| Lomas Tree Care Ltd | Agriculture & fishing | 2025 |
| LOV Cotswold Ltd | Wholesale & retail | 2025 |
| Lucid Stay Ltd | Hospitality | 2024 |
| M Hancock Services Ltd | Professional & scientific | 2023 |
| M&S Alexiou Limited | Hospitality | 2022 |
| Made to Measure Mats Limited | Wholesale & retail | 2018 |
| Maharajah @ Volunteer Inn Ltd | Hospitality | 2019 |
| Maharajah at Camden Ltd | Hospitality | 2026 |
| Mancave Components Ltd | Wholesale & retail | 2004 |
| Maple House Company (Chipping Campden) Ltd. | Hospitality | 2020 |
| Mark Kirkbride and Company Limited | Professional & scientific | 1996 |
| Mark Williams Law Consultancy Limited | Health & social care | 2023 |
| Marlin Financial Ltd | Financial services | 2023 |
| Marville Properties Limited | Real estate | 1985 |
| Medicines Development & Licensing Ltd | Professional & scientific | 2005 |
| Medicure Professionals Limited | Administrative & support | 2013 |
| Mesa Growth Ltd | Professional & scientific | 2024 |
| Metislands Ltd | Professional & scientific | 2025 |
| Michaels Restaurant Limited | Hospitality | 2018 |
| Mickleton Bramble Limited | Real estate | 2021 |
| Mickleton Harborlow Limited | Real estate | 2021 |
| Mickleton Hills Farm Cottages Ltd | Hospitality | 2021 |
| Mickleton Hills Farm Limited | Real estate | 2017 |
| Mickleton House Management Limited | Households | 2006 |
| Mickleton Limited | Extraterritorial | 2022 |
| Mickleton Russet Limited | Real estate | 2021 |
| Mickleton Victoria Limited | Real estate | 2022 |
| Middlewest Park Management Company Ltd | Other services | 2017 |
| Midpark Capital Limited | Real estate | 2011 |
| Midpark Retreats Limited | Real estate | 2016 |
| Millian Construction Limited | Construction | 2012 |
| Milne Group Ltd | Construction | 2006 |
| Milton Consulting Ltd | Extraterritorial | 1991 |
| MLD Civil Engineering Ltd | Construction | 2016 |
| Mnemosyne Orbis Limited | Information & communication | 2026 |
| MO Academy Ltd | Education | 2018 |
| Modena Buildings Limited | Construction | 1995 |
| Montrose Commercial Limited | Construction | 2017 |
| Montrose Group (Cotswold Builders) Limited | Construction | 2009 |
| Montrose Homes Limited | Construction | 2017 |
| Moonstone Rhythm Ltd | Information & communication | 2024 |
| MOR Bakery Ltd | Manufacturing | 2019 |
| Mortlake Law Ltd | Administrative & support | 2019 |
| Mpac Management Limited | Extraterritorial | 2013 |
| MTW Holdings Ltd | Financial services | 2025 |
| Need a Gardener? Ltd | Administrative & support | 2009 |
| Netbuys Limited | Wholesale & retail | 2000 |
| Nicholas James Consultancy Limited | Professional & scientific | 2023 |
| NIH Consultancy Limited | Professional & scientific | 2025 |
| Noel Court Management Company Limited | Real estate | 2003 |
| Nook Property Group Limited | Real estate | 2021 |
| North Cotswold Schools Federation MAT | Education | 2022 |
| NRG Securities Limited | Construction | 2017 |
| Nutrition Matters Limited | Education | 1999 |
| Nuttall Business Consulting Limited | Professional & scientific | 2016 |
| Old George Properties Limited | Real estate | 1999 |
| OMG (Mickleton) Limited | Households | 2017 |
| Onecall Business Turnaround Ltd | Professional & scientific | 2021 |
| Onecall Management Limited | Hospitality | 2012 |
| Orchard Developments | | 1998 |
| Organised & Inspired Homes Limited | Wholesale & retail | 2024 |
| Outline Displays Limited | Manufacturing | 1996 |
| Overbrook Automotive T/A Hyper Car Ltd | Wholesale & retail | 2014 |
| Overtown Limited | Real estate | 2025 |
| P H Bocock Limited | Construction | 2012 |
| Paraguay Parana Plate Investment Company Limited | Administrative & support | 2024 |
| Park Road Properties Company Limited | Real estate | 1956 |
| Patchwork Hare Limited | Manufacturing | 2020 |
| Pearl Investments (Solihull) Limited | Real estate | 2000 |
| Pearlsri Scape UK Ltd | Administrative & support | 2026 |
| Peridot Digital Limited | Wholesale & retail | 2016 |
| Peridot Technology Limited | Wholesale & retail | 2019 |
| Porta Investment Ltd | Real estate | 2021 |
| Process Intelligence Limited | Information & communication | 1987 |
| Produce Holdings Limited | Real estate | 2007 |
| Protea Street Ltd | Professional & scientific | 2022 |
| Proteus Associates (Oxford) Limited | Information & communication | 2016 |
| PTH Advisory Limited | Professional & scientific | 2025 |
| Pyments of Campden Limited | Construction | 1979 |
| Quartz Group Ltd | Extraterritorial | 2005 |
| Quartz Land & Acquisitions Ltd | Real estate | 2006 |
| Quartz Property and Estates Ltd | Wholesale & retail | 2005 |
| R C C Construction Ltd | Construction | 2004 |
| R Milligan Medical Consulting Limited | Information & communication | 2018 |
| R&R ( Gloucester ) Ltd | Hospitality | 2018 |
| Raisbeck & Reason (Cheltenham) Ltd | Arts & recreation | 2006 |
| RAUNDS1 Ltd | Real estate | 2008 |
| Really Organised Ltd | Administrative & support | 2014 |
| Redcircle Residents Association Limited | Other services | 1982 |
| Relocations Experts Ltd | Hospitality | 2024 |
| Restore Clinical Ltd | Health & social care | 2025 |
| Reuse & Recycle Estate Services Ltd | Water & waste | 2026 |
| Revival Training Ltd | Arts & recreation | 2025 |
| Robert Welch Designs Limited | Manufacturing | 1961 |
| Robert Welch Limited | Extraterritorial | 2010 |
| Robert Welch Tableware Limited | Extraterritorial | 1997 |
| Rozi Advisors Ltd | Administrative & support | 2025 |
| RSR Motor Racing Limited | Arts & recreation | 2007 |
| RTG (Services) Ltd | Professional & scientific | 2014 |
| Rupert Ward Consulting Ltd | Professional & scientific | 2026 |