| Stufbuf Ltd | Wholesale & retail | 2024 |
| Stuflow IT Services Limited | Information & communication | 2007 |
| Style Infusion Limited | Wholesale & retail | 2011 |
| Sublime Science Labs Ltd | Wholesale & retail | 2024 |
| Succour Limited | Professional & scientific | 2016 |
| Sudeley Drive Management Limited | Households | 2001 |
| Suffolk Archaeology Limited | Extraterritorial | 2020 |
| Summer Lake (Waters) Limited | Financial services | 2021 |
| Summer Lake Limited | Real estate | 2006 |
| Summit Gymnastics Limited | Arts & recreation | 2024 |
| Summit Self Build Management Consultants Limited | | 1989 |
| Sun New Hope Ltd | Extraterritorial | 2021 |
| Sunflowers PRE School Playgroup Limited | Education | 2024 |
| Sunkeeper Limited | Manufacturing | 2011 |
| Superb Creation UK Limited | Wholesale & retail | 2025 |
| Supermarine Music Ltd | Information & communication | 2017 |
| Supernova Ltd | Real estate | 2003 |
| Surgical Systems Limited | Professional & scientific | 1988 |
| Surviving Work Limited | Education | 2014 |
| Sustainable Waste Marketing Limited | Professional & scientific | 2007 |
| Sutherland Planning Limited | Professional & scientific | 2026 |
| Sutton Studio Limited | Professional & scientific | 2025 |
| SW6 Associates Limited | Administrative & support | 2022 |
| Swallowland Property Limited | Real estate | 2004 |
| Swerve Represents Ltd. | Professional & scientific | 2008 |
| Swift Industrial Maintenance Limited | Administrative & support | 2003 |
| Swindon Occupational Medical Services Limited | Health & social care | 2003 |
| Switch SFP Limited | Information & communication | 2018 |
| Sydney Free Saddlery Ltd | Wholesale & retail | 2011 |
| Symbiotic Marketing Limited | Professional & scientific | 2000 |
| Symphony Digital Ltd | Information & communication | 2012 |
| Symphony of Code Ltd | Information & communication | 2024 |
| Synergy Data Services Limited | Information & communication | 2012 |
| SYS Design Ltd | Professional & scientific | 2019 |
| T and T Electrical Contractors Limited | Construction | 2024 |
| T. J. Cullimore Contracting Limited | Construction | 2018 |
| T4C Associates Ltd | Professional & scientific | 2023 |
| Tablefish LLP | | 2022 |
| Tacked.Uk Ltd | Wholesale & retail | 2024 |
| Tags Consulting Ltd | Administrative & support | 2011 |
| Talbot Baines Limited | Other services | 2015 |
| Talbot Training Limited | Education | 2023 |
| Talent Sandbox Limited | Professional & scientific | 2022 |
| Talenthiregroup Limited | Administrative & support | 2020 |
| Talk Walk Grow LLP | | 2016 |
| Talking Wines Limited | Wholesale & retail | 2003 |
| Tanaz Ltd | Hospitality | 2017 |
| Tangible Design Office Ltd | Professional & scientific | 2024 |
| Tanners Solicitors LLP | | 2006 |
| Tase Holdings Ltd | Real estate | 2014 |
| Tase III Limited | Real estate | 2023 |
| Task Ceramic Tiling Ltd | Extraterritorial | 2025 |
| Taskewsolutions Limited | Administrative & support | 2014 |
| Taste for Business Ltd | Professional & scientific | 2014 |
| Taste for Digital Ltd | Professional & scientific | 2014 |
| Taste for Marketing Ltd | Professional & scientific | 2014 |
| Tasting Colours Community Interest Company | Health & social care | 2018 |
| Tavikess Ltd | Professional & scientific | 2012 |
| Taylor Aardal Estates Limited | Real estate | 2019 |
| Taylor Made Construction Gloucester Ltd | Construction | 2021 |
| Taylor Roofing Contractors Limited | Construction | 1989 |
| Tbarker Construction Ltd | Construction | 2023 |
| TCR Controls Ltd | Construction | 2019 |
| Team 4 Consulting Ltd | Professional & scientific | 2009 |
| Technical Connection Limited | Information & communication | 1996 |
| Technical Print Solutions Ltd | Manufacturing | 2013 |
| Technilogica Ltd | Information & communication | 2012 |
| Techno Systems (Interiors) Ltd | Construction | 2002 |
| Technovation Consulting Limited | Professional & scientific | 2025 |
| Teesdale Management Ltd | Construction | 2019 |
| Tektology Investments Limited | Information & communication | 2020 |
| Telecomstraining Ltd | Information & communication | 2016 |
| Temm Consulting Limited | Professional & scientific | 2014 |
| Tempo Digital Limited | Information & communication | 2011 |
| Tempus Consulting Limited | Manufacturing | 2002 |
| Terapeutica Ltd | Education | 2011 |
| Terrace Surveyors Ltd | Professional & scientific | 2024 |
| Terrett & Co Ltd | Professional & scientific | 2010 |
| Tetbury Estates Limited | Real estate | 2022 |
| Tetbury Wines Ltd | Wholesale & retail | 2020 |
| Thames Head Energy Ltd | Energy | 2022 |
| THE Admiral Talbot Limited | Hospitality | 2026 |
| THE Apprentice Training Agency Limited | Other services | 2019 |
| THE Apprenticeship Management Group Ltd | Administrative & support | 2017 |
| THE Apprenticeship Partner Ltd | Administrative & support | 2023 |
| THE Apprenticeship Training Agency Ltd | Administrative & support | 2017 |
| THE Barn Legacy Foundation | Arts & recreation | 2023 |
| THE Barn Theatre Project | Arts & recreation | 2016 |
| THE Big Yellow Tea POT Company Ltd | Real estate | 2018 |
| THE Bohemian Fairy Ltd | Information & communication | 2025 |
| THE Boiler Doctor Ltd | Construction | 2009 |
| THE Briefing Circle Limited | Professional & scientific | 2007 |
| THE Butcherbailey Partnership Limited | Professional & scientific | 2018 |
| THE Chatham Quays (Management) Limited | Real estate | 2007 |
| THE Child Academy Ltd | Education | 2019 |
| THE Churn Community Hub Ltd | Health & social care | 2006 |
| THE Cirencester Community Development Trust Ltd | Health & social care | 2012 |
| THE Cirencester Distilling Company Ltd | Manufacturing | 2025 |
| THE Codex Group Ltd | Financial services | 2022 |
| THE Content Partnership Limited | Professional & scientific | 2016 |