| Townsend Builders Ltd | Construction | 2023 |
| Townsend Group Limited | Information & communication | 1995 |
| Toycentre Limited | Administrative & support | 1999 |
| TP Holdings Cheltenham Limited | Households | 2022 |
| TP Martech Limited | Information & communication | 2025 |
| TPO3 Ltd | Information & communication | 2022 |
| TQR Recruitment Co Ltd | Administrative & support | 2001 |
| TR7 Ltd | Wholesale & retail | 2023 |
| Track Access Productions Limited | Information & communication | 2001 |
| Traction Accounting Limited | Professional & scientific | 2024 |
| Trade OFF Ltd | Information & communication | 2026 |
| Tradebyte Software Limited | Information & communication | 2013 |
| Tradecraft Construction Limited | Construction | 2017 |
| Tradecraft Properties Limited | Real estate | 2021 |
| TRADES4U Maintenance Ltd | Construction | 2026 |
| Tradex Windows Limited | | 1984 |
| Tradexpert Ltd | Wholesale & retail | 2025 |
| Tradey Limited | Construction | 2019 |
| Traeth Bychan Caravan Co.Limited | Hospitality | 1964 |
| Traffic Group Signals Limited | Manufacturing | 2002 |
| Trail & Bloom Limited | Arts & recreation | 2026 |
| Training SAP Ltd | Information & communication | 2007 |
| TRAINING4FUSION Limited | Professional & scientific | 2008 |
| Tramm Property Development Ltd | Construction | 2025 |
| Transdermal Limited | Manufacturing | 2025 |
| Transfer 2 Limited | Information & communication | 2010 |
| Transformix Digital Ltd | Information & communication | 2024 |
| Transformr Operations Limited | Professional & scientific | 2019 |
| Transient Designs Ltd | Professional & scientific | 2005 |
| Transimeksa Limited | Transport & logistics | 2020 |
| Transport Design Ltd | Professional & scientific | 2019 |
| Transport Direct Europe Limited | Professional & scientific | 2001 |
| Tranter Transport Ltd | Transport & logistics | 2022 |
| Trapdoor Labs Limited | Information & communication | 2019 |
| Trapdoor Property Limited | Real estate | 2024 |
| Traqplan Limited | Information & communication | 2018 |
| Trask Legal Limited | Professional & scientific | 2025 |
| Trastevere Investments Limited | Real estate | 2013 |
| Trauma Reset CIC | Education | 2024 |
| Traumamed Solutions Limited | Wholesale & retail | 2023 |
| Travbox Limited | Arts & recreation | 2025 |
| Trazion Limited | Hospitality | 2025 |
| Tread Zone Ltd | Wholesale & retail | 2024 |
| Treaford (Cheltenham) Residents' Association Limited | Real estate | 1986 |
| Treats 4 U Cheltenham Ltd | Hospitality | 2021 |
| Trebus Technologies Limited | Professional & scientific | 2004 |
| Treby Limited | Information & communication | 2022 |
| Tredington Estates Limited | Real estate | 2018 |
| Tregenna Farm Ltd | Wholesale & retail | 2023 |
| Trelowen Veterinary Practice Limited | Professional & scientific | 2025 |
| Trench London Limited | Wholesale & retail | 2019 |
| Trengayor Farm Ltd | Real estate | 2020 |
| Tresmere Limited | Households | 1994 |
| Trew Engineering Ltd | Information & communication | 2024 |
| Trewsbury Farm Stud Limited | Agriculture & fishing | 2023 |
| TRI Climate Limited | Wholesale & retail | 2026 |
| TRI Sports Management Limited | Arts & recreation | 2015 |
| Tri-Health Group Ltd | Professional & scientific | 2016 |
| Tri-Q Group Limited | Professional & scientific | 2004 |
| Tribal Breath Ltd | Other services | 2022 |
| Tribal Resilience CIC | Extraterritorial | 2018 |
| Tribal Tracks Ltd | Hospitality | 2017 |
| Tribe Vintage Limited | Wholesale & retail | 2023 |
| Tribute Funerals Limited | Other services | 2023 |
| Trickbyte Games Limited | Information & communication | 2024 |
| Trikos Medical Limited | Health & social care | 2010 |
| Trillium Care Limited | Real estate | 2016 |
| Trilogy Business Support Limited | Information & communication | 2001 |
| Trimnasium Limited | Arts & recreation | 2011 |
| Trinity Cheltenham Trust Limited | Other services | 2008 |
| Trinsic Europe Ltd. | Administrative & support | 2021 |
| Trio Accountancy Services Ltd | Professional & scientific | 2022 |
| Trioak Capital Limited | Professional & scientific | 2025 |
| Triple 3 Group Limited | Information & communication | 2016 |
| Triple E Business Performance Coaching Limited | Professional & scientific | 2026 |
| Triple E Learning & Development Ltd | Information & communication | 2008 |
| Triple Five Invesments Ltd | Real estate | 2026 |
| Triskelion Advisory Ltd | Professional & scientific | 2024 |
| Triskelion Holdings & Investment Limited | Information & communication | 2023 |
| Tristone Consultants Ltd | Professional & scientific | 2019 |
| TRL Consultants Limited | Construction | 2013 |
| Trove Information Technologies UK Ltd | Information & communication | 2022 |
| Trower Davies Limited | Professional & scientific | 2007 |
| Troxler Labels Ltd | Information & communication | 2017 |
| Troy Steve Bloodstock Limited | Wholesale & retail | 1989 |
| TRS Cheltenham Ltd | Wholesale & retail | 2012 |
| TRS Healthcare International UK Limited | Other services | 2024 |
| TRU Fashion Ltd | Wholesale & retail | 2017 |
| TRU Recruitment Solutions Ltd | Administrative & support | 2018 |
| Trublue Technologies Ltd | Other services | 2022 |
| Truck Itco Ltd | Hospitality | 2026 |
| True Cotswold Ltd | Households | 2024 |
| True Cotswold Stays Ltd | Administrative & support | 2026 |
| True Fostering EOT Limited | Financial services | 2023 |
| True Fostering Limited | Health & social care | 2017 |
| True North Accountants Ltd | Professional & scientific | 2023 |
| True Talent Limited | Professional & scientific | 2023 |
| True Tone Music Ltd | Wholesale & retail | 2022 |
| Truebridge Care Ltd | Health & social care | 2025 |
| Trueline Builders Limited | Construction | 2016 |