| SR Property Finance Ltd | Financial services | 2022 |
| Stash Recovery Ltd | Transport & logistics | 2024 |
| Stay and Play Boarding at Thirdpart Kennels Ltd | Agriculture & fishing | 2021 |
| Steedman & Mulvey Limited | Construction | 2014 |
| Stevie Mills Training Ltd | Administrative & support | 2025 |
| Stewart Mortgage & Protection Solutions Ltd | Financial services | 2023 |
| Steyr Puch UK Limited | Extraterritorial | 2011 |
| Strathyre Estates Ltd | Real estate | 2026 |
| Street Level | Administrative & support | 1997 |
| Studio Kitchens & Bathrooms Ltd | Administrative & support | 2004 |
| Summerlea Ltd | Wholesale & retail | 2017 |
| Sunlions Enterprises Ltd | Agriculture & fishing | 2021 |
| Supreme Hospitality Ltd | Hospitality | 2025 |
| Susanne Mclaughlan Mortgage & Protection Solutions Ltd | Financial services | 2023 |
| SWB Innovations Ltd | Other services | 2018 |
| SWEETS4U Ltd | Wholesale & retail | 2020 |
| Systems Work Ltd | Information & communication | 2022 |
| T & H Transport Limited | Transport & logistics | 2023 |
| T Melrose Plumbing and Heating Limited | Construction | 2014 |
| T&Q Ltd | Real estate | 2024 |
| TEC GBR Ltd | Agriculture & fishing | 2020 |
| Teeuk Ltd | Wholesale & retail | 2023 |
| THE Attachment Company (Scotland) Ltd | Wholesale & retail | 2014 |
| THE Auld Store Bar Ltd | Hospitality | 2019 |
| THE Bubbles Factory Limited | Administrative & support | 2006 |
| THE Handy Garden MAN Limited | Administrative & support | 2026 |
| THE Happy Coach Company Ltd | Arts & recreation | 2020 |
| THE Health Hub App Ltd | Arts & recreation | 2026 |
| THE Lanark Stables Bistro Limited | Hospitality | 2015 |
| THE Long Run Club Ltd | Wholesale & retail | 2023 |
| THE Old Anvil Hair Room Ltd | Other services | 2019 |
| THE Oven @ Overton Farm Limited | Hospitality | 2014 |
| THE Quality Resolution Co. Ltd | Professional & scientific | 2025 |
| THE Shed Squad Ltd | Wholesale & retail | 2020 |
| THE Warren Eatery Ltd | Hospitality | 2024 |
| THE WEE Thackit (Carluke) Ltd | Hospitality | 2025 |
| Thomson Pettie Limited | Manufacturing | 2013 |
| Thomson Renewable Consultants Ltd | Manufacturing | 2020 |
| Tilliecat Ltd | Wholesale & retail | 2021 |
| Tindra Properties Ltd | Real estate | 2018 |
| Titanbears Limited | Agriculture & fishing | 2024 |
| TMD Logistics Ltd | Transport & logistics | 2014 |
| Todd Lifting Solutions Ltd | Construction | 2023 |
| TOM Young (Holdings) Limited | Professional & scientific | 1970 |
| TOM Young Limited | Manufacturing | 1968 |
| Tony Flynn Mortgages Limited | Financial services | 2020 |
| Trained for Life Limited | Education | 2019 |
| Travel With Joy Limited | Administrative & support | 2022 |
| Trostan Investments Limited | Real estate | 2019 |
| TSH (Financial Services) Ltd | Professional & scientific | 2013 |
| TSJ Data TEC Limited | Information & communication | 2024 |
| Tulogaz Ltd | Health & social care | 2025 |
| Unitech Electrical Limited | Construction | 1992 |
| Unitech Services Limited | Other services | 2005 |
| Unyco Ltd | Wholesale & retail | 2020 |
| Urban Boarding UK Ltd | Real estate | 2023 |
| Valhalla Property Rentals Limited | Real estate | 2023 |
| Van DE Vallei Stud Limited | Agriculture & fishing | 2018 |
| Velotek Ltd | Wholesale & retail | 2022 |
| VILLA247 Duquesa Limited | Households | 2014 |
| Vinetro Limited | Real estate | 2013 |
| VM Recovery Ltd | Wholesale & retail | 2022 |
| W Rentals Ltd | Real estate | 2021 |
| W&L Scotland Ltd | Real estate | 2022 |
| W. Young Appliance Repairs Ltd | Other services | 2026 |
| Walat Barber Ltd | Other services | 2024 |
| Waldencroft Ltd | Professional & scientific | 2015 |
| Wellriggs Ecology & Outdoor Learning Ltd | Professional & scientific | 2018 |
| Westcross Property Holdings Limited | Real estate | 2019 |
| WGA Rail Services Limited | Construction | 2018 |
| Which Mortgage Limited | Financial services | 1995 |
| Which Mortgage Properties Limited | Real estate | 2022 |
| William Davidson(Plumbers) Limited | Construction | 1971 |
| William Mcdonald Perry & Sons Limited | Real estate | 1989 |
| William Mcgregor & SON Limited | Agriculture & fishing | 2013 |
| Wilson Lifting Limited | Construction | 2017 |
| WJT Trades Limited | Construction | 2018 |
| WM Pringle & SON Ltd. | Transport & logistics | 2001 |
| Wood for Keeps Ltd | Manufacturing | 2026 |
| WP Fabrications Ltd | Manufacturing | 2026 |
| Writing Services Limited | Other services | 2016 |
| Xpedite Ltd | Professional & scientific | 2013 |
| Y2 Architecture Ltd | Professional & scientific | 2023 |
| Yieldshields Tree Surgeons Ltd | Agriculture & fishing | 2011 |
| ZERO4FORTY Ltd | Information & communication | 2024 |
| ZK Roofing Services Ltd | Construction | 2025 |