2026 |
| Autobeam Limited | Wholesale & retail | 2016 |
| Autoshop Online Limited | Wholesale & retail | 2021 |
| AVC Property Investments Limited | Real estate | 2021 |
| AVM Transport Limited | Transport & logistics | 2018 |
| AW 2.0 Limited | Information & communication | 2007 |
| Awam Ventures Ltd | Wholesale & retail | 2020 |
| AWC 2023 Ltd | Financial services | 2023 |
| Awco Developments Ltd | Real estate | 2025 |
| AXL Menlove Roofing Ltd | Construction | 2023 |
| Ayrshire Animal By=Products Limited | Manufacturing | 2022 |
| Ayrshire Care Limited | Health & social care | 2019 |
| B & H Johnstone Ltd | Agriculture & fishing | 2012 |
| B & N Robertson Limited | Wholesale & retail | 2008 |
| B & T Hotel Limited | Hospitality | 2024 |
| B and K Joinery Limited | Construction | 2018 |
| B Sure Brokers Ltd | Administrative & support | 2011 |
| B&M Kitchen Limited | Hospitality | 2024 |
| BAA Tech Ltd | Professional & scientific | 2015 |
| BAC Farming | Agriculture & fishing | 2006 |
| Baillieknowe Farmers | | 1991 |
| Bainne BÓ Limited | Agriculture & fishing | 2025 |
| Baka Chiropractic Limited | Health & social care | 2008 |
| Balcary Services Ltd | Hospitality | 2020 |
| Balker Farms Limited | Agriculture & fishing | 1986 |
| Balliol Property Services Limited | Households | 2021 |
| Balmoral Road Car Sales and Service Ltd | Wholesale & retail | 2013 |
| Baltus Limited | Wholesale & retail | 2015 |
| Banks Farmers | | 1991 |
| Baraka Halal Ltd | Wholesale & retail | 2024 |
| Bard Vets Limited | Professional & scientific | 2008 |
| Barend Bar and Restaurant Limited | Hospitality | 2011 |
| Barend Proprietors Association Limited | Administrative & support | 1973 |
| Barjarg Trees Ltd | Agriculture & fishing | 2021 |
| Barnhill Cabs Ltd | Transport & logistics | 2012 |
| Barry’S Driving School Ltd | Education | 2026 |
| Barshill Farm Ltd | Agriculture & fishing | 2022 |
| Basement Pumps Scotland Limited | Professional & scientific | 2024 |
| Bashir & Bashir Limited | Hospitality | 2022 |
| BASHIR233 Limited | Other services | 2025 |
| Bay Horse Properties Ltd | Real estate | 2020 |
| BB & Co Limited | Health & social care | 2012 |
| BDS (Dumfries) Limited | Professional & scientific | 2020 |
| Bdsdigital Ltd | Information & communication | 2024 |
| Bearcroft Farms Limited | Agriculture & fishing | 1963 |
| Beautiful Beginnings Photography Ltd | Professional & scientific | 2009 |
| Beechwood Homecare Limited | Health & social care | 2008 |
| Beer Without Borders Limited | Real estate | 2018 |
| Bell Lawrie of Biggar (Developments) Limited | Construction | 1986 |
| Bella Cures Limited | Other services | 2014 |
| Bella Estetica Limited | Health & social care | 2022 |