| Truesdale Engineering Limited | Construction | 2014 |
| Trufeeds Ltd | Administrative & support | 2026 |
| Truffle PIG Art Ltd | Arts & recreation | 2022 |
| Trulawn Gloucestershire Ltd | Construction | 2016 |
| Truly Tailored Recruitment Ltd | Administrative & support | 2016 |
| Trumans (Gotherington) Limited | Extraterritorial | 2020 |
| Trustedland Ltd | Information & communication | 2018 |
| Trusty Social Ltd | Administrative & support | 2022 |
| TRUTH001 Limited | Information & communication | 2019 |
| TS Phillips Limited | Agriculture & fishing | 2012 |
| Tshaw C S Ltd | Construction | 2019 |
| Tsjr Holdings Limited | Financial services | 2025 |
| TSM Wellness Limited | Professional & scientific | 2024 |
| Tspark Property Development Limited | Construction | 2013 |
| TSS Berrington Limited | Other services | 2022 |
| Ttka Property Investments Limited | Real estate | 2025 |
| Ttpeas Ltd | Professional & scientific | 2019 |
| Tube Science Ltd | Manufacturing | 2010 |
| Tudor Cookware Limited | Extraterritorial | 2021 |
| Tudor Engineering Consultancy Limited | Professional & scientific | 2024 |
| Tudor House Trustee Limited | Hospitality | 2021 |
| Tudoralbert Limited | Transport & logistics | 2019 |
| Tuffery Computing Limited | Information & communication | 2010 |
| Tugboat Advisory Ltd | Professional & scientific | 2016 |
| Tuiliere Limited | Administrative & support | 2025 |
| TUK2GO Limited | Hospitality | 2025 |
| Tukosupreme Ltd | Hospitality | 2022 |
| Tulip Designs Limited | Financial services | 1998 |
| Tumble Tots Gloucester LLP | | 2025 |
| Tumbleland Limited | Extraterritorial | 2013 |
| Tuneriver Ltd | Information & communication | 2018 |
| Tuning Talent Limited | Administrative & support | 2012 |
| Tunsrec Ltd | Wholesale & retail | 2023 |
| Tunstall House Management Company Limited | Households | 1991 |
| Tunstall Lodge Ltd | Households | 2014 |
| Turco Investment Ltd | Real estate | 2024 |
| Turf Wars Racing Limited | Arts & recreation | 2024 |
| Turned UP to 11 Ltd | Administrative & support | 2018 |
| Turner-Yates Enterprises Limited | Professional & scientific | 2015 |
| Turnkey Design Partnership Limited | Professional & scientific | 1981 |
| Turnkey Inventory Services Limited | Administrative & support | 2015 |
| Turnround Limited | Information & communication | 1990 |
| Turret Enterprises Limited | Extraterritorial | 2020 |
| Turret Property Ltd | Hospitality | 2024 |
| Turtle Tax Limited | Professional & scientific | 2026 |
| TVD UK Limited | Administrative & support | 2017 |
| TVM Cheltenham Ltd | Administrative & support | 2004 |
| TW Secure Data Ltd | Information & communication | 2025 |
| TWB Finishing Limited | Manufacturing | 1947 |
| Twcc Projects Ltd | Health & social care | 2024 |
| Twelve and Counting Limited | Professional & scientific | 2026 |
| Twelve Suffolk Square Limited | Households | 1988 |
| Twenty Lansdown Terrace RTM Company Limited | Administrative & support | 2004 |
| Twilight Enterprises Limited | Professional & scientific | 2025 |
| Twilight Fireworks Ltd | Arts & recreation | 2025 |
| Twins Hair Studio Limited | Real estate | 1979 |
| Twist of Silver Plumbing Ltd | Construction | 2016 |
| Twiston-Davies Bloodstock Limited | Agriculture & fishing | 2016 |
| TWM Creative Ltd | Professional & scientific | 2022 |
| Twnsnd-Testing Ltd | Education | 2025 |
| Two Alta Vista Limited | Real estate | 2014 |
| Two City Vegans Ltd | Information & communication | 2024 |
| Two Eighty Three Ltd | Information & communication | 2011 |
| Two Front Teeth Photography Limited | Professional & scientific | 2013 |
| Two ST Stephens Road Management Company Limited | Households | 2019 |
| Two Tivoli Road Management Limited | Households | 1997 |
| Twogate (Cheltenham) Limited | Real estate | 2017 |
| Twyning Thomases Limited | Arts & recreation | 2024 |
| Twyver Property Limited | Financial services | 2017 |
| Tycho Limited | Information & communication | 2023 |
| Tychonics Limited | Information & communication | 2023 |
| Tyga Food Company Limited | Hospitality | 2014 |
| Tyge Technologies Limited | Information & communication | 2024 |
| Tyler & Co. Salon Ltd | Other services | 2022 |
| Tylers Events Limited | Administrative & support | 2022 |
| Type UK Limited | Wholesale & retail | 1995 |
| Typecraft (Cheltenham) Limited | Manufacturing | 1997 |
| Tysr Leisure Ltd | Hospitality | 2022 |
| U&I Planning Ltd | Professional & scientific | 2022 |
| Uavtek Defence Ltd | Public administration | 2018 |
| Uavtek Limited | Professional & scientific | 2016 |
| Uavtek Production Limited | Manufacturing | 2021 |
| Uber Group Limited | Administrative & support | 2015 |
| Ubico Limited | Water & waste | 2011 |
| Ubique Heating & Renewables Ltd | Construction | 2025 |
| Ucal Exports Private Limited | Wholesale & retail | 2013 |
| Ucas Media Limited | Education | 1992 |
| Udemy UK Limited | Administrative & support | 2022 |
| Ugbug Limited | Real estate | 2023 |
| UJ Medical Limited | Health & social care | 2018 |
| UJ Property Holdings Limited | Real estate | 2023 |
| Ujio Consulting Limited | Administrative & support | 2013 |
| UK Adventure Rally Events Ltd | Arts & recreation | 2023 |
| UK and Irish Roots Limited | Other services | 2015 |
| UK Breakfast Limited | Hospitality | 2024 |
| UK Design Creative Limited | Professional & scientific | 2009 |
| UK Firearms Training Ltd | Education | 2021 |
| UK Gateway Property Ltd | Real estate | 2025 |
| UK Green Parks Development Limited | Hospitality | 2024 |
| UK Green Parks Ltd | Hospitality | 2022 |