| EM Property Services (East Midlands) Ltd | Administrative & support | 2026 |
| EMA & DUA Ltd | Manufacturing | 2024 |
| EMA Training Limited | Education | 2005 |
| Eman Derby Ltd | Wholesale & retail | 2024 |
| Eman Retail Ltd | Wholesale & retail | 2025 |
| Emata Limited | Transport & logistics | 2020 |
| Embark Education Services Limited | Administrative & support | 2024 |
| Embark Multi Academy Trust | Education | 2018 |
| Embedded Rail Technology Limited | Professional & scientific | 2000 |
| Emberbox Limited | Information & communication | 2025 |
| Embers Fitting Solutions Limited | Wholesale & retail | 2010 |
| Embers Heating Studio Limited | Wholesale & retail | 2015 |
| Embg Limited | Arts & recreation | 2003 |
| Emblem Imports Limited | Professional & scientific | 1993 |
| Emblem Print Products Limited | Administrative & support | 1989 |
| Embracent Limited | Information & communication | 2015 |
| Embs Recruitment Ltd | Professional & scientific | 2018 |
| Embs Training Limited | Information & communication | 2026 |
| EMC Building Ltd | Construction | 2022 |
| Emccan C.I.C. | Other services | 2012 |
| EMD Care Ltd | Health & social care | 2023 |
| EMD Holdings Limited | Professional & scientific | 2020 |
| EMD Property Limited | Real estate | 2023 |
| EME Tools Ltd | Manufacturing | 2016 |
| Emerald (Eco) Ltd | Agriculture & fishing | 2026 |
| Emerald Business Bureau Ltd | Administrative & support | 2012 |
| Emerald Fabrications Limited | Manufacturing | 2020 |
| Emerald Home Improvements (Leics) Limited | Construction | 2005 |
| Emerald Home Improvements Ltd | Construction | 2008 |
| Emerald UK Investments Ltd | Real estate | 2021 |
| Emerald Windows Limited | Real estate | 2005 |
| Emergency Business Support Limited | Financial services | 2022 |
| Emergency Medical Associates Ltd | Education | 2015 |
| Emery Prop Co Ltd | Real estate | 2026 |
| Emga Limited | Professional & scientific | 2008 |
| Emijo Limited | Wholesale & retail | 2026 |
| Emily Chappell Ltd | Information & communication | 2024 |
| Emily's Steakhouse Ltd | Hospitality | 2025 |
| Emlis Healthcare Limited | Health & social care | 2025 |
| Emma Bowmar Limited | Arts & recreation | 2009 |
| Emmadanso Transport and Logistics Company Limited | Transport & logistics | 2023 |
| Emmanuel School (Derby) Limited | Education | 2019 |
| Emmas Emporium CIC | Wholesale & retail | 2023 |
| Emma’S Emporium of Extraordinary Things Limited | Wholesale & retail | 2019 |
| Emmerson Dutton Limited | Financial services | 2014 |
| Emmric Holdings Limited | Financial services | 2023 |
| Emmric Limited | Wholesale & retail | 2023 |
| Emotion Entertainment Expedition Ltd | Arts & recreation | 2024 |
| Empal Limited | Professional & scientific | 2018 |
| Empathix Ltd | Information & communication | 2024 |
| Empathy 4 ALL Ltd | Administrative & support | 2023 |
| Emperus Limited | Professional & scientific | 2020 |
| Empire & Co Residential Ltd | Real estate | 2021 |
| Empire Amusements Limited | Arts & recreation | 2023 |
| Empire Capital (Derbyshire) Limited | Construction | 2011 |
| Empire GEN Construction Limited | Construction | 2021 |
| Empire Group Property Holdings Ltd | Real estate | 2024 |
| Empire Homes Group Ltd | Construction | 2021 |
| Empire Personnel Limited | Information & communication | 2008 |
| Empire Property Associates Ltd | Real estate | 2020 |
| Empire Property Maintenance Limited | Construction | 2006 |
| Empire Readymix Ltd | Manufacturing | 2020 |
| Empire Tandoori Spondon Limited | Hospitality | 2023 |
| Empower Educational Psychology Ltd | Education | 2021 |
| Empower Tech Support Services Ltd | Information & communication | 2024 |
| Empowered Aesthetics Derby Limited | Other services | 2019 |
| Empowered Graceful Care Ltd | Health & social care | 2018 |
| Empowered Training Solutions & Services Ltd | Education | 2025 |
| Empowered Women Collective Ltd | Education | 2026 |
| Empowerher Network CIC | Health & social care | 2025 |
| Empowermebyjodiebirkinshaw Ltd | Arts & recreation | 2026 |
| Empress Property Co Ltd | Real estate | 2020 |
| Empress WAX Ltd | Wholesale & retail | 2021 |
| Emptor Plus Ltd | Professional & scientific | 2011 |
| Empyrean Care Group Ltd | Health & social care | 2016 |
| Emris Retreats Ltd | Real estate | 2025 |
| Emrys Oak Property Company Ltd | Real estate | 2023 |
| EMS Property Holdings Limited | Real estate | 2020 |
| Emsec Group Holdings Ltd | Financial services | 2022 |
| Emtd Limited | Professional & scientific | 2014 |
| Emtopia HR and Recruitment Ltd | Administrative & support | 2024 |
| EMU Property Limited | Real estate | 2022 |
| Emunah Limited | Real estate | 2017 |
| EMY Care Limited | Health & social care | 2015 |
| Emylin Limited | Transport & logistics | 2010 |
| Enable Others Derby Ltd | Education | 2024 |
| Enable Support Limited | Public administration | 2019 |
| Enabling Independence OT Services Ltd | Other services | 2017 |
| Enamdo Global Ltd | Wholesale & retail | 2026 |
| ENC Thrive Stays Ltd | Hospitality | 2025 |
| Encalmo Ltd. | Information & communication | 2017 |
| Encapusula Networks Ltd | Information & communication | 2025 |
| Encompass Engineering Solutions Limited | Professional & scientific | 2007 |
| Encompass Foods Limited | Hospitality | 2019 |
| Encore Aero Limited | Manufacturing | 2016 |
| Encore Wholesale Ltd | Wholesale & retail | 2018 |
| Endee Holdings Limited | Manufacturing | 2017 |
| Endofuze Ltd | Health & social care | 2025 |
| Energee Derby Limited | Other services | 2021 |
| Energielink Limited | Manufacturing | 2023 |