| RAA Style Barber Ltd | Other services | 2024 |
| Rarl Services Limited | Professional & scientific | 2022 |
| Riddick & SON (Builders) Limited | Construction | 1983 |
| Ringour Farmhouse | | 2003 |
| Riverside Trees | | 2000 |
| Riverview Park Limited | Construction | 1989 |
| Roan Properties Ltd | Real estate | 2026 |
| Robin Wallace & Partners | | 1994 |
| Ronnie Dodds Limited | Construction | 2014 |
| Root & Rise Wellbeing CIC | Arts & recreation | 2025 |
| Rothair Limited | Manufacturing | 2017 |
| Rubearb Ltd | Agriculture & fishing | 2025 |
| RW & CM Caravan Storage Limited | Arts & recreation | 2012 |
| Ryan's Rigging Ltd | Construction | 2026 |
| S C Geological Services Limited | Professional & scientific | 2017 |
| S J Solutions (Scotland) Ltd | Administrative & support | 2023 |
| Saint Property Limited | Real estate | 2001 |
| Salt & Pepe Limited | Hospitality | 2023 |
| SAM Goldie (Joinery) Ltd | Construction | 2022 |
| Sambradax Ltd | Wholesale & retail | 2019 |
| Sambuca Wine and Tapas Bar Ltd | Hospitality | 2025 |
| Sauna on the Hill Ltd | Other services | 2025 |
| SC Aviation Services Ltd | Manufacturing | 2024 |
| Scoffers Colvend Ltd | Hospitality | 2026 |
| Scott Country International Limited | Wholesale & retail | 2012 |
| Scott LEE Plant Hire Limited | Construction | 2017 |
| Scran-Tastic Ltd | Hospitality | 2017 |
| Scruffs Childcare Limited | Other services | 2010 |
| Scruffs Community Nursery Castle Douglas Community Interest Company | Other services | 2022 |
| Sea Gypsy Solutions Limited | Information & communication | 2025 |
| Sendout Distribution Ltd | Wholesale & retail | 2025 |
| Senwick House Limited | Health & social care | 2013 |
| Shade Salons Ltd | Other services | 2021 |
| Signdance Collective Limited | Arts & recreation | 2007 |
| Simon Harlow Design & Build Ltd. | Other services | 2016 |
| Simon Winstanley Associates Limited | Administrative & support | 2009 |
| SJH Inns Limited | Hospitality | 2015 |
| Skeabob Limited | Professional & scientific | 2026 |
| Skydrone Services Ltd | Agriculture & fishing | 2025 |
| SL000745 LP | | 1985 |
| SL004774 LP | | 2002 |
| Slagnaw Farming Limited | Agriculture & fishing | 2014 |
| Smiths & Scott Ltd | Manufacturing | 2024 |
| Solway Car Club Limited | Arts & recreation | 1986 |
| Solway Searchers Property Services Ltd | Real estate | 2020 |
| South West Engineering & Fabrications Limited | Manufacturing | 2018 |
| South West Storage Limited | Transport & logistics | 2016 |
| Southwest Conservatories Limited | Wholesale & retail | 2000 |
| Spring and Atlas Limited | Professional & scientific | 2020 |
| SQL Consulting UK Limited | Information & communication | 2024 |
| Standingstone Farm Limited | Agriculture & fishing | 2011 |
| Standingstone Farming Company Limited | Extraterritorial | 2012 |
| Star Enterprise & Co (Stranraer) Limited | Hospitality | 2016 |
| Starhemberg Ltd | Financial services | 2024 |
| Station House Cookery School Limited | Education | 2019 |
| Step by Step Hoof Trimming Ltd | Agriculture & fishing | 2025 |
| Stephen Gayford Services Ltd | Construction | 2021 |
| Stewartry Agricultural Society | Other services | 2008 |
| Stewartry Community Sports Club | Arts & recreation | 2012 |
| Stewartry Properties Ltd | Real estate | 2025 |
| Stewartry Rollershutters & Garage Doors Limited | Manufacturing | 2023 |
| Stewartry Veterinary Centre Limited | Professional & scientific | 2012 |
| Stocktex Limited | Wholesale & retail | 1977 |
| Strathken Services Ltd | Hospitality | 2021 |
| Strongwood Ltd | Construction | 2025 |
| Stryde Compliance Ltd | Professional & scientific | 2025 |
| Stryde Physiotherapy & Acupuncture Ltd | Health & social care | 2025 |
| Styles Ahead (CD) Limited | Other services | 2020 |
| Sulwath Brewers Limited | Manufacturing | 1996 |
| Summit to Sea Property Ltd | Real estate | 2023 |
| Supercontrol Limited | Information & communication | 2003 |
| Swift Solutions Scotland Ltd | Manufacturing | 2022 |
| Sypland Farms Limited | Agriculture & fishing | 2007 |
| T P Niven (Holdings) Limited | Other services | 2016 |
| T P Niven Limited | Transport & logistics | 2010 |
| T.B Turbine Solutions Ltd | Manufacturing | 2018 |
| Tapassdesignandprint Ltd | Manufacturing | 2024 |
| Taranaki Gate Limited | Information & communication | 2021 |
| THE Achie Farming Company | | 1997 |
| THE ARK Kippford Ltd | Hospitality | 2026 |
| THE Forestry Workers Co-Op Ltd | Agriculture & fishing | 2014 |
| THE FOX and Badger Emporium Ltd | Wholesale & retail | 2025 |
| THE Fullarton (Castle Douglas) Limited | Arts & recreation | 1993 |
| THE Galloway Wild Project Limited | Hospitality | 2025 |
| THE Glenisle Inn Palnackie Ltd | Hospitality | 2025 |
| THE Glenkens Community and Arts Trust Limited | Arts & recreation | 2001 |
| THE Granite Thistle Ltd | Hospitality | 2026 |
| THE Graphics Boat Ltd | Wholesale & retail | 2016 |
| THE Imperial Hotel (Castle Douglas) Ltd | Extraterritorial | 2010 |
| THE Little Optical Shop Ltd | Professional & scientific | 2020 |
| THE Livestock Consultants Ltd | Professional & scientific | 2025 |
| THE LOG House People Ltd. | Construction | 2008 |
| THE Natural Power Consultants Limited | Professional & scientific | 1997 |
| THE Olive Retreat Ltd | Hospitality | 2023 |
| THE Social Sport Network Ltd | Arts & recreation | 2024 |
| THE Tanning Boutique Enterprise Ltd | Other services | 2021 |
| Thorniehill Holding Company Limited | Other services | 2024 |
| Thorniehill Limited | Real estate | 2008 |
| Threave Rural Limited | Professional & scientific | 2015 |
| Threemerkland Farm | | 2000 |