| Envelope Fixing Systems Limited | Construction | 2022 |
| Enviro Electronics Ltd | Water & waste | 2011 |
| Environconsult LLP | | 2008 |
| Environmental Electronics Ltd | Water & waste | 2020 |
| Environmental Plumbing & Heating Ltd | Construction | 2019 |
| Environmental Speciality Chemicals Limited | Manufacturing | 1998 |
| Envisage Digital Media Limited | Information & communication | 2009 |
| Envy Dental Ltd | Health & social care | 2025 |
| Envy Drive Ltd | Construction | 2023 |
| Eodsmith Ltd | Hospitality | 2026 |
| Eofjle Services Ltd | Health & social care | 2018 |
| EP Nesture Ltd | Real estate | 2025 |
| EP Operations Limited | Real estate | 2022 |
| EP Solutions Holdings Ltd | Financial services | 2025 |
| EPG Roofing Limited | Construction | 2010 |
| EPH Plumbing and Heating Ltd | Construction | 2023 |
| EPI Service Accommodation Limited | Hospitality | 2023 |
| Epicureanway.Com Ltd | Hospitality | 2025 |
| EPM Psychology Limited | Health & social care | 2026 |
| Epworth & District Agricultural Society Limited | Other services | 1996 |
| Epworth Fields Limited | Hospitality | 2021 |
| Epworth Groundworks Ltd | Construction | 2009 |
| Epworth Literary and Arts Festival Limited | Administrative & support | 2026 |
| Epworth Service Centre Ltd | Wholesale & retail | 2019 |
| EQ Media Ltd | Information & communication | 2026 |
| Equ-I-Nimity Hooves of Healing Ltd | Arts & recreation | 2026 |
| Equal Equality CIC | Manufacturing | 2025 |
| Equalives Ltd | Health & social care | 2023 |
| Equest Horseboxes Ltd | Manufacturing | 2021 |
| Equestrian Fibres Limited | Construction | 2019 |
| Equestrian Professionals Unite Ltd | Professional & scientific | 2021 |
| Equine Essential Services Limited | Agriculture & fishing | 2020 |
| Equinox Innovations Ltd | Professional & scientific | 2011 |
| Equinox Transport Ltd | Transport & logistics | 2023 |
| Equirug Ltd | Agriculture & fishing | 2025 |
| Equitable Private Midwifery Services Limited | Health & social care | 2025 |
| Equity Report Ltd | Information & communication | 2022 |
| ER CRM Ltd | Information & communication | 2025 |
| ER Doncaster Ltd | Hospitality | 2020 |
| ER Support Ltd | Professional & scientific | 2016 |
| Er&Sa Ltd | Hospitality | 2023 |
| ERA Blade Repair Services Ltd | Construction | 2026 |
| Ercan Transport Ltd | Transport & logistics | 2020 |
| Ercg Limited | Real estate | 2026 |
| Erdogdu Limited | Hospitality | 2019 |
| Eremost Limited | Real estate | 1977 |
| Eren Hill Ltd | Hospitality | 2026 |
| Erenler Takeaway Limited | Hospitality | 2025 |
| Ergobud Ltd | Construction | 2024 |
| ERH Agri Limited | Wholesale & retail | 2013 |
| Eric Andrei Delivery Ltd | Transport & logistics | 2024 |
| Eric Nicolas Delivery Ltd | Transport & logistics | 2025 |
| Eric Taylor Machinery Limited | Administrative & support | 2016 |
| Erika-Vanessa Limited | Transport & logistics | 2017 |
| ERM Transport Ltd | Transport & logistics | 2025 |
| Ernest Holdings Limited | Financial services | 2021 |
| Erni Transport Ltd | Transport & logistics | 2016 |
| Erog QP Ltd | Professional & scientific | 2022 |
| Eroom Properties Ltd | Real estate | 2021 |
| Erpsirius Limited | Information & communication | 2018 |
| Errigal Exports Ltd | Wholesale & retail | 2012 |
| Ershop Limited | Wholesale & retail | 2025 |
| Ersinrs Ltd | Transport & logistics | 2022 |
| ES Paradis Limited | Other services | 2017 |
| ESA Tyres Ltd | Wholesale & retail | 2021 |
| Esam Naji Trading Ltd | Extraterritorial | 2025 |
| ESC Global Limited | Manufacturing | 2009 |
| ESC Investments Limited | Real estate | 2010 |
| ESC Properties Limited | Real estate | 2017 |
| Esco (UK) Holdings Limited | Professional & scientific | 2003 |
| Esco (UK) Limited | Manufacturing | 1982 |
| Esco Emea Holdings (UK) Limited | Professional & scientific | 2013 |
| Esco Hydra (UK) Limited | Manufacturing | 2000 |
| Escort Logistics Ltd | Transport & logistics | 2025 |
| Esdee Associates Ltd | Professional & scientific | 2010 |
| Eseon Limited | Administrative & support | 2001 |
| ESG Trans Ltd | Transport & logistics | 2025 |
| ESH Property Developments Ltd | Construction | 2026 |
| Eshmaelmakondohealthcare Ltd | Health & social care | 2019 |
| Eskholme Property Limited | Real estate | 2023 |
| Eskiyasipinar Limited | Hospitality | 2018 |
| Esperrazza Limited | Other services | 2023 |
| Espire Hair and Beauty Ltd | Health & social care | 2020 |
| Espresso Repair Limited | Manufacturing | 2009 |
| Esquires Wheatley Limited | Hospitality | 2009 |
| ESR Property Limited | Real estate | 2017 |
| Esra Barber Limited | Other services | 2025 |
| ESS Property Holdings Limited | Financial services | 2025 |
| ESS Property Investments Limited | Real estate | 2025 |
| ESS Training Ltd | Administrative & support | 2020 |
| Essence Accounting Ltd | Professional & scientific | 2024 |
| Essential Protection of Data Ltd | Information & communication | 2016 |
| Essentialdx Limited | Wholesale & retail | 2026 |
| Essentially Energy Limited | Energy | 2025 |
| Esseservice Ltd | Professional & scientific | 2010 |
| Essor One Limited | Education | 2022 |
| Estate Hunter UK Ltd | Real estate | 2021 |
| Este Beauty Limited | Other services | 2019 |
| Esteem Pro UK Limited | Wholesale & retail | 2019 |
| Estradda 89 Ltd | Transport & logistics | 2021 |