| Photographer Scotland Ltd | Information & communication | 2023 |
| Pm&S Property Limited | Real estate | 2025 |
| Poison Burntisland Ltd | Other services | 2022 |
| Possible Paths CIC | Information & communication | 2026 |
| Post and Pantry Limited | Wholesale & retail | 2016 |
| Prestige Comps Limited | Extraterritorial | 2023 |
| Property Gems Ltd | Real estate | 2012 |
| Property Rentalzzz Ltd | Real estate | 2023 |
| Protea Rentals Ltd | Real estate | 2021 |
| Purpledup Limited | Wholesale & retail | 2021 |
| Quality Prime Cuts Ltd | Wholesale & retail | 2020 |
| R Nails & Spa Ltd | Other services | 2026 |
| Rae's Anatomy Mind and Movement Limited | Health & social care | 2026 |
| Rankin Funeral Directors Burntisland Limited | Other services | 2022 |
| React & Maintain Limited | Administrative & support | 2008 |
| Red Carpet Jewellers Ltd | Wholesale & retail | 2014 |
| RL Medical Expert Ltd | Health & social care | 2023 |
| Romafi Systems Limited | Information & communication | 2015 |
| Roof Force Ltd | Construction | 2007 |
| Rosy Lane Ltd | Wholesale & retail | 2021 |
| Sane Taxis Limited | Professional & scientific | 2007 |
| Saow Properties Ltd | Real estate | 2023 |
| SCH Structures Limited | Professional & scientific | 2007 |
| Scotia GAS Care Limited | Construction | 2008 |
| Scotus Property Investments Limited | Real estate | 2020 |
| Seaside Place Management Ltd | Administrative & support | 2023 |
| Selkirk Hill Investments Limited | Real estate | 1994 |
| Sell to Retire Limited | Administrative & support | 2025 |
| Seren Digital Ltd | Information & communication | 2026 |
| Sheiling Hideaways Ltd | Real estate | 2025 |
| Shsl EOT Trustees Limited | Financial services | 2021 |
| Sinclair Inspections Ltd | Construction | 2025 |
| SKB Design Consultants Ltd | Professional & scientific | 2024 |
| SLC Medical Consulting Limited | Professional & scientific | 2023 |
| SM Landscaping Services Ltd | Administrative & support | 2023 |
| Small Wonders Yarn and Crafts Ltd | Wholesale & retail | 2025 |
| Smart Electrics Contractors Ltd | Manufacturing | 2010 |
| Smart Properties (Fife) Ltd | Real estate | 2023 |
| Smash Brow Ltd | Administrative & support | 2019 |
| SME Business Advisory Services Limited | Professional & scientific | 2018 |
| Smith & Horner, Limited | Wholesale & retail | 1960 |
| Solus Holistic Ltd | Health & social care | 2025 |
| SOW 'N Grow CIC | Education | 2023 |
| Sparklinx Ltd | Professional & scientific | 2025 |
| Spice Lounge Holdings Limited | Real estate | 2022 |
| Sscassessments Ltd | Education | 2023 |
| Starley Hall Holdings Ltd | Financial services | 2021 |
| Starley Hall School Limited | Health & social care | 1998 |
| Stencil Pro Limited | Manufacturing | 2025 |
| Stephen White Developments Ltd. | Construction | 2000 |
| Stirling Simulation Services Limited | Professional & scientific | 2017 |
| Sunrise Bakehouse Limited | Manufacturing | 2025 |
| Syntegra (Operational Marketing Consultancy) Limited | Other services | 2001 |
| TGC Capital Ltd. | Real estate | 2018 |
| THE Emerson Partnership Limited | Professional & scientific | 2021 |
| THE Essi & Isle Co Ltd | Wholesale & retail | 2021 |
| THE Evolve Group (Scotland) Limited | Manufacturing | 2006 |
| THE Happy Go Lucky Dog Company Limited | Wholesale & retail | 2016 |
| THE Kinghorn Ship Ltd | Hospitality | 2023 |
| THE Nail Potion Ltd | Other services | 2022 |
| TIM Wenman Consulting Ltd | Professional & scientific | 2025 |
| Time for YOU (Fife) Limited | Other services | 2008 |
| TOM Courts Quality Foods (Fife) Ltd | Wholesale & retail | 2020 |
| Tornado Bagel Limited | Hospitality | 2025 |
| Toynton Joinery Limited | Construction | 2004 |
| Travel Island Ltd | Administrative & support | 2023 |
| Tredly Ltd | Wholesale & retail | 2024 |
| TS Structural Ltd | Professional & scientific | 2022 |
| Two Skies Ltd | Manufacturing | 2014 |
| Uxtv Limited | Information & communication | 2023 |
| Value Base Limited | Professional & scientific | 2012 |
| Walters Family Helping Services Limited | Real estate | 2026 |
| Wardlaw Engineering Ltd | Construction | 2025 |
| Wats-In.Store Ltd | Wholesale & retail | 2017 |
| Wattersway Ltd | Hospitality | 2020 |
| What the Fork Ltd | Information & communication | 2018 |
| Where Holdings Ltd | Wholesale & retail | 2021 |
| Where Ltd | Wholesale & retail | 2022 |
| William Angus Limited | Arts & recreation | 2024 |
| Woodside Garage Ltd | Wholesale & retail | 2015 |
| Wrfc Wind Ltd | Construction | 2025 |
| Wylie Tech Ltd | Wholesale & retail | 2019 |
| Xtreme Powder Coating Ltd | Administrative & support | 2017 |
| Yggdrasil Entertainment International Ltd | Information & communication | 2024 |