| SES (Specialist Engineering) Group Limited | Transport & logistics | 2008 |
| SES Rail Limited | Transport & logistics | 2012 |
| Setway AI Ltd | Information & communication | 2025 |
| Seven Hills Bakery Limited | Hospitality | 2011 |
| Seven Plus One Ltd | Hospitality | 2025 |
| Seven Sins Customs Limited | Manufacturing | 2017 |
| Seven Star to LET Ltd | Real estate | 2026 |
| Seven Studios (Doncaster) Limited | Information & communication | 2023 |
| Seven Yards Properties Limited | Real estate | 2022 |
| Seventeen Power Limited | Wholesale & retail | 2023 |
| Severals Farm Limited | Agriculture & fishing | 1978 |
| Severals Ventures Limited | Hospitality | 2013 |
| Severn Waste Services Limited | Energy | 1998 |
| SEW Mindful Crafts CIC | Education | 2022 |
| SFA Transport Ltd | Transport & logistics | 2025 |
| Sfau Foods Ltd | Hospitality | 2024 |
| Sfbibawy Limited | Health & social care | 2022 |
| SG Networks Ltd | Wholesale & retail | 2021 |
| Sg.Cableties Ltd | Wholesale & retail | 2020 |
| SGG Trans Ltd | Transport & logistics | 2018 |
| SGH Civils and Surfacing Limited | Construction | 1996 |
| SGH Construction (Holdings) Ltd | Financial services | 2025 |
| SGH Construction Group Limited | Financial services | 2021 |
| SGH Plant Hire Limited | Administrative & support | 1997 |
| SGH Surfacing Limited | Construction | 2020 |
| Sgip Ltd | Professional & scientific | 2022 |
| SGL Bearings Ltd | Wholesale & retail | 2022 |
| SGN Consultancy Ltd | Professional & scientific | 2024 |
| Sgtakeaway Limited | Hospitality | 2023 |
| SGX Solutions Limited | Transport & logistics | 2022 |
| SH & J Ltd | Administrative & support | 2013 |
| SH and SON Ltd | Real estate | 2024 |
| SH TM Ltd | Information & communication | 2024 |
| SH! Dance Limited | Arts & recreation | 2018 |
| SHA Super Markett Limited | Wholesale & retail | 2025 |
| Shabbs TM Ltd | Public administration | 2024 |
| Shabby Doll Records Limited | Information & communication | 2013 |
| Shadenburg Consulting Ltd | Professional & scientific | 2019 |
| Shades of Doncaster Limited | Manufacturing | 1989 |
| Shadow Utilities Ltd | Construction | 2013 |
| Shaftesbury Avenue Burton Joyce Management Company Limited | Households | 2006 |
| Shah Trader Enterprises Ltd | Hospitality | 2025 |
| Shah's Unify International Ltd | Wholesale & retail | 2023 |
| Shahfwdenterprises Ltd | Wholesale & retail | 2025 |
| Shaka Traders Limited | Wholesale & retail | 2025 |
| Shakespeare Surgery Ltd. | Health & social care | 2003 |
| Sham Builders Ltd | Construction | 2023 |
| Shama Balti House Ltd | Hospitality | 2026 |
| Shane Butler Safety Solutions Ltd | Construction | 2021 |
| Shane Hotterwell Ltd | Construction | 2022 |
| Shang HAI Chinese Takeaway Ltd | Hospitality | 2026 |
| Shanghe Tech Limited | Wholesale & retail | 2026 |
| Shannon Davis Limited | Education | 2022 |
| Shansai Limited | Wholesale & retail | 2020 |
| Shansai Properties Limited | Real estate | 2023 |
| Shape House (Doncaster) Ltd | Education | 2025 |
| Shape House Arts Community Interest Company | Arts & recreation | 2025 |
| Shape Management Limited | Real estate | 2008 |
| Shapeshyft Ltd | Administrative & support | 2021 |
| Shared Spaces Collective Ltd | Households | 2025 |
| Sharigon Limited | Wholesale & retail | 1999 |
| Sharing Hands Limited | Health & social care | 2016 |
| Sharon's Childcare Services Limited | Health & social care | 2023 |
| Sharp Clouds IT and Multi Services Ltd | Information & communication | 2019 |
| Sharp Strandz Ltd | Other services | 2024 |
| Sharrow Vale Hardware Ltd | Wholesale & retail | 2013 |
| Sharvillefabrication Ltd | Manufacturing | 2025 |
| Shattered Aces Limited | Wholesale & retail | 2026 |
| Shaun Flannery Photography Limited | Professional & scientific | 2001 |
| Shaun Goulding Limited | Transport & logistics | 2021 |
| Shaun Mcarthur Properties Doncaster Limited | Real estate | 2017 |
| Shaun Walker Limited | Administrative & support | 2022 |
| Shaun Ward Properties Ltd | Real estate | 2023 |
| Shaw Bros. Limited | Construction | 2001 |
| Shaw Brothers Estates Ltd | Real estate | 2024 |
| Shaw Eco Solutions Limited | Construction | 2021 |
| Shaw Group Limited | Administrative & support | 2020 |
| Shaw Key Properties Limited | Construction | 2025 |
| Shaw Lane Bakery Limited | Real estate | 2023 |
| Shaw Secure Ltd | Construction | 2016 |
| Shawbyte Labs Limited | Information & communication | 2026 |
| Shawcross Care Ltd | Health & social care | 2023 |
| Shaws Management Ltd | Professional & scientific | 2024 |
| Shaz (Sheff) Ltd | Hospitality | 2019 |
| Sheabynature Ltd | Manufacturing | 2017 |
| Sheardown Logistics Limited | Transport & logistics | 2017 |
| Shearman Developments Ltd | Construction | 2024 |
| Shebas PUB Ltd | Extraterritorial | 2024 |
| Sheds HUT Limited | Wholesale & retail | 2009 |
| Shedseal Ltd | Wholesale & retail | 2023 |
| Sheepish Productions Theatre Company Limited | Arts & recreation | 2022 |
| Sheffield & District Society of Chartered Accountants | Professional & scientific | 1882 |
| Sheffield Dyslexia Centre Limited | Education | 2016 |
| Sheffield Housing Company Limited | Construction | 2011 |
| Sheffield Mount Carmel UK Ltd | Real estate | 2021 |
| Shehjaar Ltd | Wholesale & retail | 2023 |
| Shehzade Ltd | Professional & scientific | 2026 |
| Sheikh Construction Ltd | Construction | 2014 |
| Shekinah 'S Real Estate Ltd | Hospitality | 2023 |
| Shelbourne Engineering Limited | Manufacturing | 2015 |