| Keycraft Garden Buildings Limited | Manufacturing | 2001 |
| Kieran Mullin Limited | Other services | 1999 |
| KIM Holmes Show Business Entertainment Agency Limited | Arts & recreation | 2005 |
| Kinchbus Limited | Transport & logistics | 1987 |
| Kingfield Group Holdings Limited | Other services | 2025 |
| Kingsmill Industries (UK) Limited | Manufacturing | 1998 |
| Kingston Contracts Limited | Professional & scientific | 2008 |
| Kirkwall Securities Limited | Construction | 1999 |
| Kirrage Williams Limited | Financial services | 1988 |
| Kleanline Limited | Administrative & support | 1993 |
| KMS Fabrications Limited | Manufacturing | 2005 |
| Koyesir Ltd | Wholesale & retail | 2022 |
| Kwil Business Services Limited | Professional & scientific | 2005 |
| L & K Clarke Psychology Services Limited | Health & social care | 2003 |
| L & S Accountancy Services Ltd | Professional & scientific | 2007 |
| L.A. Engineering (Belper) Limited | Professional & scientific | 1997 |
| L.Middleton(Ripley)Limited | Wholesale & retail | 1963 |
| Langley Priory Farm Limited | Agriculture & fishing | 1996 |
| Laser Systems (UK) Limited | Manufacturing | 2005 |
| Last BET Limited | Information & communication | 2007 |
| Last Minute Check Limited | Information & communication | 2007 |
| Layden Court Care Home Ltd | Health & social care | 2021 |
| LEA Rhododendron Gardens Limited | Agriculture & fishing | 1960 |
| Leadership Engineering and Process Services Ltd | Professional & scientific | 2015 |
| Lean TPS Limited | Professional & scientific | 2006 |
| Leasetoday Limited | Real estate | 1992 |
| LEE Brown Joinery Limited | Construction | 2005 |
| Legal Support Services (Northern) Ltd | Professional & scientific | 2007 |
| Lemon and Black Group Limited | Wholesale & retail | 2022 |
| Leon Haslam Limited | Arts & recreation | 2005 |
| Lewis & Sons Precious Metal Recovery Ltd | Transport & logistics | 2016 |
| Lewis Bespoke Joinery Limited | Construction | 2023 |
| Liaison Life Insurance and Investment Services of Nottingham Limited | Financial services | 1968 |
| Lift Safe Crane Services Ltd | Manufacturing | 2018 |
| Lifting Systems Ltd. | Manufacturing | 1994 |
| Light Hills Property Ltd | Real estate | 2024 |
| Lillex Cosmetics Ltd | Manufacturing | 2025 |
| Lillex Ltd | Wholesale & retail | 2025 |
| Lillex Recruitment Ltd | Administrative & support | 2026 |
| Linda Cotterill Limited | Other services | 2006 |
| Lindway Scaffolding Limited | Construction | 1983 |
| Lingley Green Management Company Limited | Households | 2018 |
| Linx Business Communications Limited | Information & communication | 2003 |
| Lister Equipment Limited | Manufacturing | 1959 |
| Little Buddies Limited | Transport & logistics | 2005 |
| Little Leprechauns Day Nurseries Limited | Health & social care | 2005 |
| Littleover (Blair House) Properties Limited | Real estate | 1975 |
| Living Designs Limited | Professional & scientific | 2005 |
| LLW Rentals Ltd | Other services | 2022 |
| Lowe Developments Limited | Construction | 2003 |
| Lowe Leisure Limited | Hospitality | 2005 |
| LPE Groundworks Ltd | Construction | 2024 |
| LS Plant & Haulage Limited | Extraterritorial | 2018 |
| Lunnun Trading and Sales Ltd | Agriculture & fishing | 2002 |
| M & M Holdings (Derby) Limited | Professional & scientific | 2006 |
| M & M Landscapes (Measham) Limited | Other services | 2003 |
| M Bennett Limited | Construction | 2003 |
| M D Scaffolding Services Ltd | Construction | 2006 |
| M F Wardman Limited | Transport & logistics | 2003 |
| M L Clayton Plant & Agriculture Ltd | Construction | 2003 |
| M-Track UK Limited | Wholesale & retail | 1997 |
| M.C. Northern Limited | Water & waste | 2000 |
| M.I.P. Developments Limited | Construction | 1995 |
| Mabe Allen LLP | | 2004 |
| Macgregor Building & Roofing Limited | Construction | 2023 |
| Machine Building Systems Limited | Manufacturing | 1989 |
| Maine Street Ltd | Professional & scientific | 2008 |
| Majic Rental Services Limited | Real estate | 2004 |
| Makin Builders Limited | Construction | 2004 |
| Making Plans UK Limited | Professional & scientific | 2007 |
| Manchester Xtreme Ltd | Wholesale & retail | 2007 |
| Mandene Limited | Transport & logistics | 1973 |
| Manor Garage Limited | Wholesale & retail | 2003 |
| Marblex Limited | | 1969 |
| Marcham Meadows Management Company Limited | Households | 2025 |
| Mark Bayley Limited | Wholesale & retail | 2002 |
| Mark Beresford Transport Limited | Transport & logistics | 2002 |
| Mark Heywood (Electrical Contractors) Limited | Construction | 2004 |
| Marshall and Till (Belper) Ltd. | Professional & scientific | 2007 |
| Marshall Developments (Derby) Limited | Professional & scientific | 2006 |
| Marshall Edit Ltd | Wholesale & retail | 2026 |
| Marshall Rowell & Company Limited | Wholesale & retail | 1954 |
| Martin Ogden (Butchers) Limited | Wholesale & retail | 2005 |
| Mason Electronics Limited | Information & communication | 1999 |
| Mason's Coaches Limited | Transport & logistics | 2003 |
| Masonsoft Technology Limited | Information & communication | 2007 |
| Matglass Limited | Construction | 1993 |
| Matlock Antiques Limited | Arts & recreation | 2006 |
| Matlock Bath Balti Limited | Hospitality | 2003 |
| Matlock Properties,Limited(The) | Real estate | 1943 |
| Matlock Sports Club Limited | Arts & recreation | 1921 |
| Matlow Brothers Limited | Extraterritorial | 1934 |
| Matthew Lane Security Ltd | Administrative & support | 2015 |
| MAX Healthcare Limited | Wholesale & retail | 2005 |
| MAX Remedies Limited | Wholesale & retail | 2007 |
| MC2 Limited | Professional & scientific | 1998 |
| Mcmurtry & Harding | | 2006 |
| MCR Joinery & Interiors Limited | Manufacturing | 2020 |
| MD & BW Buxton Limited | Construction | 2003 |
| MDS Construction Limited | Construction | 2003 |