| 3GL Transport (Scotland) Limited | Transport & logistics | 2011 |
| A & C Adams Ltd | Transport & logistics | 2018 |
| A & J Ralston Limited | Construction | 2003 |
| A Dog's Best Friend Ltd | Other services | 2021 |
| A. Adams (Haulage) Ltd | Transport & logistics | 2015 |
| A.A. Scott Holdings Ltd | Financial services | 2019 |
| A.D.R Scotland Ltd | Construction | 2021 |
| A.G.M. Properties (Borders) Ltd | Real estate | 2024 |
| AAA Trades Limited | Construction | 2012 |
| Aaronswood Properties Limited | Real estate | 1999 |
| Abode Hamilton Ltd | Wholesale & retail | 2025 |
| Acform Investments Limited | Real estate | 2008 |
| Adept Homes Ltd | Construction | 2019 |
| Advance ESS Limited | Professional & scientific | 2025 |
| Adxi Solutions Ltd | Wholesale & retail | 2025 |
| AER Global Limited | Wholesale & retail | 2025 |
| AFQ Foods Ltd | Other services | 2025 |
| AG Builder (Scotland) Limited | Construction | 2024 |
| AG Electrical & Security Ltd | Construction | 2008 |
| AG MOT Ltd | Wholesale & retail | 2025 |
| AIG (Joiners & Builders) Limited | Construction | 2025 |
| Aigmuir Ltd | Health & social care | 2026 |
| AIM High 10 Limited | Extraterritorial | 2026 |
| Airdrie Fades Ltd | Other services | 2026 |
| Aire Plumbing & Heating Ltd | Construction | 2026 |
| Aitken Autotec Ltd | Wholesale & retail | 2025 |
| AJ Design Group Ltd | Professional & scientific | 2021 |
| Akura Group Limited | Wholesale & retail | 2020 |
| Alan White Ltd | Wholesale & retail | 2023 |
| ALT Construction (Carluke) Ltd | Construction | 2025 |
| AMH Enterprises Ltd | Wholesale & retail | 2025 |
| AMR Training Associates Ltd | Other services | 2024 |
| Andrew Chapman Plumbing & Heating Engineer Ltd | Construction | 2022 |
| Antho Hair and Beauty Ltd | Other services | 2012 |
| Anubis Crowd Management & Events Ltd | Administrative & support | 2024 |
| Anubis Group (FM) Ltd | Administrative & support | 2022 |
| Anubis Group Training Academy Ltd | Education | 2022 |
| Anubis Security Ltd | Administrative & support | 2014 |
| APG Facilities Ltd | Construction | 2021 |
| Aras Barbers Carluke Ltd | Other services | 2025 |
| Ardmore Trustee Services Ltd | Extraterritorial | 2010 |
| ARK Bridal Limited | Other services | 2025 |
| AS Street Food Limited | Hospitality | 2024 |
| Asiatandooricarluke Ltd | Hospitality | 2026 |
| Athol Industrial Estate Ltd | Real estate | 2025 |
| Atholl Houses Limited | | 1944 |
| Automated Access Control Ltd | Manufacturing | 2013 |
| AVC Construction Ltd | Construction | 2014 |
| Aviemore Independent Ltd | Hospitality | 2018 |
| AWH Joinery & Building Services Ltd | Construction | 2019 |
| B of D Ltd | Administrative & support | 2020 |
| Barn Buddies Community Interest Company | Health & social care | 2020 |
| Bedford Falls Ltd. | Information & communication | 2003 |
| BEEBEE2 Ltd | Hospitality | 2019 |
| Belhaven Autos Ltd | Wholesale & retail | 2026 |
| Belhaven Cars Sales Ltd | Wholesale & retail | 2022 |
| Benz Manufacturing Consultancy Limited | Professional & scientific | 2013 |
| Besp-Oak Interiors (Scotland) Ltd | Wholesale & retail | 2018 |
| Bethune Property Holdings Ltd | Real estate | 2018 |
| BGD Contractors Ltd | Professional & scientific | 2025 |
| Bidmac Consultancy Ltd | Professional & scientific | 2026 |
| Big Trudy Limited | Real estate | 2025 |
| Bill Kelly Developments Limited | Construction | 2024 |
| Bill Property Investments Limited | Real estate | 2024 |
| Blair Field Services Limited | Agriculture & fishing | 2016 |
| Blair Hallage Ltd | Transport & logistics | 2020 |
| Blanestaxi Service | | 2002 |
| Blind Logic Limited | Wholesale & retail | 2017 |
| Blind Logic Online Ltd | Wholesale & retail | 2020 |
| Bloom Evolve Ltd | Information & communication | 2026 |
| Bluberry Marketing Ltd | Other services | 2018 |
| Bonito Baby Ltd | Wholesale & retail | 2020 |
| Bowhouse Consultancy Services Limited | Professional & scientific | 2019 |
| Brennan Offsite Service Solutions Ltd | Professional & scientific | 2018 |
| Briangela Ltd | Professional & scientific | 2018 |
| BRJ Architecture Ltd | Professional & scientific | 2024 |
| Brooks House Studios Ltd | Arts & recreation | 2026 |
| Brownlee House Ltd | Other services | 2024 |
| Bryce Electrical Services Ltd | Construction | 2014 |
| Budget Vehicle Leasing Ltd | Transport & logistics | 2026 |
| Bugatti Ltd | Hospitality | 2021 |
| Burn Road Garage Limited | Wholesale & retail | 2010 |
| Burnhouse 2022 Limited | Wholesale & retail | 2022 |
| Burnley Growth Partners Limited | Professional & scientific | 2026 |
| Burns Electrical Services Ltd | Manufacturing | 2017 |
| Bushelhead Farms Limited | Agriculture & fishing | 2026 |
| C J Electrical Contractors Ltd | Construction | 2012 |
| C Scott & Sons Limited | Transport & logistics | 2017 |
| C Z Joinery Ltd | Construction | 2025 |
| C.E.H Civils Ltd | Construction | 2025 |
| Cabbrecoveryandrepairs Ltd | Wholesale & retail | 2025 |
| Cafe ST. Giles Ltd. | Hospitality | 2011 |
| Caledonia Biotech Solutions Limited | Professional & scientific | 2019 |
| Caley Bar Limited | Hospitality | 2017 |
| Call Point Limited | Administrative & support | 2022 |
| CAM Flooring and Building Limited | Construction | 2024 |
| Canonach Project Management Limited | Administrative & support | 2003 |
| Cans BOT Limited | Wholesale & retail | 2019 |
| Carbon Coaching Ltd | Arts & recreation | 2022 |
| Care Matters Social Work Consultancy Ltd | Health & social care | 2026 |