| Distinctive Medical Products Ltd | Wholesale & retail | 2001 |
| Diversity Matters North West Ltd | Health & social care | 2007 |
| Dixon Rigby Keogh Limited | Professional & scientific | 2005 |
| Diya Marketing Ltd | Professional & scientific | 2024 |
| DJG Engineering Consultancy Limited | Administrative & support | 2007 |
| Djossou Limited | Information & communication | 1996 |
| DKL Kreative Options Limited | Administrative & support | 2008 |
| DKS Consultant Services Limited | Administrative & support | 2006 |
| DL Designs Limited | Professional & scientific | 1999 |
| DN Hearing Care Limited | Health & social care | 2026 |
| DO RE MI Day Nursery Limited | Education | 2013 |
| Doddington Park Farm (Nantwich) Limited | Extraterritorial | 2000 |
| Dollwell Limited | | 1981 |
| Dorset Head and Neck Surgery Limited | Health & social care | 2010 |
| DOS Services Limited | Wholesale & retail | 2003 |
| Dot.Connect Consulting Ltd | Professional & scientific | 2021 |
| Double Espresso & Friends Limited | Professional & scientific | 2006 |
| Double-Jay Consulting Ltd | Information & communication | 1998 |
| DOW Schofield Watts Business Recovery LLP | | 2014 |
| Downham Mayer Clarke Limited | Professional & scientific | 2003 |
| DR Doolittle Ltd | Administrative & support | 2006 |
| Dragon Petroleum Limited | Professional & scientific | 1956 |
| Drawbadge Drylining Limited | | 1988 |
| Drezlin Limited | Administrative & support | 2006 |
| Driven Network Services Ltd | Wholesale & retail | 2021 |
| Drummond CAD Services Ltd | Professional & scientific | 2015 |
| Dunham Forest Golf and Country Club Limited | Arts & recreation | 1958 |
| Dunkirk Landscape Services Limited | Administrative & support | 2004 |
| Durlston Services (UK) Ltd | Real estate | 2002 |
| Duscovent Engineering Limited | Construction | 1985 |
| Dusters Services Limited | Health & social care | 2002 |
| Dutta International Trading Limited | Professional & scientific | 1990 |
| Dutton Cooling Limited | Construction | 2017 |
| Dymofile Limited | | 1990 |
| E Delaney Demolition Ltd | Construction | 2006 |
| E J Hamilton Civil Engineering Services Limited | Construction | 2003 |
| E O G Building Services Ltd | Professional & scientific | 2026 |
| E-Flowers U.K. Limited | Wholesale & retail | 2002 |
| E-L Strategies Limited | Administrative & support | 2015 |
| E. G. Installations Limited | Construction | 2004 |
| E. Petch Printers Limited | Manufacturing | 1997 |
| E. Wilman & Sons Limited | | 1924 |
| E.Farrington & Co.Limited | Manufacturing | 1963 |
| E.G. for Business Ltd | Professional & scientific | 2007 |
| Eagle Control Engineering Limited | Manufacturing | 1978 |
| Eandr Asset Holdings Ltd | Financial services | 2025 |
| Eandr Group Holdings Ltd | Financial services | 2025 |
| Eandr Solutions NW Ltd | Financial services | 2025 |
| EAR Nourishment Limited | Information & communication | 2018 |
| Eastbeech Limited | Real estate | 1993 |
| Eastern Green Limited | Real estate | 1995 |
| Eastlake Builders Limited | Construction | 2018 |
| Eastway Salon (1959) Limited | | 1959 |
| Eaton Finance Limited | Financial services | 2003 |
| Eaton Masonic Hall (Congleton) Limited | Real estate | 1979 |
| Eaton Place (Hartford) Management Company Limited | Households | 2002 |
| EB Studio Ltd | Other services | 2026 |
| EB7 Limited | Professional & scientific | 2007 |
| EB7 Party Wall Limited | Professional & scientific | 2015 |
| Eccleston Green Residents Management Company Limited | Households | 2024 |
| ECD Architects Ltd | Professional & scientific | 1995 |
| Eclipse Health & Fitness Limited | Other services | 2003 |
| Eclipse Solar Control Limited | Construction | 2002 |
| Ecokem Limited | Manufacturing | 2003 |
| Ecolab (U.K.) Holdings Limited | Professional & scientific | 2002 |
| Econic Technologies Ltd | Professional & scientific | 2011 |
| Economic Research Services Limited | Professional & scientific | 1994 |
| Eddie Lighting Ltd | Information & communication | 2010 |
| Edge Squared Properties Limited | Real estate | 2022 |
| Edgeley Dental Practice Limited | Health & social care | 2005 |
| Educe Designs Limited | Professional & scientific | 1997 |
| Edwards Grounds Lettings Limited | Real estate | 1997 |
| EDY Promo Ltd | Transport & logistics | 2020 |
| Egertons Recovery Group Limited | Transport & logistics | 2010 |
| EGH Investments Limited | Real estate | 2010 |
| EGS Energy Services Limited | Wholesale & retail | 2003 |
| EIB Growth Services Limited | Extraterritorial | 2018 |
| EIB Professional Services Limited | Professional & scientific | 2012 |
| Eighty Eight Surgery Ltd | Health & social care | 2025 |
| EJ Projects Limited | Administrative & support | 2007 |
| Ejai Solutions Ltd | Information & communication | 2006 |
| EKT Consultancy Limited | Health & social care | 2024 |
| Elan Consulting Limited | Professional & scientific | 2012 |
| Electrical Combustion Engineering Consultancy Ltd | Professional & scientific | 1999 |
| Electrical Engineering & Technical Services Limited | Wholesale & retail | 1990 |
| Electrical Trading Company Limited | Construction | 2002 |
| Electrokinetic Limited | Construction | 2001 |
| Elevation Property Maintenance Limited | Construction | 2011 |
| Elias Enterprises Limited | Wholesale & retail | 1969 |
| Elior UK Holdings Limited | Professional & scientific | 1989 |
| Elior UK PLC | Hospitality | 1973 |
| Elior UK Services Limited | Administrative & support | 2004 |
| Elite Business and Finance Solutions Ltd | Professional & scientific | 2007 |
| Elite Health Limited | Arts & recreation | 2004 |
| Elite Rooms Limited | Real estate | 2010 |
| Elite Shower Services Limited | Administrative & support | 2005 |
| Elite Social Care Limited | Administrative & support | 2009 |
| Elkeeto Limited | Administrative & support | 2006 |
| Ellesmere Port Glass& Glazing Co. Limited | Construction | 1973 |
| Ellie and Andrew Bailey Limited | Professional & scientific | 2003 |