| SCL Sports Limited | Professional & scientific | 2024 |
| Scooter Solutions Limited | Wholesale & retail | 2023 |
| Scubado Ltd | Arts & recreation | 2022 |
| SCY Technologies Limited | Health & social care | 2025 |
| Scytronix Ltd | Professional & scientific | 2010 |
| Scytronix Systems Ltd | Professional & scientific | 2022 |
| SDW Outsource Limited | Professional & scientific | 2016 |
| SE Ents Limited | Arts & recreation | 2012 |
| Sedman Shoes Limited | Wholesale & retail | 2009 |
| Select Development Yorkshire Ltd | Construction | 2020 |
| Severals Holdings Ltd | Financial services | 2024 |
| SGH (Hull) Limited | Transport & logistics | 2017 |
| Shanley Group Ltd | Financial services | 2026 |
| Sheep Rake Consultancy Ltd | Agriculture & fishing | 2025 |
| Shepcote Distributors Limited | Manufacturing | 1969 |
| Shepcote Holdings Limited | Real estate | 1975 |
| Shepherds Croft Management Company Limited | Other services | 2019 |
| Sherlock Homes Hull Ltd | Real estate | 2020 |
| Shohel's ZL Limited | Hospitality | 2024 |
| Shore HR Limited | Administrative & support | 2024 |
| Shore Solutions Ltd | Information & communication | 2026 |
| Short Neil 1263 Limited | Transport & logistics | 2014 |
| Simeon Halstead + Associates Limited | Professional & scientific | 2014 |
| Simply Shuttered Ltd | Extraterritorial | 2019 |
| Sincerity Home Care Services Limited | Health & social care | 2017 |
| Sirr Property Services Limited | Real estate | 2019 |
| Sitwell Court Management Company Limited | Households | 1991 |
| SJH Properties Yorkshire Limited | Real estate | 2025 |
| Sjkj Holdings Ltd | Financial services | 2025 |
| Skerne Park Investments Limited | Construction | 2024 |
| Skerne Road Developments Limited | Construction | 2012 |
| Skipsea Caravan Windows Ltd | Construction | 2013 |
| Skipsea Service Station Ltd | Manufacturing | 2015 |
| SKJ Property Services (Scarborough) Limited | Real estate | 2017 |
| Skot Holdings Limited | Real estate | 2017 |
| Skot Management Limited | Professional & scientific | 2021 |
| Sky Magic Events Ltd | Arts & recreation | 2020 |
| Sky Nail & Spa Ltd | Other services | 2025 |
| Skynmjk Limited | Wholesale & retail | 2026 |
| Sledmere Estate Management Company Limited | Professional & scientific | 2020 |
| Sleightholme Farms Limited | Agriculture & fishing | 2018 |
| SLM Yorkshire Limited | Real estate | 2026 |
| Sloe Trading Ltd | Wholesale & retail | 2024 |
| SM Sporting Limited | Arts & recreation | 2023 |
| Smart-Flo Ltd | Construction | 2010 |
| Smith Agricultural Services Ltd | Wholesale & retail | 2025 |
| Smiths Handyman Services Ltd | Construction | 2021 |
| Snowden Farm Services Limited | Agriculture & fishing | 2014 |
| Soanes Poultry Ltd | Manufacturing | 2004 |
| SOL Interiors Limited | Construction | 2005 |
| SOL Specialist Assets Limited | Administrative & support | 2016 |
| Solacycles Ltd | Wholesale & retail | 2018 |
| Soper Holdings Limited | Professional & scientific | 2006 |
| Sophie’S Deals Ltd | Professional & scientific | 2026 |
| South Liverpool Interiors Ltd | Construction | 2023 |
| Southern Seauxe Consulting Ltd | Professional & scientific | 2020 |
| Southfield Agronomy Limited | Agriculture & fishing | 2009 |
| Sparkling Touches Limited | Extraterritorial | 2004 |
| Specialist Home Developments Limited | Construction | 2005 |
| Speedway Couriers Ltd | Transport & logistics | 2006 |
| Spence Farms Limited | Agriculture & fishing | 2022 |
| Spenergy Limited | Energy | 2012 |
| Sperry Tents UK South West Limited | Administrative & support | 2015 |
| Spirit of Yorkshire Limited | Manufacturing | 2012 |
| Splav Limited | Real estate | 2010 |
| SPM (Hull) Limited | Households | 2021 |
| Spot-On Property Maintenance Limited | Other services | 2022 |
| Spring Road News Limited | Wholesale & retail | 2016 |
| Spud Agronomy & Consultancy Limited | Professional & scientific | 2011 |
| Spuds & Berries Ltd | Hospitality | 2016 |
| Square Cactus Limited | Information & communication | 2015 |
| Squash Limited | Professional & scientific | 2003 |
| ST George's Keep (York) Ltd | Hospitality | 2011 |
| ST James Warter Preservation Trust | Arts & recreation | 1996 |
| ST Plumbing and Bathrooms Ltd | Construction | 2026 |
| ST Plumbing CIC | Construction | 2026 |
| ST Quintin's Creamery Ltd | Agriculture & fishing | 2008 |
| Stabler Farming Limited | Agriculture & fishing | 2025 |
| Stafford UK Limited | Hospitality | 2008 |
| STAGE4BEVERLEY Ltd | Arts & recreation | 2016 |
| Stamp Brothers Ltd | Administrative & support | 2024 |
| Stand Your Ground Companion CIC | Public administration | 2024 |
| Stanley Marshall (Farms) Limited | Agriculture & fishing | 1963 |
| Stbh (UK) Ltd | Wholesale & retail | 2019 |
| Stellar Statics Limited | Manufacturing | 2004 |
| Stephen B Wells Limited | Agriculture & fishing | 2004 |
| Steve Wright (Poultry) Limited | Agriculture & fishing | 2004 |
| Steven Sharpley Transport Limited | Transport & logistics | 2015 |
| Stones Business Services Ltd | Administrative & support | 2018 |
| Stones Wealth Limited | Professional & scientific | 2020 |
| Stringers Whitby Limited | Wholesale & retail | 2009 |
| Structure Services (Hull) Limited | Construction | 2014 |
| SUE Dickinson Mortgages Limited | Financial services | 2015 |
| Sugarbread Fisher Limited | Real estate | 2017 |
| Sulair Holdings Limited | Administrative & support | 1986 |
| Sulair Services Limited | Extraterritorial | 1984 |
| Summit Farm Ltd | Financial services | 2025 |
| Sumo Footwear Limited | Wholesale & retail | 2005 |
| Sumo Technologies Limited | Information & communication | 2000 |
| Supergrass Forage Limited | Wholesale & retail | 2020 |