| Eternal Skincare Limited | Extraterritorial | 2020 |
| Etherton Property Group Ltd | Financial services | 2023 |
| Etherton Property Ltd | Real estate | 2023 |
| Eton 180 Ltd | Real estate | 2021 |
| Euro Elite Limited | Other services | 2014 |
| European Centre for Public Affairs | Professional & scientific | 1988 |
| Europromas Limited | Professional & scientific | 2018 |
| Evangelical Community Ltd | Other services | 2019 |
| Evans Weir Limited | Professional & scientific | 2007 |
| Event Games Limited | Arts & recreation | 2024 |
| Event Glue Ltd | Professional & scientific | 2022 |
| Eventflow Technologies Ltd | Information & communication | 2024 |
| Evergreen Endurance Runner Ltd | Arts & recreation | 2025 |
| Evergreen South Freeholds Ltd | Real estate | 2021 |
| Evergreen South Holdings Ltd | Financial services | 2021 |
| Evergreen South Investments Ltd | Real estate | 2021 |
| Evergreen South Properties Ltd | Real estate | 2019 |
| Evergreen South SA Ltd | Real estate | 2022 |
| Everson Associates Limited | Professional & scientific | 2009 |
| Everyday Recruitment Agency Limited | Administrative & support | 2000 |
| Evident Private Office Ltd | Professional & scientific | 2025 |
| Evident Wealth Ltd | Financial services | 2025 |
| Evolution Capital Advisors Limited | Professional & scientific | 2020 |
| Evolution Capital Holdings Limited | Professional & scientific | 2011 |
| Evolution Capital Intelligence Limited | Professional & scientific | 2018 |
| Evolution Capital M & a Limited | Professional & scientific | 2020 |
| Evoplex Limited | Real estate | 2017 |
| Evuna Legal Consultancy Ltd | Professional & scientific | 2026 |
| EWA Investments Limited | Other services | 2018 |
| Ewavelength Ltd | Information & communication | 2011 |
| Excel Clinical Limited | Professional & scientific | 2017 |
| Exchange Tunnels Limited | Financial services | 2021 |
| Exclusive Private Villas Limited | Administrative & support | 2017 |
| Executive Refurbishing Limited | Construction | 1985 |
| Exemplar Equine Limited | Agriculture & fishing | 2011 |
| Expat Travel Services Limited | Information & communication | 2014 |
| Experientially Limited | Professional & scientific | 2014 |
| Expert Home Improvements Ltd | Construction | 2025 |
| Explore Creative Ltd | Professional & scientific | 2016 |
| Expro Consultancy Group Limited | Other services | 2011 |
| Exspecto Limited | Financial services | 2020 |
| EXTRAPUSH4U Ltd | Professional & scientific | 2025 |
| Extreme Balance Limited | Information & communication | 2021 |
| Eylul & Nisa Ltd | Hospitality | 2018 |
| Ezma Property Investments Limited | Real estate | 2020 |
| F G Speed Cabins Ltd | Wholesale & retail | 2020 |
| F.A Irwin-Brown Security Ltd | Administrative & support | 2025 |
| F.W.&.R.Shrubb Limited | Agriculture & fishing | 1956 |
| FAB & ELL Limited | Information & communication | 2023 |
| Fabristick Limited | Professional & scientific | 2014 |
| Fabritec (Engineering Solutions) Limited | Professional & scientific | 2020 |
| Fabrizo Limited | Construction | 2011 |
| Fairacre Lettings Limited | Real estate | 2016 |
| Fairacre Property Developments Ltd | Construction | 2011 |
| Fairhaven (Kings Avenue) Management Company Limited | Households | 2012 |
| Fairlands Properties Limited | Real estate | 2016 |
| Fairpay Construction Ltd | Administrative & support | 2005 |
| Fairview Wines Limited | Wholesale & retail | 2015 |
| Fairway Consulting Ltd | Professional & scientific | 2019 |
| Fairways to Lawns Ltd | Administrative & support | 2024 |
| Falcon Flight Training Ltd | Other services | 2011 |
| Falcon Homes (Sussex) Limited | Construction | 1997 |
| Falcon Solar Limited | Real estate | 2011 |
| Fallon Properties Limited | Real estate | 1997 |
| Fallows (Valuation) Surveyors LLP | | 2025 |
| Family 0507 Ltd | Hospitality | 2022 |
| Family Dental Clinic Ltd | Health & social care | 2015 |
| Fandango Digital Ltd | Professional & scientific | 2016 |
| Fandorin Marketing Services Limited | Professional & scientific | 2026 |
| Fanny Adams Limited | Wholesale & retail | 2013 |
| Faragate Limited | Construction | 1997 |
| Farbridge Event Management Limited | Hospitality | 2014 |
| Farlow Solar Systems Ltd | Construction | 2025 |
| Farm Road 1952 Limited | Extraterritorial | 2014 |
| Farmers Gold Limited | Real estate | 2018 |
| Farmex Limited | Professional & scientific | 2015 |
| Farmyard Wine Ltd | Hospitality | 2018 |
| Farnah Properties Limited | Real estate | 2024 |
| Farndell Developments Ltd | Construction | 2025 |
| Farnell Consulting Limited | Administrative & support | 2025 |
| Farquhar Design Ltd | Administrative & support | 2021 |
| Farrington Management Company Limited | Households | 2016 |
| Fashion Addition Ltd | Wholesale & retail | 2026 |
| Fast Oak Ltd | Construction | 2017 |
| Fast Track Fire Suppression Ltd | Professional & scientific | 2021 |
| Fast Track Health and Beauty Ltd | Wholesale & retail | 2016 |
| Faster Construction Solutions Ltd | Construction | 2013 |
| Fastnet Securities | Real estate | 1963 |
| FAT LIP Productions Ltd | Arts & recreation | 2024 |
| Fayylix Ltd | Wholesale & retail | 2025 |
| FB Contracts Limited | Construction | 2022 |
| FCL Science Ltd | Wholesale & retail | 2008 |
| FE Sussex | Education | 2005 |
| Fearntree Joinery Ltd | Construction | 2020 |
| Fears Creative Partnerships Ltd | Health & social care | 2018 |
| FEE Management and Consultancy Ltd | Professional & scientific | 2007 |
| FEED4WARD Investment Company Limited | Administrative & support | 2021 |
| Feel Good Films Ltd | Information & communication | 2020 |
| Felicity in Birth Limited | Other services | 2025 |
| Fencol Ltd | Professional & scientific | 2016 |