| 1634 Limited | Hospitality | 2018 |
| 1997 Properties Ltd | Real estate | 2024 |
| 2020 Mobility Limited | Wholesale & retail | 2017 |
| 227/1 High Street Ltd | Hospitality | 2023 |
| 3 Dots Deep Limited | Professional & scientific | 2018 |
| 4WATERTR Limited | Professional & scientific | 2025 |
| 5TH Gear Limited | Wholesale & retail | 2003 |
| A B Gairns Contractors Ltd | Agriculture & fishing | 2003 |
| A J Pryde (Haulage) Ltd. | Transport & logistics | 2008 |
| A Moses Surgical Limited | Health & social care | 2020 |
| A to B Car & Van Services Limited | Other services | 2010 |
| A.J Dawson Joinery Ltd | Construction | 2026 |
| A.R.T. Inspection Ltd. | Administrative & support | 2011 |
| A1 Marine Consultancy Services Ltd | Professional & scientific | 2022 |
| AAB Planning Consultancy Ltd | Professional & scientific | 2024 |
| Access Retail Limited | Wholesale & retail | 2020 |
| Achray House Hotel Ltd | Hospitality | 2019 |
| Adventura Studios Ltd. | Information & communication | 2025 |
| Aerofuel Connections Ltd | Professional & scientific | 2026 |
| Aether Developments Limited | Real estate | 2016 |
| AEW Psychology Services Limited | Health & social care | 2023 |
| Aigean Limited | Professional & scientific | 2024 |
| Aits Property Holdings Ltd | Real estate | 2025 |
| AJA Engineering Services Ltd | Professional & scientific | 2019 |
| Alexander Taylor Estate Agents Ltd. | Real estate | 2007 |
| Allandmore Limited | Construction | 2018 |
| Altech Electrical Services Ltd | Construction | 2014 |
| Amazzing Offers Ltd | Wholesale & retail | 2023 |
| AMD Barbers Ltd | Other services | 2025 |
| Ampad Ltd. | Professional & scientific | 2009 |
| Amps Catering Ltd | Hospitality | 2023 |
| Amptivo Ltd | Information & communication | 2025 |
| Ancaster Consulting Limited | Professional & scientific | 2023 |
| Andrew Macdonald Joiners & Contractors Ltd. | Construction | 2008 |
| Andrews Macphee Consulting Ltd | Professional & scientific | 2022 |
| Aniks Unique Food Limited | Wholesale & retail | 2025 |
| Annan Gallery Ltd. | Wholesale & retail | 2017 |
| Archibald Frame Limited | Real estate | 1924 |
| Ardvreck School Limited | Education | 1960 |
| Arosfield Management Ltd | Administrative & support | 2004 |
| Artney Analytics Ltd | Information & communication | 2026 |
| AS1404 Financial Consulting Ltd | Professional & scientific | 2014 |
| Asha Maya Ltd | Real estate | 2025 |
| Ashaig Consulting Ltd | Professional & scientific | 2018 |
| Asset Properties (Scotland) Ltd. | Real estate | 2018 |
| Athena Land Advisory Limited | Professional & scientific | 2024 |
| Auchingarrich Camping Ltd | Hospitality | 2025 |
| Audio Advice Limited | Wholesale & retail | 2019 |
| AZQ Retail Limited | Wholesale & retail | 2026 |
| B M Marine Ltd | Administrative & support | 2011 |
| B.Powell Commercial Ltd | Professional & scientific | 2025 |
| B1B Enterprises Limited | Other services | 2015 |
| BA Fluoropolymers Ltd | Wholesale & retail | 2018 |
| Bacon & Crerar Limited | Construction | 2021 |
| Bannisters Coffee Ltd | Hospitality | 2022 |
| Barclaycomms Ltd | Professional & scientific | 2026 |
| Beetroot and Chocolate Limited | Hospitality | 2014 |
| Bellyclone Farm | | 1992 |
| Belmont Properties Limited | Construction | 1987 |
| Bennett Galleries Limited | Wholesale & retail | 2021 |
| Bespoke Stairs Limited | Construction | 2022 |
| Best Tandoori Limited | Hospitality | 2021 |
| Bigg Design Limited | Professional & scientific | 2025 |
| Biosysmic Ltd | Professional & scientific | 2022 |
| Blairmore Farm & Estates Limited | Hospitality | 2013 |
| Blue Noun Ltd | Education | 2018 |
| Blue Swan Projects Limited | Construction | 2014 |
| Bluecrags Consulting Ltd | Professional & scientific | 2020 |
| Bonnie Boxes Ltd | Wholesale & retail | 2019 |
| Bonnie Vida Ltd | Real estate | 2025 |
| Borisbeg Ltd | Professional & scientific | 2024 |
| Borthwick Decorators Limited | Construction | 2015 |
| Breathing Journeys Ltd | Health & social care | 2015 |
| Brian Doole Golf Limited | Wholesale & retail | 2005 |
| Brian Jack & Sons Builders Limited | Construction | 2022 |
| Bridge of Ross Wood Limited | Agriculture & fishing | 2010 |
| Bridgend Bites Limited | Hospitality | 2025 |
| Brock Joinery & Construction Ltd | Construction | 2025 |
| Bsqrd Limited | Information & communication | 2022 |
| Burrotech Limited | Professional & scientific | 2002 |
| Burton Millar Limited | Professional & scientific | 1991 |
| Bushcraft Bairns Ltd | Education | 2025 |
| C Roberts Electrical Services Limited | Construction | 2022 |
| C&D Maintenance Ltd | Construction | 2023 |
| C'Est LA VIE Boutique LLP | | 2015 |
| C.B. Planning Limited | Professional & scientific | 2002 |
| Caffeine FIX Limited | Manufacturing | 2006 |
| Cairncroft Ltd. | Agriculture & fishing | 2020 |
| Caledonian Inns Limited | Hospitality | 1986 |
| Caledonian Lodges Ltd | Hospitality | 2021 |
| Cameron Process & Mechanical Engineering Ltd | Professional & scientific | 2019 |
| Camp 21 GIN Ltd | Manufacturing | 2020 |
| Camp 21 Ltd | Extraterritorial | 2020 |
| Campbell of Doune Ltd | Administrative & support | 2004 |
| Campbell Pitt Joinery Ltd | Construction | 2017 |
| Campbell-Calliva Limited | Hospitality | 2018 |
| Cardenden Premier Ltd | Wholesale & retail | 2023 |
| Casadonia Accommodation Ltd | Real estate | 2017 |
| CBL Construction Scotland Ltd | Construction | 2026 |
| Chapmans PM Ltd | Real estate | 2013 |