| EXPEDITION279 Ltd | Transport & logistics | 2018 |
| Express Coatings Limited | Manufacturing | 2008 |
| Express Logistics (North) Limited | Transport & logistics | 2016 |
| Express of Chesterfield Limited | Transport & logistics | 2018 |
| Exvac Equipment Sales Limited | Wholesale & retail | 2023 |
| Eyam Domiciliary Service Ltd | Health & social care | 2003 |
| EYE Candy Academy Ltd | Wholesale & retail | 2023 |
| EYE Candy Salon Ltd | Other services | 2023 |
| Eyesiteview Ltd | Information & communication | 2025 |
| Eyre & Elliston Group Services Limited | Extraterritorial | 1975 |
| Eyre & Elliston Holdings Limited | Administrative & support | 1974 |
| Eyre Services Ltd | Information & communication | 2015 |
| F a W Limited | Real estate | 1980 |
| F and H Sinnington Limited | Hospitality | 2019 |
| F B S Express Ltd | Wholesale & retail | 1997 |
| F X 4 Limited | Information & communication | 2002 |
| F&L Hospitality Ltd | Hospitality | 2024 |
| F. & M. Steed Limited | Manufacturing | 1965 |
| F. G. Sissons (Chesterfield) Limited | Construction | 1983 |
| F.A.S.T Detailing Limited | Wholesale & retail | 2025 |
| F.Hall (Gunmakers) Ltd. | Manufacturing | 1961 |
| F.S Civils Ltd | Construction | 2023 |
| F.W.D Fabrication & Welding Developments Ltd | Manufacturing | 2024 |
| F13 Limited | Information & communication | 2026 |
| Fa-St Filtration Analysis Services Technology Ltd | Mining & quarrying | 2005 |
| Fabbullies Ltd | Agriculture & fishing | 2020 |
| Face Structures Limited | Construction | 2007 |
| Faceit Aesthetics & Beauty Limited | Other services | 2017 |
| Facial Surgery UK Limited | Health & social care | 2015 |
| Facilities Management (Derbyshire) Limited | Information & communication | 2014 |
| Fadek Ltd | Wholesale & retail | 2025 |
| Fair Deal Clowne Ltd | Wholesale & retail | 2023 |
| Fairford House Trading Ltd | Other services | 2019 |
| Fairplay | Health & social care | 2009 |
| Fairytales Limited | Health & social care | 2004 |
| Faith Publishers Ltd | Information & communication | 2024 |
| Faith's Y2K Fantasy Limited | Wholesale & retail | 2025 |
| Falcon Alliance Limited | Financial services | 1998 |
| Falcon Security and Maintenance Ltd | Administrative & support | 2023 |
| Falcon Yard Limited | Health & social care | 2017 |
| Fall Protection Holdings Ltd | Financial services | 2025 |
| Fallgate Consulting Ltd | Professional & scientific | 2025 |
| Fallon Wealth Managers Limited | Financial services | 2015 |
| Family Funeral Directors Limited | Other services | 2015 |
| Famm Properties Limited | Real estate | 2015 |
| Famous Facility Resources Ltd | Administrative & support | 2022 |
| Famous Digital Resources Ltd | Wholesale & retail | 2025 |
| Fancy Nails 68 Limited | Other services | 2025 |
| Fandangles Ltd | Wholesale & retail | 2023 |
| Fanes Chesterfield Ltd | Professional & scientific | 2025 |
| FAR East Developments Limited | Real estate | 2002 |
| FAR East Europe Limited | Manufacturing | 2002 |
| FAR East London Ltd | Manufacturing | 1989 |
| Farewell Funerals Ltd | Other services | 2016 |
| Farriess Court Limited | Health & social care | 2005 |
| Fast FWD Advertising Ltd | Information & communication | 2022 |
| Fastlanetech Ltd | Information & communication | 2025 |
| FAT PIG BBQ Ltd | Hospitality | 2025 |
| Fatigue Free Futures | Health & social care | 2022 |
| Fawbert Adams Limited | Professional & scientific | 2010 |
| Fawkes Recycling Ltd | Water & waste | 2010 |
| Faye K Face Ltd | Other services | 2023 |
| Faye Knox Ltd | Wholesale & retail | 2025 |
| FC Sports Management Consultants Limited | Professional & scientific | 2009 |
| FD Academy of Aesthetics Limited | Other services | 2025 |
| Fearless Sports Ltd | Manufacturing | 2023 |
| Fearn Management Ltd | Wholesale & retail | 2011 |
| Feasby Holdings Ltd | Real estate | 2025 |
| Feather and Fern Ltd | Other services | 2025 |
| Feel Good Incorporated Ltd | Real estate | 2023 |
| Felizo PTV Limited | Health & social care | 2016 |
| Fellhelm Ltd | Information & communication | 2026 |
| Fence Webb LLP | | 2023 |
| Fenwick Engineering Technology Limited | Professional & scientific | 1986 |
| Ferdinand Bilstein UK Limited | Wholesale & retail | 1988 |
| Fermiq Limited | Professional & scientific | 2024 |
| Ferrinho Ltd | Extraterritorial | 2018 |
| Ferro Financial Ltd | Financial services | 2020 |
| Ferromatik UK Limited | Wholesale & retail | 2007 |
| Ferrovest Holdings Limited | Administrative & support | 2025 |
| Ferrovest Limited | Administrative & support | 2020 |
| Fertility Health Ltd | Professional & scientific | 2021 |
| Festival Staff Ltd | Extraterritorial | 2022 |
| Festivall Staff Ltd | Extraterritorial | 2021 |
| Festivapp Limited | Extraterritorial | 2023 |
| FHS Derbyshire Limited | Health & social care | 2020 |
| Fibre Splicing Solutions Ltd | Information & communication | 2023 |
| Fibre TEC Civils Limited | Construction | 2022 |
| Fibre TEC Group Ltd | Financial services | 2021 |
| Fibre TEC Solutions Limited | Information & communication | 2020 |
| Fibreflight Ltd | Professional & scientific | 2003 |
| Field of Freedom Ltd | Other services | 2020 |
| Fieldbay Limited | Health & social care | 2021 |
| Fieldchester LPG Ltd | Wholesale & retail | 2014 |
| Fieldgreen Limited | Information & communication | 2010 |
| Fields Fruit & VEG Limited | Wholesale & retail | 2014 |
| Fifty Shades of Beauty Ltd | Other services | 2017 |
| Fight Life Boxing Limited | Arts & recreation | 2022 |
| Filter Free Limited | Professional & scientific | 2024 |
| Filter Services (UK) Limited | Wholesale & retail | 2007 |