| Dalgetty Services Limited | Real estate | 2015 |
| Dalkeith After School Club | Health & social care | 2002 |
| Dalkeith ALL Trades (Properties) Limited | Real estate | 2015 |
| Dalkeith ALL Trades Limited | Construction | 2010 |
| Dalkeith and District Citizens Advice Bureau | Information & communication | 2007 |
| Dalkeith Beauty Ltd | Other services | 2023 |
| Dalkeith Computers Limited | Other services | 2010 |
| Dalkeith Construction Ltd | Construction | 2014 |
| Dalkeith Dental LAB Ltd | Manufacturing | 2026 |
| Dalkeith Handy Store Ltd | Other services | 2015 |
| Dalkeith Investments Limited | Real estate | 2021 |
| Dalkeith Lawn Services Ltd | Administrative & support | 2025 |
| Dalkeith Pet & Outdoor Supplies Ltd | Wholesale & retail | 2018 |
| Dalkeith Roofing Services Limited | Construction | 2018 |
| Dalkeith Spice Ltd | Hospitality | 2025 |
| Dalkeith Thistle Community Football Club | Other services | 2003 |
| Dalrymple Taxis Ltd. | Professional & scientific | 2004 |
| Damas Edinburgh Limited | Hospitality | 2016 |
| Damasburgh Ltd | Real estate | 2023 |
| Dasfak Consulting Limited | Hospitality | 2022 |
| Datiq Ltd | Information & communication | 2026 |
| David LOW Consulting Ltd | Professional & scientific | 2017 |
| Davidson Capital Holdings Ltd | Financial services | 2025 |
| Davidson Energy Services Ltd | Construction | 2024 |
| Dawnmuir Limited | Professional & scientific | 2000 |
| DB Joinery and Carpentry Ltd | Construction | 2026 |
| DBM Plumbing and Heating Ltd | Administrative & support | 2017 |
| DE Burgh Fine Wine Limited | Wholesale & retail | 2012 |
| Deblur Ltd | Information & communication | 2016 |
| Decency Services Ltd | Administrative & support | 2026 |
| Deepqa Limited | Information & communication | 2022 |
| Delight Assets LP | | 2015 |
| Deluge Verification Limited | Professional & scientific | 2013 |
| Dentcentral Group Ltd | Wholesale & retail | 2020 |
| DG Engineering Ltd | Professional & scientific | 2025 |
| DGD Scaffolding Ltd | Construction | 2021 |
| Diamodis Interiors Ltd | Construction | 2019 |
| Diamond Installations (Scotland) Limited | Construction | 2016 |
| Diamonds Cheerleaders CIC | Arts & recreation | 2020 |
| Dick's Edinburgh Ltd | Wholesale & retail | 2012 |
| DIGITAL1ST Limited | Information & communication | 2026 |
| Dinos S.P.A Ltd | Hospitality | 2022 |
| DIS Consultancy Limited | Professional & scientific | 2014 |
| DJ Forsyth Ltd | Construction | 2026 |
| DKT Designs Ltd | Manufacturing | 2016 |
| DL Access Control Systems Limited | Information & communication | 2008 |
| Dltm Limited | Professional & scientific | 2020 |
| DMC Audio Ltd | Professional & scientific | 2023 |
| Dom-Lux Ltd | Real estate | 2024 |
| Done Toys Ltd | Wholesale & retail | 2023 |
| Double Diamond APN Solutions Ltd | Professional & scientific | 2016 |
| Double Diamond Bespoke Tax Services Ltd | Professional & scientific | 2016 |
| Double Diamond Eclipse Member Services Ltd | Professional & scientific | 2019 |
| Double Diamond Management Services Ltd. | Professional & scientific | 2019 |
| Double Diamond Tax Solutions Ltd | Financial services | 2014 |
| Douglas Strachan Ltd | Professional & scientific | 2014 |
| DPF Cleaning Edinburgh Ltd | Wholesale & retail | 2025 |
| DRFL-24 Ltd | Construction | 2026 |
| Drum Feeds Ltd | Wholesale & retail | 2020 |
| Drum Rolls Catering Ltd | Hospitality | 2025 |
| DS Electrical (Glasgow) Limited | Construction | 2018 |
| Duncaer Group Limited | Financial services | 2025 |
| Dunedin Transport Limited | Transport & logistics | 2014 |
| Duplessi Ltd | Wholesale & retail | 2025 |
| E&M Capital Partners Limited | Financial services | 2020 |
| EAI2 Consultancy Ltd | Professional & scientific | 2020 |
| East Coast (Musselburgh) Ltd | Hospitality | 2018 |
| East Coast Eateries Ltd | Hospitality | 2023 |
| East Lothian PUB Company Ltd | Hospitality | 2024 |
| East Lothian Taxis Ltd | Transport & logistics | 2021 |
| East Park Consulting Limited | Information & communication | 2023 |
| East+Inch Consulting Ltd | Information & communication | 2018 |
| EBL Antiques Ltd | Wholesale & retail | 2012 |
| Ecompuls Ltd | Wholesale & retail | 2025 |
| Ecosse Bespoke Carpentry and Joinery Ltd | Manufacturing | 2018 |
| Edenbay Limited | Transport & logistics | 2001 |
| Edge Property Partners Limited | Hospitality | 2017 |
| Edgefield Coachworks (Edinburgh) Limited | Wholesale & retail | 2008 |
| Edibnb Ltd | Administrative & support | 2023 |
| Edin Electrical Installations Ltd. | Construction | 2016 |
| Edin PHV Ltd | Wholesale & retail | 2023 |
| Edin Taxi Ltd | Transport & logistics | 2023 |
| Edinbuild Joinery & Building Services Ltd | Construction | 2021 |
| Edinburgh Booksellers' Society Limited | Other services | 1879 |
| Edinburgh Cleaning Services Limited | Construction | 2006 |
| Edinburgh Combat Challenge Ltd. | Arts & recreation | 2010 |
| Edinburgh Contracts Ltd | Administrative & support | 2022 |
| Edinburgh Design & Build Ltd | Construction | 2017 |
| Edinburgh Drivers Ltd | Transport & logistics | 2018 |
| Edinburgh Extensions & Alterations Ltd | Construction | 2020 |
| Edinburgh Extensions Limited | Construction | 2025 |
| Edinburgh Film Productions Ltd | Arts & recreation | 2019 |
| Edinburgh Flow Plumbing Limited | Construction | 2025 |
| Edinburgh GAS Ltd | Construction | 2019 |
| Edinburgh Sprint Coach Ltd | Arts & recreation | 2025 |
| Edinburgh Taxi for Hire Ltd. | Professional & scientific | 2012 |
| Ediwood Cabinet Makers Ltd | Manufacturing | 2018 |
| Edmonstone Garage Limited | Wholesale & retail | 2009 |
| Edyn Creative Ltd | Professional & scientific | 2016 |
| Efero LLP | | 2005 |