| S & M Alexiou LLP | | 2020 |
| S D Launchbury Holdings Limited | Financial services | 2017 |
| SAE Design Consultants Limited | Construction | 2019 |
| Salt Technology Services Ltd | Information & communication | 2026 |
| SAM (2000) Ltd | Financial services | 2000 |
| SAM Wilson Studio Limited | Wholesale & retail | 2015 |
| Sandstone Property Management Ltd | Real estate | 2014 |
| Santalberto Vintners Limited | Agriculture & fishing | 2003 |
| Scandinavian Skincare Systems LLP | | 2017 |
| SCOTT89 Ltd | Hospitality | 2023 |
| SDL Biomass Limited | Agriculture & fishing | 2011 |
| SDL Esco Limited | Professional & scientific | 2017 |
| SDL Pellets Limited | Manufacturing | 2022 |
| Secret Sites Limited | Hospitality | 2019 |
| Secure F I Services Limited | Other services | 2020 |
| Secure Services Limited | Professional & scientific | 1984 |
| Sendsational Services Ltd | Professional & scientific | 2026 |
| Sentratech Systems Ltd | Information & communication | 2025 |
| SFM Training Ltd | Education | 2026 |
| Signalise Ltd | Information & communication | 2026 |
| Signed and Designed Limited | Wholesale & retail | 2010 |
| Siminetti Limited | Wholesale & retail | 2010 |
| SIR Baptist Hicks Almshouses Trustee | Professional & scientific | 2013 |
| Skillstone Insulation Ltd | Construction | 2010 |
| SOU Limited | Real estate | 1998 |
| Soulistic Rush Travelling Tattoo Emporium Ltd | Other services | 2025 |
| Soutien AUX Athletes Selectionnes DE JNS Netball France Ltd | Arts & recreation | 2026 |
| Spaceship (Automotive) Ltd | Wholesale & retail | 2024 |
| Spirine Limited | Construction | 2021 |
| Spittle Farm Campsite Ltd | Hospitality | 2026 |
| ST James Court RTM Company Limited | Households | 2020 |
| Stanford Carpentry Ltd | Construction | 2024 |
| Stanhope Dental Laboratory Ltd | Health & social care | 2022 |
| Stanley's Biomass Limited | Manufacturing | 2016 |
| Startup and Scale Limited | Administrative & support | 2025 |
| Stokes Home Improvements Ltd | Construction | 2024 |
| Stratadigital Consulting Limited | Information & communication | 2026 |
| Stratford Asset Management Ltd | Financial services | 2000 |
| Stratford Car Body Repair Co. Limited | Real estate | 1963 |
| Stratford Family Mediation Ltd | Professional & scientific | 2020 |
| Strathclyde Consulting Ltd | Information & communication | 2025 |
| Studio 113 Rehabilitation Pilates Limited | Arts & recreation | 2024 |
| Studio William Welch Limited | Other services | 2001 |
| SWB Telecoms Limited | Information & communication | 2026 |
| Swift Mixer Hire Limited | Administrative & support | 2006 |
| Sørensen Crew Limited | Wholesale & retail | 2023 |
| Task HR Ltd | Administrative & support | 2022 |
| Teach ME Digital Limited | Education | 2025 |
| Teamtalk Safety Ltd | Construction | 2013 |
| Tempstor Limited | Construction | 1998 |
| THE Bakehouse Flat Ltd | Hospitality | 2025 |
| THE Bakers Arms Broad Campden Limited | Hospitality | 2024 |
| THE Bodybarn (Ebrington) Ltd | Arts & recreation | 2018 |
| THE Cotswold MAP Company Limited | Wholesale & retail | 2022 |
| THE Glasshouse Agency Ltd | Professional & scientific | 2025 |
| THE Inverter Drive Supermarket Limited | Wholesale & retail | 2006 |
| THE Pudding Club Limited | Wholesale & retail | 2009 |
| THE School of Effectiveness Limited | Professional & scientific | 2025 |
| THE Specialist Honey Company Ltd | Agriculture & fishing | 2016 |
| THE Wavey Daveys Limited | Financial services | 2026 |
| Thecarhaus Ltd | Wholesale & retail | 2023 |
| Tiburcias Ltd | Wholesale & retail | 2020 |
| Tiger Miniatures Ltd | Manufacturing | 2025 |
| TIM Horton Education Ltd | Education | 2024 |
| Timothybee Ltd | Health & social care | 2011 |
| Tiny Paws Boutique Limited | Wholesale & retail | 2025 |
| Tmje Moreton Limited | Real estate | 2014 |
| Together Cotswolds CIC | Other services | 2025 |
| Town & Country Property Management Limited | Real estate | 2020 |
| Trident Tree Solutions Ltd | Administrative & support | 2025 |
| Ulajo Ltd | Professional & scientific | 2006 |
| USP Vehicles Limited | Wholesale & retail | 2006 |
| Venomite Ltd | Professional & scientific | 2024 |
| Vilasa Limited | Wholesale & retail | 2019 |
| Vine House Consultancy Limited | Professional & scientific | 2018 |
| Vita DE LA Flora Ltd | Wholesale & retail | 2026 |
| Volcano Publishing Limited | Wholesale & retail | 2024 |
| W.R. Haines (Leasow Farms) Limited | Agriculture & fishing | 1973 |
| Walnut Acoustics Ltd | Professional & scientific | 2020 |
| Warth Developments Ltd | Extraterritorial | 2012 |
| Warth Property Holdings Limited | Construction | 1993 |
| Warwickshire Living Ltd | Real estate | 2018 |
| Website Services Limited | Information & communication | 1998 |
| Weston Edgeworks Ltd | Information & communication | 2026 |
| Wild Nest Cotswolds Limited | Wholesale & retail | 2026 |
| Wilde Studios Ltd | Professional & scientific | 2023 |
| William Welch Design Limited | Professional & scientific | 2006 |
| Woolmarket House Hospitality Limited | Hospitality | 2018 |
| WS Gabb Limited | Agriculture & fishing | 2013 |
| WSE Heritage CIC | Arts & recreation | 2025 |
| YOU LIN Ltd | Hospitality | 2025 |
| Zebra Realisations LLP | | 2008 |
| Zulu Realisations Limited | Professional & scientific | 1999 |
| Zusetsu Ltd | Wholesale & retail | 2019 |
| ZZ Topco Limited | Financial services | 2024 |