| Banks Skip Hire Limited | Water & waste | 2003 |
| Barkeng MAD Limited | Manufacturing | 2007 |
| Barker & Co Accountancy Services Ltd | Extraterritorial | 2007 |
| Barn Farm Campsite Limited | Arts & recreation | 2005 |
| Barn Farm Store Limited | Professional & scientific | 2005 |
| Barton Buses Limited | Transport & logistics | 1989 |
| Bateman Consulting Services Ltd | Information & communication | 2002 |
| Baylinks Limited | Extraterritorial | 1989 |
| BB Ashbourne Limited | Professional & scientific | 2001 |
| BB Vending Services Limited | Administrative & support | 2008 |
| BDS Architecture (Derby) Ltd | Professional & scientific | 2004 |
| Beechwood (Derby) Limited | Wholesale & retail | 1986 |
| Beespace Developments Ltd | Real estate | 2024 |
| Beighton Construction Limited | Construction | 1971 |
| Belfield Engineering Limited | Manufacturing | 2005 |
| Belle Engineering (Sheen) Limited | Manufacturing | 1961 |
| Belle Holdings Limited | Manufacturing | 1997 |
| Belper Scaffold Services Ltd | Construction | 2007 |
| Bembeno LLP | | 2024 |
| BEN Cope Limited | Transport & logistics | 1959 |
| Benring Limited | Manufacturing | 1997 |
| Bentley Bridge Ltd | Real estate | 2023 |
| Berne Welbeck Limited | Manufacturing | 1990 |
| Berrisford Sweeper Hire Ltd | Water & waste | 2005 |
| Bestwood Woodcare Limited | Wholesale & retail | 2006 |
| Betts Bins & Window Cleaning Services Ltd | Administrative & support | 2021 |
| Bexon Heaps Associates Ltd | Professional & scientific | 2017 |
| Bharasim Limited | Transport & logistics | 2017 |
| BI Design Architecture Limited | Professional & scientific | 2003 |
| Big on Bikes Limited | Manufacturing | 2003 |
| Billy Holmesdale Premier Ltd | Wholesale & retail | 2022 |
| Binary Consultancy Limited | Other services | 1998 |
| Bink Systems Limited | Information & communication | 1990 |
| Bionical Solutions Group Limited | Financial services | 2018 |
| Birchwood Homecaring Services Limited | Health & social care | 2000 |
| Birlea Furniture Limited | Wholesale & retail | 2001 |
| Bison B Composites Ltd | Construction | 2024 |
| Black Art Limited | Real estate | 1991 |
| Blackdew Limited | Real estate | 1987 |
| Bladon Finance Limited | Administrative & support | 2021 |
| Blakes the Art of Hair Limited | Other services | 2002 |
| Blue Sycamore Limited | Professional & scientific | 2004 |
| Blue Wizard (Chesterfield) Limited | Hospitality | 2006 |
| Blueearth Environmental Limited | Professional & scientific | 2020 |
| Blueearth Marine Limited | Administrative & support | 2020 |
| Blueearth Recycling Limited | Water & waste | 2020 |
| Blueflame Heatplan Limited | Construction | 2001 |
| Bluejohn Marketing Ltd | Professional & scientific | 2003 |
| Bombardier Transportation (Gautrain) UK Limited | Manufacturing | 1995 |
| BON Appetit (Derby) Limited | Real estate | 2004 |
| Bondteam Limited | | 1982 |
| Bonneville Coventry Limited | Professional & scientific | 1988 |
| Booze City Limited | Wholesale & retail | 2007 |
| Bottle Green Training Limited | Education | 1999 |
| Bowden Head Farmhouse Limited | Hospitality | 1996 |
| Bowmer Bond (Holdings) Limited | Professional & scientific | 2007 |
| Bradbury Property Co. Limited | Real estate | 1947 |
| Bradwell Properties Limited | Extraterritorial | 2004 |
| Brailsfordg Limited | Wholesale & retail | 2025 |
| Breaston Electrical Services Limited | Construction | 2006 |
| Brereton Services Limited | Financial services | 2007 |
| Bretby Brick and Stoneware Company Limited | Real estate | 1909 |
| Bridge Garage (Swadlincote) Limited | Wholesale & retail | 2005 |
| Brinard Joinery Limited | Construction | 1986 |
| Broadholme Ltd | Professional & scientific | 2004 |
| Broadway Nursing Limited | | 1989 |
| Brookfield Farming Ltd | Agriculture & fishing | 2008 |
| Brookfields P.N.H. Limited | Health & social care | 1992 |
| Browns Builders Merchants Limited | Wholesale & retail | 1990 |
| Brunel Healthcare Manufacturing Limited | Manufacturing | 2002 |
| Bubu Alin Ltd | Transport & logistics | 2019 |
| Bull Construction UK Ltd | Construction | 2007 |
| Burns & Cooper Limited | Other services | 2003 |
| Bursa Holdings Limited | Extraterritorial | 2018 |
| Business Electricity UK Limited | Energy | 2007 |
| Business Innovation Deployment Ltd | Professional & scientific | 2017 |
| Business Services Agency Limited | Professional & scientific | 1996 |
| Button Management Training Limited | Professional & scientific | 2000 |
| Buxton Arts Festival Limited | Arts & recreation | 1978 |
| Buxton Builders Limited | | 1975 |
| Buxton Building Supplies Limited | Manufacturing | 1998 |
| Buxton Dairy Supplies Limited | Real estate | 2001 |
| Buxton Football Club Limited | Arts & recreation | 1993 |
| Buxton Press Limited | Manufacturing | 1960 |
| C Haynes Ltd | Health & social care | 2006 |
| C J Wilson Stocktakers Limited | Professional & scientific | 2004 |
| C R D Birtles Limited | Construction | 2003 |
| C Tomlinson Electrical Services Limited | Construction | 2007 |
| C.R. Clowes and SON Limited | Wholesale & retail | 1990 |
| Cadman Electrical Systems Ltd | Manufacturing | 2014 |
| Calamata Three Limited | Administrative & support | 2005 |
| Calderbank Contractors Limited | Construction | 1995 |
| Can-Du Architecture Ltd | Professional & scientific | 2023 |
| Capital Limousines Midlands Limited | Transport & logistics | 2007 |
| Capital Refractories Holdings Limited | Financial services | 2025 |
| Capital Refractories Limited | Manufacturing | 1973 |
| Capricorn Hair Studio Limited | Other services | 2006 |
| Car Care Centre (Padfield) Limited | Wholesale & retail | 2007 |
| Caralec Limited | Real estate | 1983 |
| Carcoon Storage Systems (International) Limited | Manufacturing | 1994 |