| Spartan Transport & Logistics Ltd | Transport & logistics | 2020 |
| Spawty Paws Agility Ltd | Arts & recreation | 2026 |
| SPC Financial Management Limited | Professional & scientific | 2020 |
| Specialist Tradesmen Midlands Limited | Construction | 2017 |
| Spectrum Games Limited | Arts & recreation | 2004 |
| Speedies Ltd | Transport & logistics | 2022 |
| SPEEDY2GO Ltd | Transport & logistics | 2016 |
| Spell Book and Candle Ltd | Wholesale & retail | 2026 |
| Spencer G Wright Limited | Extraterritorial | 2013 |
| Spencer’S Electrical Contractors Ltd | Construction | 2017 |
| Sphereco Limited | Information & communication | 2022 |
| Sphumanelements Ltd | Administrative & support | 2021 |
| Spiceland Burton Ltd | Hospitality | 2024 |
| Spiceroot Traders Ltd | Wholesale & retail | 2025 |
| Spicy Bites (Burton) Ltd | Hospitality | 2026 |
| Spiritually Social Ltd | Information & communication | 2022 |
| Splice of Lime Ltd | Professional & scientific | 2023 |
| Sports Centred Investments UK Limited | Real estate | 2019 |
| Sports Loop Ltd | Arts & recreation | 2025 |
| Spotless Domestic Cleaners Limited | Administrative & support | 2025 |
| Spotlight Fraud & Cyber Risk Consulting Limited | Extraterritorial | 2021 |
| SPR Property Maintenance & Renovations Ltd | Administrative & support | 2022 |
| Spring Chop Suey Food Ltd | Hospitality | 2025 |
| Springfield of Burton Limited | Wholesale & retail | 1973 |
| Springs Commercial Cleaning Services Limited | Administrative & support | 2002 |
| Spud Heaven Midlands Ltd | Hospitality | 2025 |
| Square Technology Designs Limited | Administrative & support | 2025 |
| Squirrel Investment Services Limited | Real estate | 2020 |
| Squirrell Plant & Transport Limited | Transport & logistics | 2015 |
| SSS Midlands Group Ltd | Financial services | 2022 |
| ST Barbares Ltd | Other services | 2024 |
| ST Chads 1951 Limited | Extraterritorial | 2013 |
| ST John’S Road Smalley Management Company Limited | Households | 2025 |
| ST Modwen Medical Consultancy Limited | Health & social care | 2026 |
| ST Peters Barbers Ltd | Other services | 2023 |
| ST Swithins Apartments Management Ltd | Real estate | 2017 |
| ST West Properties Ltd | Real estate | 2021 |
| ST. Mary's Investments Limited | Real estate | 1956 |
| ST. Vincent Healthcare, Cleaning & Logistics Ltd | Transport & logistics | 2025 |
| ST14 Properties Ltd | Real estate | 2024 |
| Staff Connect Solutions Ltd | Administrative & support | 2024 |
| Staff Mate Limited | Administrative & support | 2023 |
| Staffing Specialists Ltd | Administrative & support | 2025 |
| Stafford Electrical Testing Limited | Professional & scientific | 2018 |
| Staffordshire Cost Consultants Ltd | Professional & scientific | 2023 |
| Staffordshire Kindling Supplies Ltd | Agriculture & fishing | 2013 |
| Staffs Sports Massage Ltd | Other services | 2024 |
| Stage Fever Productions Ltd | Arts & recreation | 2021 |
| Stanciumirela Ltd | Other services | 2022 |
| Stanhope Green Management Limited | Households | 2025 |
| Stapenhill Football Club Limited | Arts & recreation | 1996 |
| Stapenhill Fryer and Pizza Ltd | Hospitality | 2025 |
| Stapenhill PO Ltd | Wholesale & retail | 2025 |
| Star Building and Heating Solutions Ltd | Construction | 2023 |
| Star Fashion Ltd | Wholesale & retail | 2026 |
| Starling Endeavours Limited | Professional & scientific | 2025 |
| Starling Therapeutic Care Services (Burton) Ltd | Health & social care | 2017 |
| Stars Courier Ltd | Transport & logistics | 2025 |
| Startin Properties Limited | Real estate | 1965 |
| Station Studios Ltd | Other services | 2024 |
| Stay Just Tonight Ltd | Real estate | 2024 |
| Stay Smart Rentals Ltd | Real estate | 2025 |
| STB Contracting Limited | Professional & scientific | 2013 |
| Steel Buildings Design and Build Limited | Construction | 2015 |
| Steel Framed Services (Installations) Limited | Construction | 2016 |
| Steel Merchants Limited | Wholesale & retail | 2011 |
| Stelmar Consultancy Ltd | Financial services | 2018 |
| Stemik Gaming Limited | Wholesale & retail | 1998 |
| Step PI Associates Limited | Professional & scientific | 2012 |
| Sterling Beacon Financial Ltd | Administrative & support | 2025 |
| Steva Transport Ltd | Transport & logistics | 2019 |
| Steve Sandiford Consulting Limited | Professional & scientific | 2018 |
| Steven Hall Information Technology Services Limited | Information & communication | 2016 |
| Stevensons BUS Services Ltd | Transport & logistics | 2023 |
| Still Management Limited | Professional & scientific | 2023 |
| Stirclean Services Limited | Administrative & support | 2026 |
| Stone Lettings Ltd | Real estate | 2017 |
| STR Car Ltd | Wholesale & retail | 2025 |
| Strade Investments Ltd | Administrative & support | 2022 |
| Straightforward Haulage Limited | Transport & logistics | 2017 |
| Strategic Arrow Limited | Professional & scientific | 2018 |
| Strategic Financial Consultants Limited | | 1989 |
| Strategic Financial Planners Limited | Financial services | 2016 |
| Strategic Healthcare Associates Limited | Extraterritorial | 2014 |
| Stratos Supply Chain Consulting Ltd | Professional & scientific | 2025 |
| Stratus Education Limited | Education | 2016 |
| Stravoos Ltd. | Administrative & support | 2016 |
| Strawberry Fields Properties Limited | Real estate | 2025 |
| Strawberry Fields Qmover Ltd | Wholesale & retail | 2022 |
| Stretton Day Nursery Limited | Education | 2015 |
| Stretton Hair CUT Ltd | Other services | 2024 |
| Stride Construction Ltd | Construction | 2024 |
| Strong Asset Management Ltd | Wholesale & retail | 2024 |
| Structured Cabling Solutions Ltd | Information & communication | 2017 |
| Strutt Import Export Limited | Wholesale & retail | 2013 |
| Stuart Birt Consultancy Ltd | Professional & scientific | 2024 |
| Stuart James Systems Limited | Manufacturing | 1979 |
| Stuart Property Investments Ltd | Real estate | 2025 |
| Stuart Walton Limited | Extraterritorial | 2015 |
| Stuarts Flooring Limited | Extraterritorial | 2013 |