| Tides Fish Bar Ltd | Hospitality | 2026 |
| Tiny Air Limited | Professional & scientific | 2018 |
| Torrs Hill Windfarm Limited | Professional & scientific | 1994 |
| Tourdethomo Ltd | Administrative & support | 2024 |
| Trackmanor Ltd | Information & communication | 2022 |
| True Circles Training Ltd | Education | 2021 |
| Turbine Repair Solutions Ltd | Manufacturing | 2012 |
| TW Qaqc Consultants Ltd | Professional & scientific | 2024 |
| United Bricks Hobby Shop Ltd | Wholesale & retail | 2024 |
| United Bricks Ltd | Manufacturing | 2022 |
| Vanguard Offshore Ltd | Transport & logistics | 2026 |
| Vaquita Management Services Limited | Administrative & support | 2017 |
| Vaughan Lawson Ltd | Professional & scientific | 2023 |
| Vaulkmill Ltd | Professional & scientific | 2022 |
| Velocis IT Ltd | Wholesale & retail | 2011 |
| Vogrie Pogrie C.I.C. | Arts & recreation | 2022 |
| W Bone Services Ltd | Manufacturing | 2019 |
| W R Wilson and Sons | Agriculture & fishing | 2014 |
| Wajs&Wajs Ltd | Construction | 2025 |
| Walk to the Edge Limited | Other services | 2007 |
| Wallace Road Tanks Ltd. | Transport & logistics | 2017 |
| Wallets' Marts Castle Douglas Limited | Other services | 1995 |
| Waukmill Farm Ltd | Agriculture & fishing | 2024 |
| WCT Services Ltd | Construction | 2024 |
| Webb Access Limited | Construction | 2023 |
| Wellie Chellie Limited | Agriculture & fishing | 2020 |
| Wellpark Scotland Limited | Information & communication | 2009 |
| Westpoint Design Consultants Limited | Wholesale & retail | 1981 |
| Westwind Drone Services Ltd | Professional & scientific | 2025 |
| Wheeler Groves Limited | Professional & scientific | 2015 |
| Where IS the Data? Ltd | Information & communication | 2019 |
| Windborne Limited | Professional & scientific | 2005 |
| Witchfest Limited | Other services | 2019 |
| Wjcr Ltd | Other services | 2014 |
| Wurst Christmas Limited | Wholesale & retail | 2024 |
| Zephir Limited | Manufacturing | 2007 |
| ZX Lidars Limited | Professional & scientific | 2008 |
| ZX Measurement Services Limited | Administrative & support | 2005 |
| Zydeco Limited | Information & communication | 1999 |
| Zynk Audio Ltd | Manufacturing | 2024 |