| Eliizabeth Limited | Wholesale & retail | 2025 |
| Elite Fencing & Property Maintenance Ltd | Construction | 2026 |
| Elite Fleet Cleaning Limited | Transport & logistics | 2015 |
| Elite HR Limited | Professional & scientific | 2013 |
| Elite Installations Nationwide Limited | Construction | 2013 |
| Elite Motor Darlington Services Ltd | Wholesale & retail | 2026 |
| Elite Transport Compliance Ltd | Manufacturing | 2023 |
| Eliteheat & GAS Ltd | Construction | 2025 |
| Ellenor Properties Limited | Real estate | 2025 |
| Ellerslie Property Limited | Real estate | 2017 |
| Ellie Divine Limited | Health & social care | 2024 |
| Ellis Sales Ltd | Wholesale & retail | 2025 |
| ELM Integrated Services Limited | Construction | 2016 |
| Elmeniawy General Trading Ltd | Wholesale & retail | 2025 |
| Elsafty Trading Ltd | Wholesale & retail | 2025 |
| Ely's Cleaning Ltd | Administrative & support | 2020 |
| Ely's Food Trailer Ltd | Hospitality | 2021 |
| Elysian Developments U.K Ltd | Construction | 2018 |
| Elysian Haus Ltd | Health & social care | 2025 |
| EM Education Ltd | Education | 2013 |
| Embellish Darlington Ltd | Other services | 2019 |
| Ember Legal Limited | Professional & scientific | 2026 |
| EMBRACE4SUPPORT North East CIC | Professional & scientific | 2020 |
| EMC Express Ltd | Wholesale & retail | 2018 |
| Emeraude Developments Ltd | Real estate | 2022 |
| Emergency Mobile VET Ltd | Professional & scientific | 2024 |
| Emergency Roofer Limited | Construction | 2025 |
| EMF Properties Ltd | Real estate | 2026 |
| Emi's (PVT) Ltd | Hospitality | 2025 |
| Emma Hignett Ltd | Information & communication | 2006 |
| Emma Orton Education Ltd | Education | 2026 |
| Emmanuel General Services Limited | Construction | 2023 |
| Emmerson Industrial Services Limited | Manufacturing | 2014 |
| Emonda Solutions Ltd | Professional & scientific | 2024 |
| Emphasise Digital Ltd | Information & communication | 2023 |
| Emsk Risk Management Ltd | Professional & scientific | 2024 |
| Emton Enterprises Limited | Wholesale & retail | 2026 |
| En-Vision (UK) Limited | Professional & scientific | 2005 |
| Enabling Insights Ltd | Information & communication | 2018 |
| Endeavour Electrical Engineering Ltd | Construction | 2026 |
| Endeavour Property Investments Limited | Real estate | 2026 |
| Endeavour Property Partners Ltd | Real estate | 2023 |
| Endolys Holdco Ltd | Financial services | 2024 |
| Endolys Ltd | Manufacturing | 2023 |
| Enduraglaze NE Limited | Administrative & support | 2019 |
| Endustrials Ltd | Real estate | 2020 |
| Engage Mobile Limited | Information & communication | 2020 |
| Engine Shed PUB Co Limited | Hospitality | 2023 |
| England & Lyle Limited | Professional & scientific | 1997 |
| Enigma Hair Limited | Other services | 2006 |
| Enjay Taverns Limited | Hospitality | 2018 |
| Enola Homes Ltd | Real estate | 2021 |
| Enpointe Dance Company Ltd | Arts & recreation | 2021 |
| Enrico's Hair Company Limited | Other services | 2022 |
| Enterprise Design Limited | Professional & scientific | 2017 |
| Enterprise Inside Limited | Administrative & support | 2012 |
| Envensis Limited | Professional & scientific | 2011 |
| Envirolink Recycling (UK) Ltd | Professional & scientific | 2010 |
| Enviromech Engineering Ltd | Manufacturing | 2017 |
| Environmental Green Group Limited | Water & waste | 2021 |
| Environmental Green Group Properties Limited | Hospitality | 2025 |
| EOS Safety Limited | Public administration | 2025 |
| Epcot Leisure Limited | Manufacturing | 1985 |
| Epic Suite Limited | Extraterritorial | 2023 |
| EPK Bespoke Limited | Wholesale & retail | 2018 |
| Equip Bros Ltd | Wholesale & retail | 2026 |
| Equiseat AID Ltd | Wholesale & retail | 2021 |
| Equity Credit Management Limited | Other services | 2007 |
| ERA 21 Plus Limited | Other services | 2012 |
| Erion Limited | Information & communication | 1998 |
| Ernies Convenience Store Ltd | Wholesale & retail | 2025 |
| ERS NE Ltd | Construction | 2023 |
| Esenry Limited | Wholesale & retail | 2026 |
| ESG Realty Limited | Real estate | 2021 |
| Eskdale Developments Ltd | Construction | 2019 |
| Essential Law Ltd | Information & communication | 2023 |
| Essex Business Machines Ltd | Administrative & support | 2013 |
| Estelle Clare Ltd | Professional & scientific | 2011 |
| Estill Cooper Construction Limited | Construction | 1978 |
| ESW Contractors Ltd | Construction | 2021 |
| ET Turkish Barbers Ltd | Other services | 2025 |
| ETC Associates Ltd | Professional & scientific | 2013 |
| Eternal Energy Limited | Construction | 2011 |
| Eternal Stoneworks Limited | Manufacturing | 2026 |
| Ethos A&M Ltd | Professional & scientific | 2019 |
| Eton Environmental Group Ltd | Construction | 2004 |
| Etto Productions Ltd | Arts & recreation | 2021 |
| ETW Alderson Logistics Limited | Transport & logistics | 2025 |
| Euro Super Abrasives Limited | Wholesale & retail | 2013 |
| Euro Surface Tech Ltd | Construction | 2011 |
| Euro-Africa Enterprises Limited | Wholesale & retail | 2005 |
| Eurojag Group Ltd | Financial services | 2020 |
| Eurojag Limited | Wholesale & retail | 1995 |
| Eurojag Services Ltd | Wholesale & retail | 2020 |
| Euromota Limited | Transport & logistics | 2018 |
| Eurotruck Express Limited | Transport & logistics | 2024 |
| EVB Holdings Ltd | Financial services | 2020 |
| EVB1 Limited | Financial services | 2020 |
| EVE Beauty Ltd | Other services | 2011 |
| EVE Craft Your Dreams Limited | Wholesale & retail | 2023 |