| High Peak Signs Ltd. | Manufacturing | 2004 |
| High Street Bodyworks Ltd | Wholesale & retail | 2005 |
| Highedge Solutions Limited | Professional & scientific | 2006 |
| Hill & Webster (Handling) Limited | Manufacturing | 1979 |
| Hill & Webster (Services) Limited | Professional & scientific | 1985 |
| Hilton Property Group Ltd | Construction | 2023 |
| Hoben International Limited | Manufacturing | 1963 |
| Holyrood Housing Limited | Real estate | 2003 |
| Home-Start Erewash | Professional & scientific | 2004 |
| Hoopers (Derby) Limited | Other services | 1997 |
| Hopping Farm Limited | Hospitality | 2000 |
| Hornsby Holdings Limited | Professional & scientific | 1994 |
| Hornsby Plant Hire Limited | Administrative & support | 1983 |
| Hotspa Limited | Financial services | 2024 |
| House of Ingle Asset Management Limited | Real estate | 1999 |
| Howard Brothers Limited | Arts & recreation | 2004 |
| Howard Carter Scientific Industries (U.K.) Limited | | 1981 |
| HPC Drives Limited | Professional & scientific | 2000 |
| HRA Properties Ltd | Other services | 2004 |
| Huey Games Ltd | Information & communication | 2013 |
| Hulland Ward Garage Limited | Wholesale & retail | 1971 |
| Hydrophilous Limited | Manufacturing | 2008 |
| I Barker Ltd | Professional & scientific | 2002 |
| I/N Consultants Ltd | Information & communication | 2001 |
| I3S Limited | Extraterritorial | 2003 |
| IB Joinery Services Limited | Professional & scientific | 2003 |
| Ideapie Limited | Professional & scientific | 2006 |
| Ilkeston Rugby Union Football Club Limited | Arts & recreation | 2006 |
| Ilkeston Town FC Academy Limited | Education | 2019 |
| Imagine Event Design Limited | Other services | 2014 |
| Income Advisory Services Limited | Financial services | 1994 |
| Independent Will Makers Limited | Professional & scientific | 2007 |
| Indigoblue Consultants Limited | Professional & scientific | 2007 |
| Indigoextra Limited | Information & communication | 2006 |
| Individual Savings Accounts Limited | Financial services | 1997 |
| Industrial Electrical Projects Limited | Professional & scientific | 2006 |
| Infrasig Limited | Manufacturing | 2012 |
| Ingleby Design & Management Limited | Professional & scientific | 2007 |
| Inson Properties Limited | Construction | 2001 |
| Integrated Project Services Limited | Construction | 1999 |
| Interior Design Associates Ltd | Other services | 2000 |
| Introspective Ltd | Other services | 2017 |
| Inverter Drive Systems Limited | Construction | 1995 |
| Isoma Limited | Manufacturing | 1997 |
| IVY Developments (Derby) Limited | Real estate | 2005 |
| J B Services (Derby) Limited | Water & waste | 2000 |
| J C Balmforth Limited | Manufacturing | 1996 |
| J P Bookkeeping & Payroll Services Limited | Professional & scientific | 2018 |
| J P Webster Joinery Limited | Construction | 2008 |
| J S Atherton & SON Limited | Hospitality | 2005 |
| J S D (Derby) Limited | Wholesale & retail | 2006 |
| J T Mcneil Holdings Ltd | Financial services | 2024 |
| J.B.Joyce & Company Limited | Manufacturing | 1912 |
| J.C. Walwyn & Sons Limited | Manufacturing | 1985 |
| J.R.N. Fireplace Supplies Limited | Wholesale & retail | 2000 |
| J.T. Somerville (Decorators) Ltd | Construction | 2003 |
| J.T.D. Electronics Limited | Manufacturing | 1999 |
| Jaam Properties Limited | Real estate | 2004 |
| James Marcus Kitchens Limited | | 1980 |
| Janjua Vision Limited | Wholesale & retail | 2003 |
| JDS Distributors Limited | Extraterritorial | 1959 |
| JIH Electrical Ltd | Construction | 2014 |
| JOB Earn Ltd | Administrative & support | 2024 |
| John Ault Automation Limited | Professional & scientific | 1998 |
| John Davies Electrical Contractor Limited | Construction | 1999 |
| John Day (Decorators) Limited | Construction | 1998 |
| John Mills Curtains Limited | Wholesale & retail | 1980 |
| John Pepper (Optical) Limited | Health & social care | 1968 |
| John R. Crossland Construction Limited | Construction | 1984 |
| Johnsson Limited | Wholesale & retail | 2025 |
| Johnstone Consulting LLP | | 2005 |
| Jojo Harris Limited | Wholesale & retail | 2024 |
| JON Moore Property Improvements Limited | Construction | 2003 |
| Josh Morley Golf Coaching Ltd | Arts & recreation | 2025 |
| JPC Services (Glossop) Limited | Other services | 2005 |
| JPF CAD Limited | Construction | 2007 |
| JPS Heating Limited | Construction | 2000 |
| Jrwg Services Ltd. | Other services | 2025 |
| JTP Heating Limited | Construction | 2007 |
| Judy Leden MBE Ltd | Transport & logistics | 2003 |
| Juice Electrical Supplies Limited | Wholesale & retail | 2007 |
| Justice and Hazelwood Limited | Professional & scientific | 2005 |
| Justsloggi Limited | Wholesale & retail | 2005 |
| K & B Rouse Construction Limited | Construction | 1997 |
| K & B Rouse Holdings Limited | Administrative & support | 2007 |
| K. L. Macadam Limited | Construction | 2005 |
| K. Wolverson Electrical Services Limited | Construction | 2006 |
| K.H. Window Cleaning Limited | Administrative & support | 2003 |
| K.N.Ellis Consultancy Ltd | Information & communication | 2005 |
| K.R.M. Building Supplies Limited | Wholesale & retail | 1989 |
| K2AUDIO Ltd | Manufacturing | 2018 |
| Katapult Limited | Professional & scientific | 2001 |
| Kate Tomlinson Ltd | Health & social care | 2003 |
| Katie Wells Homecare Limited | Construction | 2004 |
| KBB Solutions Limited | Professional & scientific | 2006 |
| KCM Inflatables Ltd | Arts & recreation | 2019 |
| Kedleston Interiors Limited | Wholesale & retail | 2004 |
| Keljay Shotblasting & Refurbishments Ltd | Manufacturing | 2014 |
| Key Accounting Limited | Professional & scientific | 2006 |
| Keyasset Residents Management Limited | Real estate | 1986 |