| Effectivenow Ltd. | Professional & scientific | 2019 |
| Egham Estates Ltd. | Real estate | 2004 |
| EH22 Ltd | Wholesale & retail | 2018 |
| Ehire Limited | Transport & logistics | 2019 |
| EK Fitness Academy Ltd | Arts & recreation | 2024 |
| EL Property Partners Ltd | Real estate | 2023 |
| Elilenti Limited | Arts & recreation | 2021 |
| Eliock Hydro Electric Company Limited | Energy | 1991 |
| Elite Flooring Solutions Ltd | Construction | 2018 |
| Ellis Lawson Properties Ltd | Real estate | 2019 |
| ELM Lodge Estates Limited | Real estate | 1998 |
| Elmac Projects Ltd | Manufacturing | 2024 |
| Elyssa Ltd | Professional & scientific | 2020 |
| EMA Food Scotland Ltd | Wholesale & retail | 2020 |
| Ember Boutique Ltd | Construction | 2026 |
| Emerald Aesthetics & Wellbeing Ltd | Professional & scientific | 2024 |
| EMG Transfers Ltd | Transport & logistics | 2018 |
| EMI Hair Limited | Other services | 2014 |
| EMI Murdoch Limited | Other services | 2015 |
| EMI Property Scotland Ltd | Real estate | 2025 |
| EMI Stoves Limited | Other services | 2017 |
| Empire Customs Automotive Ltd | Professional & scientific | 2018 |
| EMT Taxis Limited | Transport & logistics | 2020 |
| Endorphin Shawfair Ltd | Health & social care | 2026 |
| Engage Clinical Psychology Ltd | Health & social care | 2020 |
| Eren Properties Scotland Limited | Real estate | 2021 |
| Erena Sports Limited | Arts & recreation | 2023 |
| Erskine Financial Ltd | Financial services | 2023 |
| Erskine Property Management Ltd | Real estate | 2025 |
| ESK Commercial Services Ltd | Professional & scientific | 2017 |
| Eskbank Chiropractic Clinic Limited | Health & social care | 2003 |
| Eskbank Cleaning Services Limited | Administrative & support | 2024 |
| Eskbank Design Studio Limited | Professional & scientific | 2014 |
| Eskbank Investments Ltd | Real estate | 2024 |
| Eskbank Yoga Lounge LLP | | 2019 |
| Eskstar Ltd | Professional & scientific | 2015 |
| Eskview Joiners Limited | Construction | 2021 |
| ESM Holdings (Scotland) Limited | Real estate | 2025 |
| Essa to LET Properties Ltd | Real estate | 2024 |
| Estilo Hair Ltd | Other services | 2013 |
| Estondale Properties Limited | Real estate | 1991 |
| Ethical Seafood Research Centre Ltd | Agriculture & fishing | 2025 |
| Ethical Seafood Research Ltd | Agriculture & fishing | 2021 |
| Ethicore Developments B Ltd | Construction | 2024 |
| Ethicore Developments C Ltd | Construction | 2024 |
| Ethicore Developments D Ltd | Construction | 2024 |
| Ethicore Developments Ltd | Construction | 2024 |
| Etoranj Ltd | Transport & logistics | 2023 |
| EV Solutions Ltd | Agriculture & fishing | 2019 |
| Eva's Fish Bar Edinburgh Ltd | Administrative & support | 2018 |
| Everest Rope Access and Roofing Ltd | Construction | 2023 |
| Evnt HSE Ltd | Hospitality | 2024 |
| Evolence Crest Limited | Wholesale & retail | 2026 |
| Evolve Surfacing Ltd | Construction | 2026 |
| EW Scope Ltd | Information & communication | 2023 |
| Exec Security Ltd | Administrative & support | 2021 |
| Expanding Perspectives Ltd | Other services | 2017 |
| Eyzon Energy Limited | Professional & scientific | 2017 |
| F&M Commercials Ltd | Wholesale & retail | 2026 |
| F.D.M. Taxis Ltd. | Professional & scientific | 2001 |
| FAC365 Scotland Ltd | Information & communication | 2024 |
| Facyl Consulting Ltd | Administrative & support | 2024 |
| Family Advertising Limited | Professional & scientific | 2002 |
| Far-Flung Creations Ltd | Information & communication | 2010 |
| FAST2YOU Ltd | Construction | 2012 |
| FB Access Services Ltd | Construction | 2025 |
| Ferrol Fencing and Landscaping Services Limited | Administrative & support | 2025 |
| Fertility Health Edinburgh Ltd | Health & social care | 2025 |
| Fife Retail Centre Limited | Wholesale & retail | 2020 |
| Film Cars Limited | Extraterritorial | 2015 |
| First Mont Ltd | Real estate | 2023 |
| Firstmont Transport Ltd | Transport & logistics | 2026 |
| Fisher & Anderson Ltd. | Construction | 2019 |
| Fisher Marketing Ltd | Professional & scientific | 2020 |
| Fixt Property Ltd | Real estate | 2024 |
| Flat 4 Life Ltd | Real estate | 2024 |
| Flexform Legal Services Limited | Professional & scientific | 2017 |
| Flexiclinix Ltd | Health & social care | 2025 |
| FMC Property Ltd | Real estate | 2021 |
| FN Joinery & SON Limited | Manufacturing | 2025 |
| Fonti Properties Ltd | Real estate | 2023 |
| Forth Catering and Refrigeration Engineers Limited | Other services | 1993 |
| Forthview Business Services Ltd | Professional & scientific | 2024 |
| Four Mile Trading Co. Limited | Extraterritorial | 1999 |
| Frabri Limited | Wholesale & retail | 2025 |
| Fraser Ewart Limited | Hospitality | 2026 |
| Freelance Euro Services (Mmcxcviii) Limited | Extraterritorial | 2006 |
| FW Primary Tuition and Consultancy Limited | Education | 2024 |
| G R Heating Limited | Construction | 2005 |
| G&H Lyall Ltd | Administrative & support | 2024 |
| Gareth Evans Plumbing Ltd | Construction | 2023 |
| Gaswarm Services Ltd | Construction | 2006 |
| GB Events Ltd | Hospitality | 2019 |
| GCS Building and Landscaping Ltd | Construction | 2023 |
| Gecmc Limited | Real estate | 2022 |
| Geelox Ltd | Professional & scientific | 2013 |
| Genesis (J & T) Limited | Administrative & support | 2004 |
| Genesis Property Partnership Limited | Administrative & support | 2016 |
| Genus Medical Limited | Health & social care | 2016 |
| Genus Medical Partnership Limited | Health & social care | 2018 |