| Chill Perthshire CIC | Arts & recreation | 2022 |
| China City Perth Ltd | Hospitality | 2024 |
| Chipandpindirect Limited | Financial services | 2008 |
| Chiverton Engineering Limited | Professional & scientific | 2009 |
| Chocolate Galley Ltd | Administrative & support | 2015 |
| Claire Warden Limited | Education | 2012 |
| Clathic Estate Limited | Agriculture & fishing | 2025 |
| Clinical Evidence Matters Ltd | Professional & scientific | 2022 |
| Clunie Clinque Limited | Other services | 2025 |
| CMK Solutions Limited | Professional & scientific | 2002 |
| Colin Crawford Associates Ltd. | Professional & scientific | 2018 |
| Comrie Development Trust | Administrative & support | 2006 |
| Comrie Millennium Footpath Association | Health & social care | 2001 |
| Comrie Workspace Limited | Administrative & support | 2018 |
| Connectugo Ltd | Education | 2025 |
| Consolida Research Ltd | Professional & scientific | 2007 |
| Construct Architects Ltd | Professional & scientific | 2011 |
| Cooper Woodwork Limited | Construction | 2024 |
| Craig Varr Properties Limited | Real estate | 2021 |
| Cramb Construction Limited | Construction | 2024 |
| Crave-In Enterprises Limited | Hospitality | 2018 |
| Creative Crieff | Other services | 2019 |
| Crieff Community Trust | Other services | 2013 |
| Crieff Construction Limited | Construction | 2018 |
| Crieff Financial & Mortgage Centre Limited | Financial services | 2007 |
| Crieff Gardens Limited | Administrative & support | 2026 |
| Crieff Golf Club, Limited | Arts & recreation | 1913 |
| Crieff Highland Gathering Limited | Arts & recreation | 1935 |
| Crieff Hydro Limited | Hospitality | 1867 |
| Crieff Nail and Spa Limited | Other services | 2023 |
| Crieff Self Storage Ltd | Transport & logistics | 2024 |
| Crieff Sensory & Illusion Activity Centre C.I.C. | Education | 2026 |
| Crieff Wild Game Limited | Wholesale & retail | 2025 |
| Crieffchipshop Ltd | Manufacturing | 2024 |
| Crofthead Farm | | 1981 |
| Cromar's Restaurants Ltd | Hospitality | 2004 |
| Crossings House Design Ltd | Professional & scientific | 2013 |
| Culdees Castle Estate Ltd | Hospitality | 2020 |
| Cultybraggan Fitness Limited | Arts & recreation | 2023 |
| Cultybraggan Storage Limited | Real estate | 2024 |
| CYT Associates Limited | Professional & scientific | 2015 |
| D a Shanks Limited | Agriculture & fishing | 2012 |
| D Clark Consulting Limited | Professional & scientific | 2016 |
| Dave Mckay Painter & Decorator Limited | Construction | 2026 |
| Dawson & Deacon Joiners & Contractors Ltd | Construction | 2020 |
| Db's Loaded Spuds and Sandwiches Ltd | Hospitality | 2026 |
| DC Produce Direct Limited | Wholesale & retail | 2003 |
| DC Property (Scotland) Ltd | Real estate | 2023 |
| DC Restoration Contracts Ltd | Construction | 2021 |
| DE Vega Bar Development Consultants Ltd | Hospitality | 2016 |
| De-Still Creative Ltd | Administrative & support | 2018 |
| Deimatic Ltd | Professional & scientific | 2025 |
| Deli & Dough Limited | Hospitality | 2024 |
| Delivery Partners (Scotland) Limited | Other services | 2025 |
| Delivino Wild Kitchen Ltd | Hospitality | 2022 |
| Denholm Partnership (Scotland) Ltd | Professional & scientific | 2024 |
| Denholm Partnership LLP | | 2011 |
| Derek Saunders Builders Ltd | Construction | 2013 |
| Derser Ltd | Wholesale & retail | 2025 |
| Direct Rosettes (Scotland) Limited | Manufacturing | 2018 |
| DKL Consultancy Services Limited | Professional & scientific | 2022 |
| Dmsubsea Limited | Professional & scientific | 2016 |
| Donnie Delivers Ltd | Transport & logistics | 2025 |
| Dragonfly Coaching Ltd | Administrative & support | 2019 |
| Dream Tipi Limited | Arts & recreation | 2020 |
| Drumplusdrones LLP | | 2024 |
| Dunmoid Ltd | Information & communication | 2026 |
| Earnside Ltd | Agriculture & fishing | 2013 |
| Easter Tully Bannocher Farming Partnership | | 1984 |
| ECO3 Electrical Ltd | Construction | 2021 |
| Edinburgh Real ALE Limited | Hospitality | 1991 |
| Eight Trick Pony Ltd | Arts & recreation | 2023 |
| Elate Scotland Ltd | Transport & logistics | 2025 |
| Element Footwear Ltd | Wholesale & retail | 2022 |
| Elopement Buddy Ltd | Administrative & support | 2025 |
| EMS & Co Bakery Limited | Wholesale & retail | 2025 |
| Enable Finance Limited | Professional & scientific | 2023 |
| Enigma Gold Ltd | Transport & logistics | 2025 |
| EON Holistics Ltd | Wholesale & retail | 2022 |
| Estate Lives Ltd | Administrative & support | 2024 |
| Etive Ecology Limited | Real estate | 2013 |
| Ewen Stewart & SON Ltd. | Transport & logistics | 2018 |
| F&H Hairdressing Enterprises Ltd | Other services | 2019 |
| Fair Hair Ltd | Other services | 2023 |
| Fairways Catering Limited | Hospitality | 2010 |
| Family Favourite Textiles Nationwide Limited | Wholesale & retail | 1995 |
| Fault Line Fare Limited | Manufacturing | 2010 |
| FDM Ecology Limited | Professional & scientific | 2009 |
| Fermac Financial Services Ltd. | Financial services | 2005 |
| Ferntower Consulting Ltd | Professional & scientific | 2019 |
| Ferntower Holdings Ltd | Real estate | 2025 |
| Fieldwalk Holdings Limited | Real estate | 2017 |
| Fieldwalk Ltd | Hospitality | 2014 |
| Fieldwalk Management Ltd | Hospitality | 2017 |
| Fieldwalk Western Ltd | Education | 2024 |
| Findhuglen Ranges Blast Design Limited | Professional & scientific | 2025 |
| Floating Power Plant Ltd | Professional & scientific | 2015 |
| Flower Grower Collective Limited | Wholesale & retail | 2023 |
| Fmcg Technical Ltd | Professional & scientific | 2025 |
| Food Tandoori Ltd | Hospitality | 2026 |