| Eminence Services Ltd | Administrative & support | 2025 |
| Eminent UK Catering Ltd | Hospitality | 2023 |
| Emir Estate Limited | Real estate | 2015 |
| Emirage Ltd | Financial services | 2024 |
| EMJ Content Ltd | Information & communication | 2023 |
| Emma Bridgeman Styling & Art Direction Ltd | Administrative & support | 2003 |
| Emma Housham Limited | Other services | 2012 |
| Emma Moate Camera Operator Ltd | Information & communication | 2019 |
| Emma Tuby Properties Ltd | Real estate | 2023 |
| Emmaway Ltd | Real estate | 2014 |
| Emmerson-Ward TM Ltd | Public administration | 2025 |
| Emmest Cleaning Services Limited | Administrative & support | 2026 |
| Emms & Sons Limited | Wholesale & retail | 1961 |
| Emms Dental Practice Limited | Health & social care | 2011 |
| Emmycares Health Solutions Ltd | Health & social care | 2023 |
| Emotion Explorers Ltd | Information & communication | 2024 |
| Emotional Development Mentoring Ltd | Extraterritorial | 2020 |
| Empire and Spire Limited | Wholesale & retail | 2023 |
| Empire Asset Management Limited | Financial services | 2008 |
| Empire Commerce House Limited | Financial services | 2019 |
| Empire Cuts Barber Shop Ltd | Other services | 2021 |
| Empire Engineering Consultants Ltd | Professional & scientific | 2016 |
| Empire Home and Garden Ltd | Wholesale & retail | 2025 |
| Empire Interior Solutions Ltd | Construction | 2017 |
| Empire Modular Limited | Real estate | 2017 |
| Empire Pavilion Development Limited | Financial services | 2019 |
| Empire Plumbing and Heating Services Limited | Construction | 2025 |
| Empire Property Finance 1 Limited | Professional & scientific | 2019 |
| Empire Railway Limited | Financial services | 2019 |
| Empire Real Estate & Development Ltd | Construction | 2021 |
| Empire Roofing & Gutters Ltd | Construction | 2026 |
| Empire Waterdale Limited | Financial services | 2019 |
| Empire Wharncliffe Limited | Financial services | 2019 |
| Employment Factors International Ltd | Administrative & support | 2021 |
| Employment Support Ltd | Administrative & support | 2018 |
| Empowering Minds Academy Trust | Education | 2017 |
| Empowr Group Ltd | Professional & scientific | 2025 |
| Empress Sales Ltd | Wholesale & retail | 2024 |
| Emro 12 Limited | Financial services | 2020 |
| EMS Hydraulic Services Limited | Manufacturing | 2014 |
| Emsat Communications Limited | Wholesale & retail | 2002 |
| EMY Connect Ltd | Transport & logistics | 2025 |
| EN Video Production Ltd | Information & communication | 2023 |
| Enable Energy Ltd | Professional & scientific | 2022 |
| Enabling Solutions Limited | Financial services | 2006 |
| Encaustic Art UK Ltd | Wholesale & retail | 2022 |
| Enchanted Escapes Travel Ltd | Administrative & support | 2023 |
| Enchanted Gardens Day Nursery Ltd | Health & social care | 2026 |
| Encircle Marketing Limited | Professional & scientific | 2007 |
| Encom Telecommunications Limited | Information & communication | 2004 |
| END 2 END Recruitment Limited | Administrative & support | 2020 |
| Endeavour Lifestyle Limited | Education | 2021 |
| Endless Delivery Ltd | Transport & logistics | 2023 |
| Endurance Trans Limited | Manufacturing | 2023 |
| ENE Logistics Limited | Transport & logistics | 2018 |
| Eneci Ltd | Transport & logistics | 2025 |
| Eneg Ltd | Wholesale & retail | 2019 |
| Energise Access Solutions Limited | Construction | 2024 |
| Energise Eco Solutions Limited | Professional & scientific | 2025 |
| Energise Electrical & Solar Ltd | Construction | 2024 |
| Energise Energy Solutions Ltd | Professional & scientific | 2018 |
| Energize Installations Ltd | Other services | 2019 |
| Energizing Tomorrow's Tech Ltd | Construction | 2025 |
| Energy Dance and Performing Arts Ltd | Arts & recreation | 2024 |
| Energyaware Ltd | Professional & scientific | 2017 |
| Energymiser Ltd | Manufacturing | 2021 |
| Enescu Solutions Ltd | Administrative & support | 2025 |
| Enfuse Plumbing and Building Services Ltd | Construction | 2020 |
| Engage British Stem Ltd | Education | 2019 |
| Engineered Projects Solutions Ltd | Other services | 2013 |
| Engineering & Technology Limited | Extraterritorial | 2026 |
| Engineering Planning Ltd | Construction | 2015 |
| Engineering Pressure and Vacuum Services Ltd | Professional & scientific | 2025 |
| Engineering Site Solutions Ltd | Administrative & support | 2014 |
| Engineering Support Group Limited | Professional & scientific | 1995 |
| English International Online Ltd | Education | 2020 |
| Enhance Production Limited | Information & communication | 2023 |
| Enhance Property Experts Limited | Construction | 2013 |
| Enhancement Training Limited | Administrative & support | 2009 |
| Enhancements of Elegance Ltd | Other services | 2021 |
| ENI Europe Ltd | Professional & scientific | 2007 |
| Enies Solutions Ltd | Information & communication | 2023 |
| Enigma Rooms Leisure (Doncaster) Ltd | Arts & recreation | 2026 |
| Enigma Rooms Leisure (Hull) Ltd | Arts & recreation | 2026 |
| Enigma Rooms Leisure (Pontefract) Ltd | Arts & recreation | 2026 |
| Enigma Rooms Leisure Ltd | Arts & recreation | 2026 |
| Enigma Rooms Ltd | Arts & recreation | 2018 |
| Enik Ltd | Hospitality | 2021 |
| Enjoy Investments Limited | Real estate | 2022 |
| Ennerdale Estates Limited | Real estate | 2020 |
| Ennjay Solutions Ltd | Administrative & support | 2014 |
| Enoptron Limited | Professional & scientific | 2006 |
| Enpower Consulting Ltd. | Financial services | 2018 |
| Ensynergy International (UK) Limited | Information & communication | 1999 |
| Ensynergy Technology Limited | Information & communication | 1998 |
| Enterprise Accountancy Services Limited | Professional & scientific | 2002 |
| Enterprise Locomotives Ltd | Manufacturing | 2007 |
| Entervex Limited | Information & communication | 2025 |
| Entirety Couture Limited | Wholesale & retail | 2026 |
| Envelop Business Solutions Ltd | Professional & scientific | 2022 |