| 185 Limited | Real estate | 2020 |
| 2707 Aesthetics Ltd | Other services | 2024 |
| A Rated Home Improvements(Fife) Ltd | Construction | 2018 |
| A Scott Stairs & Joinery Limited | Manufacturing | 2019 |
| A.F.R. (Dunfermline) Limited | Real estate | 1999 |
| Acorn Electrical Ltd. | Construction | 2015 |
| Acorn Green Energy Ltd. | Construction | 2018 |
| Activereach Ltd | Other services | 2024 |
| Adncr Ltd | Wholesale & retail | 2025 |
| Adsley Exterior Maintenance Ltd | Construction | 2026 |
| Aerora Limited | Professional & scientific | 2024 |
| AIM Food Store Ltd | Wholesale & retail | 2019 |
| Ajscstoreandfood Limited | Wholesale & retail | 2026 |
| AK7+ Limited | Construction | 2024 |
| Alan Black Fitness Limited | Arts & recreation | 2024 |
| Alan Black Nutrition Limited | Arts & recreation | 2024 |
| Alanya Turkish Ltd | Other services | 2024 |
| All-Star Cleaning Solutions Ltd | Administrative & support | 2025 |
| Amber Roofing Limited | Construction | 1991 |
| AMC Removals (UK) Limited | Transport & logistics | 2010 |
| AMC Removals and Storage Ltd | Transport & logistics | 2025 |
| Amor Construction Ltd | Construction | 2015 |
| Angus Allan Limited | Information & communication | 2019 |
| Annfield Farm Fife LLP | | 2020 |
| ANR Graceful Living Limited | Real estate | 2025 |
| Antalya Scot Ltd | Other services | 2023 |
| Arthur Place 1965 Limited | Extraterritorial | 2014 |
| Arwis Group Limited | Information & communication | 2026 |
| AS Drones Ltd | Professional & scientific | 2025 |
| Asian Cuisine Fife Ltd | Hospitality | 2025 |
| Ather Group Ltd | Transport & logistics | 2022 |
| Avantgarde Automotive Services Ltd | Transport & logistics | 2022 |
| B Hamilton Fabrication Ltd | Manufacturing | 2025 |
| B.Mcpake Joinery Ltd | Construction | 2018 |
| B2B Admin Ltd | Professional & scientific | 2016 |
| Balyo Foods Ltd | Hospitality | 2020 |
| Barbers Cross Ltd | Other services | 2022 |
| Barkers Treats Ltd | Wholesale & retail | 2025 |
| Barry Smith Installations Limited | Construction | 2016 |
| Bear Engineering Services Ltd | Professional & scientific | 2025 |
| Beathbrewing Ltd | Manufacturing | 2016 |
| Beyora Collection Ltd | Wholesale & retail | 2025 |
| Block Build Developments Ltd. | Construction | 2011 |
| Blue Energy Solutions Ltd | Mining & quarrying | 2026 |
| Bridgeway Autopoint Limited | Wholesale & retail | 1997 |
| Brikline Builders Ltd | Construction | 2025 |
| BT Bathrooms Ltd | Construction | 2026 |
| Bullseye Air Conditioning Ltd | Construction | 2023 |
| C & G Windows & Conservatories Ltd. | Construction | 2004 |
| C Lawrie Painting Services Limited | Construction | 2025 |
| C Lawrie Services Limited | Construction | 2018 |
| C Lawrie Training Services Limited | Other services | 2025 |
| C&S Mcalpine Ltd | Wholesale & retail | 2024 |
| C. & V. Carmichael Limited | Manufacturing | 1988 |
| C.P Electrical Crossgates Limited | Construction | 2022 |
| Camdean Taxis Ltd | Transport & logistics | 2026 |
| Campbell Electrical & GAS Services Ltd | Manufacturing | 2025 |
| Campbell Retail Limited | Wholesale & retail | 2021 |
| Care Swipe Technology Ltd | Information & communication | 2025 |
| Cartech 661 LLP | | 2017 |
| Castle Linton Ltd | Other services | 2018 |
| CC Joinery (Fife) Limited | Construction | 2019 |
| Choc N Cherry Ltd | Hospitality | 2021 |
| CK Bespoke Joinery Ltd | Construction | 2026 |
| CKJ Electrical Ltd | Construction | 2016 |
| Classique Flooring Limited | Wholesale & retail | 1993 |
| Cloud Vapours Ltd | Wholesale & retail | 2025 |
| Colin Sneddon Funeral Directors Ltd | Other services | 2020 |
| Collier Haulage Limited | Transport & logistics | 2006 |
| Collier Quarrying and Recycling Limited | Manufacturing | 2000 |
| Complete Engineering Solutions Scotland Ltd | Manufacturing | 2025 |
| Controlsafe Ltd. | Professional & scientific | 2017 |
| Cowden Hot Foods Ltd | Hospitality | 2025 |
| Cowdenbeath Desserts Ltd | Hospitality | 2024 |
| Cowdenbeath Mini Market Limited | Wholesale & retail | 2025 |
| Cozy Horizons Ltd | Hospitality | 2023 |
| Crafted Joinery Ltd | Manufacturing | 2025 |
| Crazy Baking Lady Ltd | Manufacturing | 2024 |
| Crossgates Community Woodland | Agriculture & fishing | 2004 |
| Crossgates GAS Services and Maintenance Limited | Construction | 2009 |
| Cscq Capital Ltd | Administrative & support | 2026 |
| Cutting Edge Carpets Fife Limited | Construction | 2018 |
| Cuttlehill Farm Energy Storage Limited | Other services | 2024 |
| David Anderson Flat Roofing Ltd | Construction | 2015 |
| Deals on Reels Limited | Wholesale & retail | 2002 |
| Delmor (Cowdenbeath) Ltd | Real estate | 2013 |
| Designvis Ltd | Professional & scientific | 2022 |
| Diamac Engineering Limited | Manufacturing | 1997 |
| Diveria Limited | Administrative & support | 2019 |
| DL Structures Ltd | Construction | 2024 |
| Docustore Ltd | Real estate | 2019 |
| DONTREPLY2025 Limited | Wholesale & retail | 2025 |
| Driftwood Holiday Lets Ltd | Real estate | 2026 |
| DRM Subsea Consultancy Ltd | Professional & scientific | 2025 |
| DSC5562 Engineering Consultant Ltd | Professional & scientific | 2024 |
| Dunfermline Fishing Limited | Wholesale & retail | 2025 |
| Dunfermline Repair Centre Ltd. | Wholesale & retail | 1996 |
| Dunlop Offshore Ltd | Construction | 2026 |
| Dunrobin Ltd | Real estate | 2023 |
| Dynamo Couriers Ltd | Transport & logistics | 2018 |