| E B Sealants Ltd | Construction | 2026 |
| E Hall Holdings Limited | Information & communication | 2024 |
| E Sweet Properties Limited | Real estate | 2019 |
| E W Contracting Limited | Agriculture & fishing | 1997 |
| E. and S. Cotton Limited | Financial services | 1971 |
| E2 Holdings Ltd | Wholesale & retail | 2021 |
| Early Bird Productions Ltd | Information & communication | 2024 |
| Earth Building UK and Ireland Ltd | Education | 2009 |
| Earth EYE Technology Limited | Professional & scientific | 2024 |
| Earthcare Garden Design Limited | Administrative & support | 2018 |
| Earthstone Construction Ltd | Construction | 2020 |
| Earz Research Limited | Professional & scientific | 2013 |
| Eastcombe Barn Limited | Extraterritorial | 2022 |
| Eastknook Farms Ltd | Agriculture & fishing | 2023 |
| EAT PR Ltd | Professional & scientific | 2021 |
| EAW Ventures Ltd | Information & communication | 2021 |
| Ebblo UK Limited | Information & communication | 2009 |
| EC Sustainable Limited | Professional & scientific | 2012 |
| Eco Adventure Projects Ltd | Administrative & support | 2022 |
| Eco Stars (PTY) Ltd | Professional & scientific | 2026 |
| Ecobot Limited | Information & communication | 2025 |
| Ecologiq Limited | Wholesale & retail | 2020 |
| Ecomerchant Limited | Extraterritorial | 2001 |
| Ecomerchant Natural Building Materials Limited | Wholesale & retail | 2013 |
| Ecopowersoft Limited | Information & communication | 2017 |
| Ecoroofing Systems Limited | Wholesale & retail | 2010 |
| Edena Ltd | Health & social care | 2023 |
| Edge Strategic Marketing Limited | Professional & scientific | 2008 |
| Edmund Brumfitt Rare Books Ltd | Wholesale & retail | 2022 |
| Edward Makepeace Building Ltd | Construction | 2022 |
| Edybya Ltd | Transport & logistics | 2021 |
| EGG 'N' OGG Storage Ltd | Real estate | 2023 |
| EGS Protrade Consulting Ltd | Professional & scientific | 2020 |
| Eight Cubed HR Ltd | Information & communication | 2010 |
| Eight TEN Thirteen Ltd | Construction | 2020 |
| Eighteen Aesthetics Limited | Other services | 2025 |
| EK Maternity Software Limited | Information & communication | 2013 |
| EKP Consultancy Ltd | Professional & scientific | 2016 |
| EKS Affinity Limited | Professional & scientific | 2017 |
| Elacharlotte Ltd | Other services | 2026 |
| Eladen Consulting Ltd | Administrative & support | 2016 |
| Elaia Rose Ltd | Wholesale & retail | 2024 |
| Elastic Cafnp Manager Holdings Ltd | Information & communication | 2023 |
| Elastic Cafnp Manager Ltd | Information & communication | 2023 |
| Elastic Mint Ltd | Information & communication | 2018 |
| Electrical Innovations (South West) Limited | Energy | 2006 |
| Electromation Limited | Construction | 1997 |
| Elegance Renovations Ltd | Construction | 2026 |
| Elemental Energy Technologies Ltd | Professional & scientific | 2021 |
| Elements Plumbing Ltd | Construction | 2020 |
| Elevance Digital Ltd | Information & communication | 2025 |
| Elevate Cloud Ltd | Information & communication | 2023 |
| Eleve Limited | Wholesale & retail | 2024 |
| Eleven Tenths Limited | Professional & scientific | 2022 |
| Eliana 2019 Ltd | Information & communication | 2018 |
| Elige Limited | Hospitality | 2020 |
| Elite Martial Arts Professionals Limited | Arts & recreation | 2013 |
| Eliza Filby Associates Ltd | Professional & scientific | 2017 |
| Ellendale Environmental Limited | Professional & scientific | 2010 |
| Ellerdine Developments Ltd | Construction | 2016 |
| ELM Office Holdings Limited | Financial services | 2016 |
| ELM Tree Caravan Park Limited | Hospitality | 2003 |
| ELM Workspace Ltd | Wholesale & retail | 2017 |
| Elmsgate Holdings Limited | Financial services | 2017 |
| Elpradas Hair & Nails Limited | Other services | 2010 |
| ELS Payroll Associates Ltd | Professional & scientific | 2023 |
| Elsewhere Coffee Ltd | Manufacturing | 2024 |
| Elsie Agnew Design Ltd | Arts & recreation | 2022 |
| Elward Estates Limited | Real estate | 2021 |
| EM Content Ltd | Other services | 2026 |
| Em-Hr Limited | Administrative & support | 2002 |
| Embedded Micro Technology Limited | Manufacturing | 2011 |
| Emberz Ltd | Agriculture & fishing | 2024 |
| Emery Crane Services Ltd | Manufacturing | 2024 |
| Emery Holdings South West Limited | Professional & scientific | 2021 |
| Emery Plastering Ltd | Construction | 2026 |
| Emery Trading Limited | Real estate | 2006 |
| Emily Seal Limited | Education | 2023 |
| Emmaland Properties Limited | Construction | 1971 |
| Emmanuel Holdings Limited | Professional & scientific | 2006 |
| Emmanuel Products Limited | Wholesale & retail | 1998 |
| Empire Construction & Carpentry Limited | Construction | 2019 |
| Empire Resin Limited | Wholesale & retail | 2024 |
| Empow Creative Limited | Professional & scientific | 2025 |
| Empower Energy Limited | Construction | 2010 |
| Empowherpath Limited | Professional & scientific | 2025 |
| EMS Properties Ltd | Real estate | 2023 |
| Enceladus Systems Limited | Information & communication | 2017 |
| Enchanted Mill and Pond House Ltd | Hospitality | 2023 |
| Energy Context Ltd | Professional & scientific | 2023 |
| Engage Technical Solutions Holdings Limited | Financial services | 2022 |
| Engage Technical Solutions Ltd | Public administration | 2011 |
| Engage Technical Solutions Trustee Limited | Professional & scientific | 2022 |
| Engineering Synthesis Consulting Ltd | Professional & scientific | 2014 |
| Enter Luxury Ltd | Real estate | 2024 |
| Enterprise Property Associates Ltd | Real estate | 2025 |
| Entwine Design Ltd | Professional & scientific | 2025 |
| Epictetus Ltd | Construction | 2023 |
| Epin Investments Ltd | Real estate | 2024 |
| Epsi Ltd | Information & communication | 2018 |