| Ecofenster Ltd | Construction | 2023 |
| Ecosse Stays Ltd | Households | 2025 |
| Eden Illumination Ltd | Manufacturing | 2009 |
| Edintax Ltd | Professional & scientific | 2018 |
| Elite Female Fitness Ltd | Arts & recreation | 2025 |
| Elite Female Studios Ltd | Arts & recreation | 2025 |
| Elliott Hospitality Ltd | Hospitality | 2022 |
| Elliott Property Services Ltd | Households | 2025 |
| Ellipsis Partners Ltd | Information & communication | 2025 |
| Estopia Engineering Ltd | Information & communication | 2026 |
| ET Automotive Ltd | Wholesale & retail | 2026 |
| Evan Industries Ltd | Wholesale & retail | 2023 |
| Ewenique Specialist Products Limited | Wholesale & retail | 2025 |
| Expert Consultants International Limited | Administrative & support | 2024 |
| Expert Stores Ltd | Wholesale & retail | 2025 |
| Fairplumb Limited | Manufacturing | 2010 |
| Ferry-Set Civils Ltd. | Construction | 2018 |
| Fife Antiques Ltd | Wholesale & retail | 2025 |
| Fife Day Care Services Limited | Health & social care | 1999 |
| Fife GAS Solutions Ltd. | Construction | 2021 |
| Fife Landscaping Ltd | Administrative & support | 2008 |
| Fife Laundry Services Ltd | Other services | 2017 |
| Fife Motorhome Centre Ltd | Wholesale & retail | 2022 |
| Fife Van Centre Limited | Wholesale & retail | 2015 |
| FM Tree Consultancy Ltd | Agriculture & fishing | 2022 |
| Forest Spirit Archers | Arts & recreation | 2014 |
| Forever Xing Sheng Limited | Hospitality | 2023 |
| Forth Valley Wholefoods Ltd | Hospitality | 2018 |
| Fyfe & Fraser Ltd | Construction | 2021 |
| G & C Mackenzie Decorating Ltd | Construction | 2020 |
| G & S Surfacing Ltd | Construction | 2024 |
| G Surveying Ltd | Professional & scientific | 2018 |
| Gamct Electrical Services Ltd | Energy | 2022 |
| Ganna Hospitality and Events Ltd | Health & social care | 2025 |
| Ghost Post Couriers Limited | Transport & logistics | 2025 |
| Girvan Taxis Limited | Extraterritorial | 2025 |
| Glenfield Autocentre Ltd | Wholesale & retail | 2016 |
| Glenfield Car Sales Limited | Wholesale & retail | 2026 |
| Glenfield Developments Limited | Real estate | 1995 |
| Glenfield Group Holdings Limited | Financial services | 2025 |
| Glenfield Training Academy (Fife) Limited | Education | 2026 |
| Glenfield Training Academy Limited | Education | 2023 |
| Go Forth Caravan Services Limited | Wholesale & retail | 2023 |
| Go Forth Motorhome Hire Limited | Administrative & support | 2020 |
| Goathill Holdings Limited | Financial services | 2022 |
| Going Ballistic Ltd | Agriculture & fishing | 2009 |
| Graham Joinery Ltd | Construction | 2010 |
| Graham Utility Solutions Ltd | Professional & scientific | 2024 |
| Grant and Fairley Flooring Limited | Wholesale & retail | 2022 |
| GSC13 Ltd | Manufacturing | 2025 |
| Harmony Fields Limited | Agriculture & fishing | 2018 |
| Highgrove Properties (Fife) Limited | Real estate | 2019 |
| Hodge & Bart Properties Ltd | Real estate | 2026 |
| Hollywood Nails Cowdenbeath Ltd | Other services | 2024 |
| Home-Start Cowdenbeath Area Ltd | Health & social care | 2005 |
| HUI Ling & Amber Limited | Hospitality | 2017 |
| Hydrocomm Contracts Limited | Information & communication | 2018 |
| Hydrollica Ltd | Professional & scientific | 2023 |
| HZR Enterprise Ltd | Hospitality | 2024 |
| IAN Sneddon Developments (Blairfordel) Limited | Construction | 2005 |
| IAN Sneddon Developments Limited | Construction | 2001 |
| Ijaz Sons Limited | Hospitality | 2025 |
| Inspire Carpentry Services Ltd | Construction | 2024 |
| IQ Safety Systems Ltd | Professional & scientific | 2024 |
| Ireland Entertainments Ltd | Arts & recreation | 2023 |
| Istanbul Hair Salon Ltd | Other services | 2025 |
| IT Inspection Limited | Professional & scientific | 2023 |
| Ivara Homes Limited | Real estate | 2026 |
| Ivoms Trade Ltd | Construction | 2024 |
| J.Aitken Marine Support Services Ltd | Manufacturing | 2025 |
| J.J Joinery and Building Services Ltd | Construction | 2020 |
| Jamie Alexander Construction Services Ltd | Construction | 2024 |
| JAY Ritchie Coaching Ltd | Arts & recreation | 2026 |
| JB PUB Limited | Hospitality | 2019 |
| JC Joinery (Fife) Ltd | Construction | 2024 |
| JDB Decorating and Property Services Ltd. | Construction | 2017 |
| Jersey Retail Ltd | Wholesale & retail | 2026 |
| JH Lighting Management Services Ltd | Construction | 2012 |
| JK1 Limited | Real estate | 2013 |
| JL Crossgate Ltd | Hospitality | 2023 |
| JL Tasty Food Ltd | Hospitality | 2025 |
| Jmlb Inspection Ltd. | Mining & quarrying | 2011 |
| JNM Transport Ltd | Transport & logistics | 2025 |
| John Brannan Joinery Ltd | Construction | 2019 |
| Jordan Valley Kirkcaldy Ltd | Hospitality | 2025 |
| JSJ Leisure Ltd | Hospitality | 2023 |
| Kaur Face & Brows Limited | Other services | 2017 |
| Kebab Mahal Ltd | Hospitality | 2023 |
| Kelly's Welders and Blacksmiths Limited | Manufacturing | 2004 |
| Kingdom Ceramics Limited | Manufacturing | 2022 |
| Kingdom Metal Finishing Limited | Manufacturing | 2007 |
| Kirstin Barnes-Forgan Laser, PMU & Beauty Limited | Other services | 2022 |
| KSB Electrical Fife Ltd | Construction | 2023 |
| KV Joinery Limited | Construction | 2026 |
| LA Casanova Limited | Hospitality | 2024 |
| Laird Mcpake Property Limited | Real estate | 2023 |
| LEM Commercial Services Ltd | Real estate | 2025 |
| Liam a Black Theatrical Ltd | Arts & recreation | 2022 |
| Lindsey Dalziel Photography Ltd | Professional & scientific | 2023 |
| LN Hospitality Limited | Real estate | 2026 |