| 'DE Novo' Care Ltd | Health & social care | 2017 |
| 1910 Developments Limited | Construction | 2003 |
| 3D Lettings Ltd | Hospitality | 2011 |
| 5TH Generation Property Ltd | Real estate | 2022 |
| 7 Hills Mechanical Limited | Construction | 2026 |
| A & P Solutions Limited | Professional & scientific | 2003 |
| A & Z Renovations Ltd | Real estate | 2025 |
| A Line Holdings Limited | Real estate | 2020 |
| A Small World Ltd | Wholesale & retail | 2022 |
| A Strong Lettings Limited | Real estate | 2014 |
| A Strong Wealth Ltd | Other services | 2017 |
| A.P.T. Financial Planning Limited | Financial services | 2013 |
| A.S.H. Business Growth Ltd | Professional & scientific | 2021 |
| A61 Woodseats Sewing Machines Limited | Wholesale & retail | 1965 |
| ABN Health Ltd | Health & social care | 2026 |
| Accessible Group Limited | Professional & scientific | 2022 |
| ACCOUNTABLE4U (Chesterfield) Ltd | Professional & scientific | 2022 |
| ACW Medical Service Ltd | Health & social care | 2021 |
| ADD 5019 Ltd | Extraterritorial | 2015 |
| Advanced Fabrication and Engineering Ltd | Manufacturing | 2026 |
| Advanced Testing Services Ltd | Professional & scientific | 2026 |
| Advanced Testing Solutions Limited | Information & communication | 2015 |
| Aesthetics by RIO Limited | Health & social care | 2023 |
| AET Flood Defence Limited | Construction | 2013 |
| AF Engineering Consultancy Ltd | Professional & scientific | 2025 |
| AFA Contracts Ltd | Construction | 2024 |
| AG Creative Bids Consultancy Ltd | Professional & scientific | 2023 |
| Agile Minds Coaching Ltd | Professional & scientific | 2024 |
| AI Motorhouse Ltd | Wholesale & retail | 2025 |
| AI Property Partners Ltd | Hospitality | 2025 |
| Aimee Thompson Transport Planning Limited | Professional & scientific | 2018 |
| Ainsworth Kitchens & ALE Limited | Hospitality | 2019 |
| AJ Sheffield Taxi Ltd | Transport & logistics | 2022 |
| AJD Services UK Ltd | Information & communication | 2025 |
| AJS Diagnostics Limited | Wholesale & retail | 2011 |
| AJS Financial Management Ltd | Financial services | 2017 |
| AJW Accounts Limited | Professional & scientific | 2024 |
| Akademy Football Pitches Limited | Arts & recreation | 2021 |
| AL Plus Ltd | Other services | 2024 |
| Alaya Ltd | Health & social care | 2018 |
| Alex Bacon Ltd | Professional & scientific | 2017 |
| Alexander Nicolle Limited | Professional & scientific | 2011 |
| Alfred Dunham & SON Limited | Wholesale & retail | 1976 |
| ALH PUB Services Ltd | Professional & scientific | 2024 |
| Align Creative Services Limited | Administrative & support | 2007 |
| ALL Seasons Chimney Services Ltd | Administrative & support | 2022 |
| ALL Things Nice Ltd | Wholesale & retail | 2024 |
| Alliance Alloys Ltd | Wholesale & retail | 2024 |
| Alton Estates Limited | Construction | 2010 |
| Aluminium Projects Ltd | Other services | 2016 |
| Amare Hair & Beauty Limited | Other services | 2022 |
| AME Elec Ltd | Construction | 2024 |
| AME Properties (North) Ltd | Real estate | 2025 |
| American Chrome Limited | Wholesale & retail | 2013 |
| Amoll Limited | Administrative & support | 2017 |
| Anaco Trading Limited | Wholesale & retail | 1998 |
| ANG Theatre Schools Ltd | Arts & recreation | 2024 |
| Apex Fibre Ltd | Information & communication | 2026 |
| Apollo Appliances Ltd | Wholesale & retail | 2008 |
| Apollo Pest Control & Hygiene Services Limited | Administrative & support | 2018 |
| AR Jones Holdings Ltd | Financial services | 2023 |
| Arborits Groundcare Services Limited | Administrative & support | 2015 |
| Arbs Dronfield Ltd | Construction | 2024 |
| Arcadia EPR Solutions Ltd | Professional & scientific | 2026 |
| Archimedes Earth Limited | Education | 2002 |
| Archimedes Global Limited | Real estate | 2020 |
| Arena Netball C.I.C. | Arts & recreation | 2022 |
| Armeg EOT Limited | Financial services | 2024 |
| Armeg Holdings Limited | Professional & scientific | 2017 |
| Aromaxy Limited | Wholesale & retail | 2025 |
| Art & Associates Ltd | Real estate | 2017 |
| Ascena Products Limited | Manufacturing | 2015 |
| Ascento Trustee Limited | Extraterritorial | 2021 |
| ASD Estates Ltd | Real estate | 2021 |
| Ashford Support Services Ltd | Administrative & support | 2025 |
| Ashwood Homes (Derbyshire) Limited | Construction | 2007 |
| Asmet (UK) Limited | Wholesale & retail | 1993 |
| Asmet Limited | Financial services | 2009 |
| Asset Property Care UK Ltd | Real estate | 2026 |
| AT Comms Consulting Ltd | Information & communication | 2023 |
| Atomic Industries Limited | Construction | 2012 |
| Atrium Dronfield Ltd | Hospitality | 2018 |
| AUM Dent Group 2 Ltd | Health & social care | 2026 |
| Auriga Automotive Limited | Wholesale & retail | 2004 |
| Aviation Data Services Ltd | Information & communication | 2014 |
| AW Central Ltd | Information & communication | 2025 |
| Axenergi Consultancy Limited | Professional & scientific | 2023 |
| B R Hoult Ltd | Hospitality | 2018 |
| B Realestates Ltd | Construction | 2013 |
| B&E Properties Ltd | Real estate | 2025 |
| B3 Wealth Management Limited | Financial services | 2007 |
| BA Housekeeping Ltd | Administrative & support | 2022 |
| Bamboo (Dronfield) Hair & Beauty Limited | Other services | 2016 |
| Bar-Belle's Ltd | Arts & recreation | 2025 |
| Barker Agar Electrical Limited | Construction | 2021 |
| Barrow Roofing Limited | Construction | 2002 |
| Bashmoor Limited | Real estate | 1982 |
| Beach Schools Ltd | Education | 2013 |
| Beacon Hill Point Holdings Limited | Financial services | 2026 |
| Beacon Hill Point RES 1 Ltd | Real estate | 2026 |