| Umayya Investments Ltd | Hospitality | 2019 |
| Umbrella Derby & Derbyshire | Health & social care | 2012 |
| Umbrella Force Group INT Ltd | Construction | 2016 |
| Umbrella Force Group Ltd | Construction | 2014 |
| Umbrella Force Ltd | Professional & scientific | 2017 |
| Umewe Ltd | Health & social care | 2026 |
| UMI Estates Limited | Professional & scientific | 2026 |
| Umora Ltd | Wholesale & retail | 2021 |
| Umzuri Ltd | Information & communication | 2022 |
| UN Investments Limited | Real estate | 2025 |
| Uncle DEE Autos Ltd | Wholesale & retail | 2025 |
| Under Pressure Exterior Cleaning & Maintenance Ltd | Administrative & support | 2025 |
| Underground Drainage Supplies Limited | Wholesale & retail | 2010 |
| Underhill Property Investments Limited | Real estate | 2017 |
| Underwood Vinecombe LLP | | 2008 |
| Unheard Voice Consultancy Ltd | Administrative & support | 2019 |
| Uni-T Associates Ltd | Other services | 2018 |
| Unicorn Securities Limited | Other services | 2021 |
| Unicorn Supported Living Limited | Real estate | 2008 |
| Unified International Accountants Ltd | Professional & scientific | 2024 |
| Unified Scholars and Publishers UK Ltd. | Information & communication | 2022 |
| Unik Granite Limited | Construction | 2024 |
| Uninetuk Ltd | Education | 2024 |
| Union Holdings Selby Limited | Financial services | 2025 |
| Union Properties Selby Limited | Real estate | 2025 |
| Union Tyre Collections Ltd | Wholesale & retail | 2024 |
| Union Tyre Recycling Ltd | Manufacturing | 2024 |
| Union Tyre Wholesale Ltd | Wholesale & retail | 2024 |
| Uniprivee Ltd | Financial services | 2016 |
| Uniq Systems Limited | Extraterritorial | 1991 |
| Uniq Traders Limited | Wholesale & retail | 2024 |
| Unique Asset Holdings Ltd | Real estate | 2026 |
| Unique Halo Limited | Professional & scientific | 2014 |
| Unique Kare Group Ltd | Health & social care | 2024 |
| Unique Oriental Limited | Hospitality | 2020 |
| Unique Portfolio Limited | Financial services | 2026 |
| Unique Projects Limited | Construction | 2019 |
| Unique Residential Properties Limited | Real estate | 2024 |
| Unique Treasures Collection Limited | Wholesale & retail | 2026 |
| Unique Uniforms Limited | Wholesale & retail | 2015 |
| Unique Utility Solutions Ltd | Professional & scientific | 2016 |
| Unique Windows (Derby) Limited | Construction | 2014 |
| United Castles Ltd | Information & communication | 2025 |
| United Centre of Excellence Limited | Education | 2017 |
| United Concrete MIX Ltd | Manufacturing | 2022 |
| United Continental Foods Limited | Manufacturing | 1986 |
| United Derby Transport Ltd | Transport & logistics | 2015 |
| United Emirates Ltd | Wholesale & retail | 2025 |
| United Family International Church (UK) | Other services | 2018 |
| United Fitness (Derby) Community Interest Company | Arts & recreation | 2025 |
| United Imperial Limited | Wholesale & retail | 2001 |
| United Staff Limited | Administrative & support | 1999 |
| Uniti Scaffolding Limited | Construction | 2017 |
| Unitize Ltd | Information & communication | 2025 |
| Unity Financial Solutions Ltd | Financial services | 2024 |
| Unity Gifts Ltd | Wholesale & retail | 2025 |
| Unity One Gym Ltd | Arts & recreation | 2023 |
| Unity Quarter (Salford) Management Company Limited | Households | 2008 |
| Universal Accountants & Tax Experts Ltd | Professional & scientific | 2026 |
| Universal Accountants Ltd | Professional & scientific | 2014 |
| Universal Commerce and Industries Limited | Administrative & support | 2013 |
| Universal Convenience Ltd | Wholesale & retail | 2025 |
| Universal Homes Limited | Construction | 2021 |
| Universal Land Limited | Construction | 2021 |
| Universal Motors Group Limited | Wholesale & retail | 2025 |
| Universal Paradise Ltd | Wholesale & retail | 2017 |
| Universal Paving UK Ltd | Wholesale & retail | 2021 |
| Universal Rainbow of Care Ltd | Health & social care | 2018 |
| Universal Signalling Ltd | Professional & scientific | 2022 |
| Universe Accountants Ltd | Professional & scientific | 2024 |
| University of Derby FE | Extraterritorial | 2012 |
| University of Derby Students' Union | Other services | 2016 |
| University of Derby Theatre Limited | Arts & recreation | 2009 |
| Uniwide Impex Limited | Wholesale & retail | 2022 |
| Unknown Properties Ltd | Real estate | 2022 |
| Unlimited Clothing Ltd | Wholesale & retail | 2024 |
| Unlimited Washing Ltd | Other services | 2025 |
| Unsullied Cleaning Services Ltd | Administrative & support | 2023 |
| Unturned Stone Limited | Real estate | 2014 |
| UP 24 Seven Services Ltd | Health & social care | 2017 |
| Upbeat Communities | Health & social care | 2005 |
| Upbeat Community Cheer and Dance CIC | Arts & recreation | 2023 |
| Upbeat Enterprises Limited | Administrative & support | 2022 |
| Upholstery Medics Limited | Other services | 2012 |
| Uplands Services Ltd | Transport & logistics | 2018 |
| Uppercrust Caterers Limited | Hospitality | 2020 |
| Upperdale Site Services Limited | Transport & logistics | 2001 |
| Upton Mechanical Services Limited | Construction | 1999 |
| Uptonshire & ST Limited | Information & communication | 2025 |
| Urban Angel Aesthetics Ltd | Other services | 2021 |
| Urban Angels Hair Boutique Limited | Other services | 2011 |
| Urban Bays Limited | Real estate | 2022 |
| Urban Bespoke Interiors Limited | Manufacturing | 2024 |
| Urban Contours Ltd | Professional & scientific | 2013 |
| Urban Custom Clothing Ltd | Wholesale & retail | 2025 |
| Urban Driveway Services Ltd | Construction | 2022 |
| Urban Dromedary Ltd | Wholesale & retail | 2021 |
| Urban Edit Ltd | Real estate | 2021 |
| Urban Gym Group Ltd | Education | 2026 |
| Urban Leisure (Wigan) Ltd | Real estate | 2025 |