| Dynamic by Design Limited | Construction | 2003 |
| Dynamic Digital Partners Limited | Information & communication | 2024 |
| E-Motion Equine Therapy Limited | Agriculture & fishing | 2008 |
| E-Wealth UK Ltd | Information & communication | 2026 |
| E2M Associates Ltd | Professional & scientific | 2014 |
| EA HOW to Ltd | Other services | 2022 |
| Eaag Ltd | Agriculture & fishing | 2023 |
| Early Years Direct Limited | Wholesale & retail | 2006 |
| Early Years Direct.Com Limited | Extraterritorial | 2006 |
| Earthroot Construction Ltd | Construction | 2025 |
| Eastern Tavern Limited | Hospitality | 2023 |
| Eastington Estates, Lower Farm LLP | | 2019 |
| Eastleach Consultants Ltd | Other services | 2022 |
| Eastleach Downs Holdings Limited | Real estate | 2022 |
| Echo Communications Consultancy Limited | Administrative & support | 2013 |
| Eclectikware Ltd | Information & communication | 2003 |
| Eclipse Accounting Solutions Limited | Administrative & support | 2015 |
| Eco Chic Limited | Construction | 2005 |
| Eco Conservatory Solutions Limited | Construction | 2023 |
| Eco-Able Limited | Wholesale & retail | 2019 |
| Ecohouse Consultancy Limited | Professional & scientific | 2007 |
| Econet Pro Limited | Information & communication | 2008 |
| Ecosaveonline Ltd | Extraterritorial | 2009 |
| Ecosolve Ltd. | Other services | 1991 |
| ED Hyslop Ltd | Professional & scientific | 2008 |
| ED Psych Plus Limited | Education | 2012 |
| ED Rowe Medical Limited | Health & social care | 2020 |
| EDA Apprenticeships Plus Limited | Administrative & support | 2014 |
| EDA Learning and Development Limited | Education | 2011 |
| EDE Therapies Ltd | Health & social care | 2026 |
| Eden Enterprise Group Ltd | Real estate | 2021 |
| Education Board and Business Alliance Limited | Professional & scientific | 2023 |
| Educational Advantage Limited | Education | 2006 |
| Edward Bays Holding Ltd | Financial services | 2025 |
| Edward Bays Limited | Construction | 2008 |
| Edward Trehearne Limited | Extraterritorial | 2014 |
| Edward Trehearne Wealth Management LLP | | 2012 |
| Edward Van Thiel Limited | Construction | 2006 |
| Edwards Wealth Ltd | Professional & scientific | 2014 |
| Efields Ltd | Real estate | 2013 |
| EFS Portfolio Ltd | Construction | 2012 |
| EG Archery Limited | Arts & recreation | 2022 |
| EGM Aviation Ltd | Professional & scientific | 2026 |
| Ehedg UK & Ireland Ltd | Professional & scientific | 2020 |
| Eidur Ltd | Hospitality | 2020 |
| EL Derby Equestrian Ltd | Wholesale & retail | 2022 |
| Elearnity Limited | Extraterritorial | 2016 |
| Electra-Zoom Limited | Wholesale & retail | 2019 |
| Electracoms UK and Partners Limited | Construction | 2022 |
| Eleko Limited | Construction | 2005 |
| Eleven Bibury Ltd | Hospitality | 2021 |
| ELF Industrial Ltd | Wholesale & retail | 2013 |
| Eliora Healthcare Ltd | Health & social care | 2021 |
| Elite Health & Fitness UK Limited | Health & social care | 2004 |
| Elkstone Waste Management C.I.C. | Households | 1995 |
| Ellamy Projects Ltd | Construction | 2024 |
| Elliott-Jones Ltd | Real estate | 2025 |
| Elysiumco Ltd | Wholesale & retail | 2025 |
| EMH (1995) Limited | Households | 1995 |
| Emli Trading Ltd | Wholesale & retail | 2025 |
| Emma Bishop Racing Limited | Arts & recreation | 2020 |
| Emma Elizabeth Designs Ltd | Wholesale & retail | 2022 |
| Emma-Jane Trading Limited | Wholesale & retail | 2011 |
| Emml Pharma Ltd | Professional & scientific | 2026 |
| Emomentum Ltd | Professional & scientific | 2019 |
| Empire Beauty by Emily Ltd | Other services | 2025 |
| Empowered Eventing Limited | Agriculture & fishing | 2023 |
| Endocover Ltd | Health & social care | 2022 |
| Endsleigh Sporting Club Limited | Arts & recreation | 2022 |
| Enemy of Boredom Academy Limited | Other services | 2012 |
| Energist Group Limited | Financial services | 2018 |
| Energist UK Ltd | Professional & scientific | 2006 |
| Enlightened Enterprise Academy Ltd | Administrative & support | 2024 |
| Enso Energy Limited | Professional & scientific | 2015 |
| Entasis Holdings Limited | Financial services | 2021 |
| Enviz Group Ltd | Information & communication | 2012 |
| EP Secure Dog Walking Field Ltd | Other services | 2025 |
| Eparsons Limited | Administrative & support | 2024 |
| Epicentre Entertainment Limited | Information & communication | 2014 |
| Epsilon Polymers Limited | Extraterritorial | 2000 |
| Equal Energy Limited | Professional & scientific | 2013 |
| Equestris.Net Ltd | Financial services | 2001 |
| Equi Media (London) Limited | Professional & scientific | 2001 |
| Equi Media Limited | Professional & scientific | 1999 |
| Equilion Ltd | Other services | 2026 |
| Equine & Pet Services Ltd | Agriculture & fishing | 2004 |
| Ermin Farm Trading Co Ltd | Real estate | 2026 |
| Escape to the Cotswolds Limited | Extraterritorial | 2011 |
| Eskde Limited | Wholesale & retail | 2014 |
| Especial Ltd | Wholesale & retail | 2001 |
| Esqm Consulting Ltd | Professional & scientific | 2019 |
| Essenza DI Romagna Limited | Wholesale & retail | 2016 |
| Etrius Ltd | Information & communication | 2025 |
| ETS (Resources) Limited | Professional & scientific | 1990 |
| ETS Technical Selection Ltd | Administrative & support | 2007 |
| Eurax Limited | Wholesale & retail | 1999 |
| European Quantum Limited | Manufacturing | 2021 |
| Eurostore Holdings Limited | Administrative & support | 1999 |
| Event Line Ltd | Manufacturing | 2018 |
| Everys Financial Services Limited | Financial services | 2023 |